金边瑞香浸膏对小鼠血清IFN-γ、IL-4、IL-18表达的影响

目的观察金边瑞香（daphne odora var.marginata,DOVM）浸膏对小鼠血清干扰素γ（IFN-γ）、白细胞介素4（IL-4）、白细胞介素18（IL-18）表达的影响。方法选用昆明系小鼠60只,随机分为生理盐水组（对照组）和1、2、4、8、16 g/（kg.d）DOVM浸膏组共6组,每组10只。DOVM浸膏不同浓度组分别灌胃给药0.02 mL/g,1次/d,连续14 d;生理盐水组给予生理盐水灌胃0.02 mL/g,1次/d,连续14 d。常规分离血清后,采用生物素双抗体ELISA夹心法（ABC-ELISA）测定各组小鼠血清IFN-γ、IL-4、IL-18的水平。结果1 g/（kg.d）DOVM浸膏组血清IL-4水平明显高于生理盐水组（P〈0.05）。生理盐水组与DOVM浸膏不同浓度组血清IFN-γ、IL-18水平比较差异均无统计学意义（P均〉0.05）,DOVM浸膏不同浓度组间血清IFN-γ、IL-18水平比较差异也均无统计学意义（P均〉0.05）。结论1 g/（kg.d）DOVM浸膏对小鼠IL-4的表达有一定促进作用。     

脊蛛网膜下腔置管引流治疗蛛网膜下腔出血30例分析

１９９７１０～１９９８３期间，我们采用脊蛛网膜下腔置管引流法治疗蛛网膜下腔出血（ＳＡＨ）患者３０例，获得较满意疗效，现报道于下：１临床资料与方法１１临床资料本组３０例患者，男１９例，女１１例；年龄：３０～６５岁，平均４７５岁；发病至接受治疗的...     

微创术结合持续脑室滴注灌洗清除脑室内血肿

2002年11月至2003年10月，作者采用YL-1型颅内血肿粉碎穿刺针(北京万特福公司生产)对脑室内血肿患者行脑室微创引流，并持续滴注等渗盐水加尿激酶灌洗脑室，以加速清除脑室内血肿，现报道如下。     

核苷(酸)类似物治疗慢性乙型肝炎患者HBV逆转录酶区变异的研究

目的分析核苷(酸)类似物治疗的慢性乙型肝炎患者HBV逆转录酶区耐药相关变异位点、变异位点组合模式、HBV DNA水平和HBeAg等特征,为临床应用核苷(酸)类似物抗乙肝病毒治疗提供参考。方法纳入研究的141例慢性乙型肝炎患者,使用NAs治疗的107例为治疗组,未使用药物抗病毒治疗的34例为对照组。采用线性探针反向杂交法(PCRRLP)检测HBV逆转录酶区耐药相关变异位点,实时荧光定量PCR法检测乙型肝炎病毒DNA水平,酶联免疫吸附试验检测乙肝免疫标记物,并对耐药相关变异的特点和研究病例的临床资料进行统计学分析。结果核苷(酸)类似物治疗效果不佳组耐药相关变异检出率(82.5%)明显高于未治疗的对照组(5.9%)和抗病毒治疗有效组(有效组10例未检出耐药相关变异)(P0.01)。97例核苷(酸)类似物抗病毒效果不佳的病例中80例检出140个耐药相关变异位点,包括rtM204I 45个、rtM204V 32个、rtA181V 28个、rtL180M 25个、rtN236T 10个;变异位点组合模式主要有:rtL180M+rtM204I、rtM204V、rtM204I+rtM204V、rtA181V+rtM204V、rtM204I、rtA181V+rtN236T等;51个对NAs应答不佳病例耐药变异检出率70.59%,46个病毒学突破病例耐药变异检出率95.65%,差异明显(P0.01)。NAs单药治疗病例早于多药治疗病例检出耐药相关位点(P0.01);NAs治疗时间越长,耐药变异检出率越高(P0.01);病例HBV-DNA水平等级越高,耐药变异检出率也越高(P0.01)。结论⑴NAs治疗慢性乙型肝炎可导致HBV逆转录酶区变异,NAs抗乙型肝炎病毒效果不佳与HBV逆转录酶区变异密切相关;⑵不同NAs药物导致慢性乙肝患者HBV逆转录酶区变异位点、变异模式特点不同;⑶NAs治疗慢性乙型肝炎的用药种类与方式、治疗时长,患者HBV-DNA水平等对HBV逆转录酶区发生耐药变异均可能产生重要影响。提示在NAs治疗前、治疗过程合适时间点检测NAs耐药相关变异位点和HBV DNA水平,可为临床慢性乙型肝炎的抗病毒治疗提供重要参考。     

热性惊厥患儿T淋巴细胞和红细胞免疫功能的实验研究

目的 探讨小儿热性惊厥的Ｔ淋巴细胞和红细胞免疫功能变化。方法 对７２例热性惊厥患儿及３５例健康儿童应用微量全血氚标记胸腺嘧啶核苷掺入法作Ｔ细胞增殖实验，用酵母花环实验测红细胞免疫丫附功能。结果 热性惊厥患儿外周血Ｔ细胞增殖反应水平明显降低，以复杂性热性惊厥患儿尤为明显，ＣＰＭ和ＳＩ均显著低于健康对照组；热性惊厥患儿红细胞Ｃ３ｂ受体花形成率明显较健康组为低，但红细胞免疫复合物花环形成率则无明显差异。     

补益肝肾法配合美容疗法治疗痤疮75例

目的:观察补益肝肾法配合美容疗法治疗痤疮的临床疗效。方法:将143例痤疮患者随机分为2组。治疗组75例,内服补益肝肾中药(山楂、白花蛇舌草、薏米、旱莲草、女贞子、桑白皮等),同时配合美容疗法;对照组68例,仅用美容疗法。结果:痊愈率治疗组为70.3%,对照组为45.5%;总有效率治疗组8周后为96.0%,对照组为73.5%。2组痊愈率、总有效率比较,差异均有显著性意义(P<0.05)。结论:补益肝肾法配合美容疗法治疗痤疮的疗效优于单纯用美容疗法。     

海口市2010—2020年输入性登革热流行病学特征

Objective This study intends to understand the epidemiological characteristics of imported dengue fever in Haikou city from 2010 to 2020, we provide references for dengue fever prevention and control in Haikou city. Methods The epidemiological investigation and laboratory nucleic acid test data of dengue fever cases in Haikou City from 2010 to 2020 were collected, and analyzed by Excel 2010 and SPSS 20.0 software, to describe the epidemiological characteristics of imported dengue fever cases. Results A total of 72 cases of imported dengue fever were reported in Haikou City from 2010 to 2020, 53 cases were imported from abroad (73.61%) and 19 cases were imported domestically (26.39%), all of which were mild. The number of imported cases from 2017 to 2019 increased by 1 321.68% compared with the average annual imported cases from 2010 to 2016. The cases were concentrated in the main urban area (97.22%), distributed in the streets in the main urban area. The peak of case imported occurred in August-November,in addition, the domestic imported cases were concentrated in September-October. The ratio of male to female was 2.27∶1, and the average age was (34.93 ±1.55) years old. Working, doing business and visiting relatives accounted for 84.22% in China. Overseas workers, business and tourism accounted for 77.78%. There was a significant difference in the reasons for going out between domestic and overseas imported cases (χ2=75.10,P< 0.05). Among the imported sources, Southeast Asia accounted for 94.34% of overseas imported cases, and Guangdong Province accounted for 57.89% of domestic imported cases. The median of onset to diagnosis was 3 days, the first diagnosis rate was 77.78% (56/72), and the screening rate of people who have been infected before entering the country was 28.57%. Most of the dengue viruses infected were type I and type II, accounted for 84.72%. Conclusion The imported cases of dengue fever in Haikou city have gradually changed from scattered occurrence to rapid growth; we should strengthen the publicity and education of dengue fever among migrant workers, businessmen, tourists and relatives in the main urban area of Haikou City, and improve the awareness of disease prevention and medical treatment, strengthen quarantine inspection and medical examination of entry personnel from Southeast Asian countries as well as dengue fever diagnosis and treatment training in grass-roots medical institutions, which can effectively reduce the risk of local transmission caused by imported dengue fever cases. © 2021 Editorial Office of Chinese Journal of Schistosomiasis Control. All right reserved.     

人工晶体植入术后低视力原因分析

目的　分析小切口非超声乳化人工晶体植入术后低视力发生的原因。方法　对 2 18例 (2 4 3眼 )白内障小切口非超声乳化人工晶体植入术患者进行术后 1wk～ 6mo随访 ,观察矫正视力及眼部情况。结果　在术后 6mo2 7例 (34眼 )矫正视力低于 0 .3(13.99% )患者中 ,手术并发症 17眼 (6 .99% ) ,术前原有眼病 13眼 (5 .35 % ) ,两者并存者 4眼 (1.6 5 % )。结论　手术并发症和术前原有眼病是术后低视力发生的原因。减少手术并发症和重视白内障患者术前眼部情况检查 ,是降低白内障摘除人工晶体植入术后低视力发生的两个重要环节。