匹罗卡品致(癎)大鼠海马中表达生长抑素的中间神经元数目变化及其轴突出芽

Objective To investigate the roles of somatostatin(SS)positive intemeurons in the development and compensation of temporal lobe epilepsy.Methods Piloearpine-induced epilepsy rat model was established.Immunohistochemistry method was used to detect number changes and axonal sprouting of SS positive intemeurons in different domains of the hippocampus at difierent time points.Degeneration of SS positive interneurons and their neurophils were detected by the double immunofluorescence staining with SS and Fluoro-Jade B(FJB)at 7 and 60 days after status epilepticus (SE).Results In the exoerimental rat group,the number of SS positive neurons decreased in each hippocampal domain,and it reached the lowest at 7 days post-SE(There were 11.1±3.3 in hilus,2.8±0.9 in CA1region and 1.8±0.7 in CA1region,t=13.519,9.644 and 8.808,all P<0.01).In chronic phase,the number of SS neurons gradually recovered,and exceeded the control group in CA1 area at 60 days post-SE(12.8±1.5 vs 8.8±1.3,t=-4.506,P<0.01),however,the number of SS neurons in the hilus(25.5±4.6)and CA1 area(4.8±0.8)remained significantly less than normal levels(t value were 4.691 and 3.953.both P<0.01).Increased SS positive fibers were found in the lacunosum-molecular (1m)layer and outer molecular layer of dentate gyrus after 30 days post-SE,and numerous SS positive fibers were seen threnghout the layers of area CA1 at 60 days post-SE.Double immunofluuorescence revealed that a few SS positive interneurons and fibers were also labeled by FJB in area CA1 at 7 days post-SE and in CA domain/hilus at 60 days post-SE.Conclusions SS intemeurons loss plays an important role in the development of temporal lobe epilepsy.The loss is partially caIlsed by the degeneration and death of neurons;SS positive neurophils increase within area CA1 in chronic phase may play a significant role in the generation and compensation of temporal lobe epilepsy.     

匹罗卡品致(癎)大鼠海马中表达生长抑素的中间神经元数目变化及其轴突出芽

Objective To investigate the roles of somatostatin(SS)positive intemeurons in the development and compensation of temporal lobe epilepsy.Methods Piloearpine-induced epilepsy rat model was established.Immunohistochemistry method was used to detect number changes and axonal sprouting of SS positive intemeurons in different domains of the hippocampus at difierent time points.Degeneration of SS positive interneurons and their neurophils were detected by the double immunofluorescence staining with SS and Fluoro-Jade B(FJB)at 7 and 60 days after status epilepticus (SE).Results In the exoerimental rat group,the number of SS positive neurons decreased in each hippocampal domain,and it reached the lowest at 7 days post-SE(There were 11.1±3.3 in hilus,2.8±0.9 in CA1region and 1.8±0.7 in CA1region,t=13.519,9.644 and 8.808,all P<0.01).In chronic phase,the number of SS neurons gradually recovered,and exceeded the control group in CA1 area at 60 days post-SE(12.8±1.5 vs 8.8±1.3,t=-4.506,P<0.01),however,the number of SS neurons in the hilus(25.5±4.6)and CA1 area(4.8±0.8)remained significantly less than normal levels(t value were 4.691 and 3.953.both P<0.01).Increased SS positive fibers were found in the lacunosum-molecular (1m)layer and outer molecular layer of dentate gyrus after 30 days post-SE,and numerous SS positive fibers were seen threnghout the layers of area CA1 at 60 days post-SE.Double immunofluuorescence revealed that a few SS positive interneurons and fibers were also labeled by FJB in area CA1 at 7 days post-SE and in CA domain/hilus at 60 days post-SE.Conclusions SS intemeurons loss plays an important role in the development of temporal lobe epilepsy.The loss is partially caIlsed by the degeneration and death of neurons;SS positive neurophils increase within area CA1 in chronic phase may play a significant role in the generation and compensation of temporal lobe epilepsy.     

剖宫产同时行子宫肌瘤剔除术65例临床观察

目的探讨子宫下段剖官产同时行子宫肌瘤剔除的可行性。方法选择本院2007年1月-2009年12月65例妊娠合并子宫肌瘤患者为观察组，在剖宫产同时行子宫肌瘤剔除术。抽取同期无任何并发症的正常剖宫产产妇65例作为对照组，观察两组手术时间、术中出血量、术后24h累计阴道出血量、产后出血发生率、术后肛门排气时间、术后病率、子宫复旧情况及住院天数等情况。结果观察组的手术时间和术中出血量多于对照组（P〈0．05），但两组患者术后24h累计阴道出血量、产后出血发生率、术后肛门排气时间、术后病率、子宫复旧情况及住院天数情况比较，差异无统计学意义（P〉0．05）。结论子宫下段剖宫产同时行子宫肌瘤剔除术对于无其他高危因素的产妇可行，但要注意手术技巧。     

抗菌药物专项整治活动对我院胃部及结直肠手术围术期预防用药的影响

目的:比较抗菌药物专项整治活动期间我院胃肠外科胃部手术、结直肠手术在围术期预防用药的合理性,评价抗菌药物专项整治活动对抗菌药物使用的影响。方法:整群选取整治前阶段组(2010年)、整治1阶段组(2011年)和整治2阶段组(2012年)涉及胃部手术、结直肠手术的全部出院病历,进行整治前后预防用抗菌药物合理性的对照研究,考察整治措施的有效性和可行性。结果:预防用抗菌药物综合不合理率整治2阶段为74.92%,较整治前阶段的96.40%明显降低(P<0.05)。整治1阶段初次预防给药时机较整治前明显改善(P<0.05);整治2阶段组除初次给药时机的合理率明显提高(P<0.05)外,用药疗程、术中追加、联合用药等指标合理率也明显提高(P<0.05)。整治各阶段手术部位感染率差异无统计学意义(P>0.05)。结论:专项整治活动的开展和整治干预措施的出台对胃肠外科胃部及结直肠手术围术期抗菌药物的使用产生了一定影响,尤其是强有力的奖惩措施、强度指标细化控制、开展前瞻性研究等措施更是成效显著。     

陪护掌握中风偏瘫患者良肢位知识现状调查分析

目的了解陪护掌握中风偏瘫患者良肢位知识现状，为完善陪护专科知识培训及考核方法提供依据。方法采用问卷调查法对60名陪护生进行了掌握中风偏瘫患者良肢位知识现状调查。结果仅有6名（10％）了解良知位摆放的目的及意义，有28名（46．7％）掌握良知位的摆放方法，有15名（25％）未听说过良知位。结论陪护人员进行掌握中风偏瘫患者良肢位知识不足，需加强并规范陪护人员专科专病相关知识的培训。     

妇科恶性肿瘤化疗24例护理体会

女性生殖系统肿瘤约占全身肿瘤的1/5,可发生在生殖器官各部[1]。目前,多无早期诊断妇科恶性肿瘤的可靠方法,特别是卵巢恶性肿瘤,以致临床上发现的妇科恶性肿瘤多属晚期。为了延长生命及提高生存质量,约一半以上的恶性肿瘤患者需进行化疗,力求巩固疗效,增加治愈率。化疗是控制肿瘤发展、延长患者寿命的重要方法,但化疗产生的毒副反应也常常给患者带来痛苦。许多患者在接受化疗期间会出现多种生理及心理方面的反应和需求。对恶性肿瘤患者化疗前后如能进行细致的临床观察,及时掌握患者的心理特征及化疗的各种不良反应,给予患者精心的护理,不但…     

盐酸左氧氟沙星眼用凝胶对家兔眼刺激性试验

目的：观察家兔眼接触盐酸左氧氟沙星眼用凝胶后产生的刺激反应情况和恢复情况。方法：给家兔右眼滴用左氧氟沙星眼用凝胶，每天2次，连续7天后继续观察7天角膜、虹膜及结膜的反应情况和恢复情况。结果：家兔角膜、虹膜在各观察时间的刺激反应平均分值均为0，结膜有轻微反应，家兔眼刺激的综合平均分值在0～3．9范围内。结论：盐酸左氧氟沙星眼用凝胶对家兔眼无明显刺激反应．符合眼刺激试验要求。     

辛伐他汀对急性脑梗死患者血浆同型半胱氨酸和血脂的影响

目的：观察辛伐他汀对急性脑梗死患者血浆同型中胱氩酸（Hcy）和血脂的影响。方法：发病7天内脑梗死患者60例，随机分为治疗组和对照组（各30例），治疗组采用辛伐他汀20mg/d，其6周；对照组则不用调脂药，其它治疗相同。治疗前后分别检测血浆Hcy浓度、血脂水平和进行神经功能缺损评分，并进行两组比较。结果：辛伐他汀治疗后总有效率（90．00％）明显高于对照组（40．00％）（P〈0．05），血脂水平明显改善，差异有显著性；血浆Hcy浓度稍降低，而对照组不明监。结论：辛伐他汀可降低血脂水平，有效治疗脑梗死，但对Hcy作用不明显。