纤维蛋白对人肝癌细胞系QGY—7703细胞分...

The effects of fibronectin (FN) on cytodifferentiation and cell cycle in a human hepatoma cell line QGY-7703 were studied by flow cytometry, electron microscopy, etc. The results indicated that FN inhibited cell proliferation and mitosis; significant differences were noted between the control group and the experimental group (P < 0.01). FN blocked the cell cycle in phase S; cell spreading improved with less cell overlapping, and the growth of monolayer cell was partially restored. The number of microvilli and marginal ruffles decreased and that of mitochondria, Golgi complex, endoplasmic reticulum increased. The findings suggested that FN could partially restore QGY-7703 cell normal phenotype and probably be useful for the treatment of tumor.     

纤连蛋白对人舌癌T_（56）细胞分化的影响

用离体细胞培养方法及细胞生物学技术，研究纤连蛋白（ＦＮ）对人舌癌细胞株Ｔ＿（５６）的某些生物学效应。结果表明：ＦＮ可抑制细胞增殖和有丝分裂；细胞形态表现出向正常细胞表型特征的逆转；琥珀酸脱氢酶（ＳＤＨ）的显著变化（Ｐ＜０．０１）表明细胞有氧代谢的增强，反映出细胞逆转分化的特征；细胞电泳率值的变化显著（Ｐ＜０．０１），反映细胞膜成分在一定程度上的改变。     

纤连蛋白对人肝癌细胞系QGY-7703细胞分化及细胞周期的影响

用流式细胞光度术等细胞生物学技术,研究纤连蛋白(FN)对人肝癌细胞系QGY-7703细胞分化及细胞周期的影响。结果表明,FN抑制其细胞增殖和有丝分裂,对照组和实验组间差异有显著性(P<0.05);细胞形态向正常细胞逆转,细胞被阻断在S期。作者认为FN可能是一种具有潜在治疗肝癌的药物。     

纤连蛋白作用下Tca8113细胞铺展状况及细胞表面形态的变化

利用细胞培养和光、电镜技术及体视学方法，研究了人舌鳞癌Ｔｃａ８１１３细胞在含不同浓度纤连蛋白（ＦＮ）和／或ＦＮ抗血清的鼠尾胶原基质上细胞铺展面积和片状胞质面积的变化，并观察了细胞表面形态尤其是丝状伪足的改变。结果发现ＦＮ能够改善Ｔｃａ８１１３细胞在胶原基质上的铺展状况。随着基质中ＦＮ含量的提高，细胞形态变扁，发出的丝状伪足增多，细胞向基质中陷入的程度加大，提示ＦＮ可能与肿瘤浸润过程中的某些环节密切相关．