癌的遗传易感性与辐射致癌

综述了家族性癌基因的种类及其作用机制，并对其与辐射致癌的关系进行了探讨，进而通过流行病学研究资料所获得的证据，认为在人群中存在对特定肿瘤易感的遗传学亚群，该亚群可能对辐射致癌（指上述特定肿瘤）高敏感。     

AN EPIDEMIOLOGY AND MOLECULAR GENETIC STUDY ON BREAST CANCER SUSCEPTIBILITY

ＩＮＴＲＯＤＵＣＴＩＯＮ　　Ｂｒｅａｓｔｃａｎｃｅｒｉｓｏｎｅｏｆｔｈｅｍｏｓｔｃｏｍｍｏｎｃａｎｃｅｒａｎｄｃａｕｓｅｏｆｄｅａｔｈｉｎｗｏｍｅｎａｒｏｕｎｄｔｈｅｗｏｒｌｄ .Ｔｈｅｒｅｉｓａｆｏｕｒｔｏｆｉｖｅｆｏｌｄｖａｒｉａｔｉｏｎｉｎｔｈｅｉｎｃｉｄｅｎｃｅｒａｔｅｓａｃｒｏｓｓｄｉｆｆｅｒｅｎｔｃｏｕｎｔｒｉｅｓ.ＴｈｅｌｏｗｅｓｔｒａｔｅｓａｒｅｏｂｓｅｒｖｅｄｉｎＡｓｉａ ,ａｎｄｔｈｅｈｉｇｈｅｓｔｒａｔｅｓａｒｅｏｂｓｅｒｖｅｄｉｎｗｅｓｔｅｒｎＥｕｒｏｐｅａｎｄＮｏｒｔｈＡｍ…     

齐刺温针肩内俞治疗肩关节周围炎100例临床观察

目的　观察齐刺温针肩内俞治疗肩关节周围炎的临床疗效。方法　齐刺温针肩内俞治疗肩关节周围炎 1 0 0例 (治疗组 ) ,并与常规针刺治疗 1 0 0例 (对照组 )进行对照观察。结果　临床治愈率、总有效率治疗组分别为 78.0 0 %、99.0 0 % ,对照组分别为 61 .0 0 %、87.0 0 % ,2组比较有显著性差异 (P <0 .0 1 )。治疗组在缓解疼痛、改善肩关节活动、提高患者日常生活能力及缩短疗程方面均明显优于对照组 ( P <0 .0 1 )。结论　齐刺温针肩内俞治疗肩关节周围炎疗效确切 ,能较快缓解疼痛 ,改善肩关节功能。     

中西医结合治疗急性病毒性心肌炎116例疗效观察

目的观察中西医结合治疗急性病毒性心肌炎的疗效。方法对211例临床诊断为急性病毒性心肌炎的患者随机分为两组,治疗组116例,在常规西药治疗(极化液、抗心律失常药物、抗病毒等)的基础上,加用本院自己配方研制的归脾养心冲剂;对照组95例,采用常规西药治疗。结果治疗组在治愈率、总有效率方面均优于对照组(P<0.01、P<0.05)。结论中西医结合治疗急性病毒性心肌炎可显著提高临床疗效。     

先矫形后针刺法治疗周围性面神经麻痹100例临床观察

目的: 观察不同针刺方法对周围性面神经麻痹的疗效.方法: 将200例周围性面神经麻痹患者随机分为2组,分别采用先矫形后针刺法(治疗组)和常规针刺法(对照组)进行治疗.结果: 治疗组愈显率为97-0%,对照组愈显率为78-0%,治疗组疗效优于对照组(P＜0-01).结论: 先矫形后针刺法治疗周围性面神经麻痹优于常规针刺法.     

细胞色素P450 CYP1A1基因多态与鼻咽癌易感性的关联分析

Objective To investigate the association between CYP1A1 gene polymorphisms and susceptibility of nasopharyngeal carcinoma in Cantonese nuclear families through family-based association study. Methods A total of 457 Cantonese nuclear families, consisting of 2134 members, were recruited as subjects. Each family included two parents and at least one offspring with nasopharyngeal carcinoma. Two single nucleotide polymorphisms (SNP) in CYP1A1 named m1 (rs4646903) and m2 (rs1048943) , were genotyped by PCR-RFLP assay and verified by directly sequencing. The genotype data were analyzed with family-based association test (FBAT) software to check the linkage and association between the two genetic markers and susceptibility of nasopharyngeal carcinoma. Results FBAT analysis showed that the minor allele frequencies (MAF) of the two SN P were 0. 442 (C) and 0. 339 (G) respectively. For m1 polymorphism in CYP1A1 gene was not significantly associated with nasopharyngeal carcinoma in our study population whether stratified with VCA-IgA or not (without stratification : X2=2. 399, P=0. 301 ; with stratification : Iow-titer group (VCA-IgA<1 : 80), MAF=0. 457 (C), X2=1.221, P=0.543 ; high-titer group (VCA-IgA ≥1 : 80), MAF=0. 427 (C), X2=2. 832, P=0. 243). For m2 polymorphism, when VCA-IgA<1 : 80, the G allele showed decreased transmission under additive and dominant model (MAF=0. 347 (G) ; Zadditive=-2. 120,Padditive=0. 034;Zdominant=-2. 303,Pdominant=0.021)and a boundary P value was got with global statistic (X2=5. 394, P=0. 067). Haplotype TG (0. 057), constructed by ml and m2, might decrease nasophargneal carcinoma risk (Z=-2. 002,P=0. 045). A boundary P value was also got with global statistic (X2=7. 067 ,P=0. 070). Conclusion There was no statistical significance between ml polymorphism and susceptibility of nasopharyngeal carcinoma in Cantonese nuclear families. And this study showed that m2 polymorphism might associated with the decrease of nasopharyngeal carcinoma in Cantonese nuclear families.     

细胞色素P450 CYP1A1基因多态与鼻咽癌易感性的关联分析

目的 利用以核心家系为基础的关联研究探讨细胞色素P450酶系(cytochrome P450,CYP450)CYP1A1基因m1和m2多态性并分析其与鼻咽癌易感性的关联.方法 收集457个广东鼻咽癌核心家系(每个核心家系由患者和父母或同胞构成)共2134名成员作为研究对象,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对CYPlAl基因单核苷酸多态性(SNP)位点m1和m2(其参考编号分别为rs4646903和rs1048943)进行基因分型.挑选PCR产物测序验证.运用家系为基础的相关性检验(family-based association test,FBAT)软件分析这两个多态位点的基因型及其单体型与鼻咽癌易感性的关联.结果 FBAT软件分析结果 显示,位点m1和m2的微效等位基因频率(MAF)分别为0.442(C)和0.339(G).其中无论是否根据EB病毒壳抗原抗体(VCA-IgA)分层,m1位点与鼻咽癌的易感性之间关联均无统计学意义[未分层:X2=2.399,P=0.301;分层后:低滴度组(VCA-IgA<1:80),MAF=0.457(C),X2=1.221,P=0.543;高滴度组(VCA-IgA≥1:80),MAF=0.427(C),X2=2.832,P=0.243];对m2位点来说,末根据VCA-IgA分层时,该位点与鼻咽癌的易感性之间关联无统计学意义(X2=2.694,P=0.260).分层后,在低滴度组,累加和显性模式下,位点m2等位基因G显示了从亲代到子代传递减少[MAF=0.347(G);Z<,累加>=-2.120,P<,累加>=0.034;Z显性=-2.303,P显性=0.021],全局统计也提示了传递的改变(X2=5.394,P=0.067);由这两个位点构建的单体型TG(0.057)可能降低鼻咽癌的发病风险(Z=-2.002,P=0.045),全局统计也提示CYP1A1基因单体型可能与鼻咽癌易感性有关系(X2=7.067,P=0.070).结论 以家系为基础的相关性研究发现CYP1A1基因多态位点m1与鼻咽癌的易感性之间关联无统计学意义,位点m2基因多态性可能与鼻咽癌的发病风险降低有关系.     

乳腺癌家族聚集性的遗传流行病学研究——危险因素、分离比、遗传度分析

目的 研究遗传因素在乳腺癌发生中的作用。方法 采用１：１匹配的病例对照研究方法，。从我国四个城市的主要医院收集了１９９６年１０月￣１９９７年１月间入院的乳腺癌新发病例及其对照计３１３对，并对其进行家系调查。采用Ｌｏｇｉｓｔｉｃ回归分析方法进行乳腺癌危险因素分析。采用Ｌｉ－Ｍａｎｔｅｌ－Ｇａｒｔ法进行乳腺癌分离比的估算，采用Ｆａｌｃｏｎｅｒ回归法进行遗传度估算。结果 乳腺部家族史是一显著的危险因素，