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Objective To investigate the effects of diets with different calorie and nutritional values on pubertal onset in female rats. Method Female Wistar rat models receiving calorie-deprived (group R) (Experiment 1) ,fat-rich(group F) ,glucose-rich(group G) and protein-rich(group P) (Experiment 2) test diets were established,and rats receiving diets with normal caloric value were considered as control group (group C). The body weight (BW) ,food-intake and vaginal patency (VP) were observed. The rats were killed at the day of vaginal patency. The BW, uterus weight (UW), uterus index (UI), calorie intake per day (CI), perirenal fat weight (PFW) and perirenal fat index (PFI) were measured. The serum levels of leptin, ghrelin, IGF- Ⅰ were tested by means of ELISA.Results Experiment 1:The VP was retarded in group R compared with group C (P<0. 01). There was a significant difference in BW, UW,UI ,CI,leptin and ghrelin at the VP day between group R and group C (all P<0.01) ,and no significant difference in IGF- Ⅰ levels between these two groups. Experiment 2 :The VP was retarded in group F,group G and group P in comparison with group C (P<0.01). The BW, PFW and PFI in group G and group F were markedly higher than those in group P and group C. The leptin level in group F was the highest, and the lowest in group P. The ghrelin level in group G was the highest,and that in group P was the lowest. However,there were no significant differences in IGF- Ⅰ and UI among these, four groups. Conclusion The normal pubertal onset of female rats requires sufficient caloric stores and balanced nutrient. Diets malnutrition and fat-rich, glucoserich and protein-rich test diets all delay pubertal onset in female rats. 相似文献
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Objective To investigate the effects of diets with different calorie and nutritional values on pubertal onset in female rats. Method Female Wistar rat models receiving calorie-deprived (group R) (Experiment 1) ,fat-rich(group F) ,glucose-rich(group G) and protein-rich(group P) (Experiment 2) test diets were established,and rats receiving diets with normal caloric value were considered as control group (group C). The body weight (BW) ,food-intake and vaginal patency (VP) were observed. The rats were killed at the day of vaginal patency. The BW, uterus weight (UW), uterus index (UI), calorie intake per day (CI), perirenal fat weight (PFW) and perirenal fat index (PFI) were measured. The serum levels of leptin, ghrelin, IGF- Ⅰ were tested by means of ELISA.Results Experiment 1:The VP was retarded in group R compared with group C (P<0. 01). There was a significant difference in BW, UW,UI ,CI,leptin and ghrelin at the VP day between group R and group C (all P<0.01) ,and no significant difference in IGF- Ⅰ levels between these two groups. Experiment 2 :The VP was retarded in group F,group G and group P in comparison with group C (P<0.01). The BW, PFW and PFI in group G and group F were markedly higher than those in group P and group C. The leptin level in group F was the highest, and the lowest in group P. The ghrelin level in group G was the highest,and that in group P was the lowest. However,there were no significant differences in IGF- Ⅰ and UI among these, four groups. Conclusion The normal pubertal onset of female rats requires sufficient caloric stores and balanced nutrient. Diets malnutrition and fat-rich, glucoserich and protein-rich test diets all delay pubertal onset in female rats. 相似文献
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阴茎硬结症是一种临床上较为少见的男性疾病,因其起病隐秘,病症复杂,病程缠绵且容易复发,同时伴阴茎疼痛、勃起障碍等症状,对男性正常的工作生活造成较大影响。现代医学对于阴茎硬结症尚无有效治疗手段,作者通过长期临床观察,认为阴茎硬结症属于中医络病范畴,以络病学说“易滞易瘀”的病理特点为基础,将“阳虚络瘀”视作阴茎硬结症的基本病机,运用“温阳通络”法治疗阴茎硬结症验案多例,疗效颇著。 相似文献
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有类似POEMS综合征表现的Castleman病一例 总被引:1,自引:0,他引:1
患者,男,70岁。2005年7月起逐渐出现手背、足踝处皮肤色素沉着,四肢乏力,感觉减退等症状。2005年9月症状加重,伴有头昏,双下肢凹陷性水肿,入住我院神经内科,诊断为:“椎基底动脉供血不足,POEMS综合征”。患者拒绝作骨髓穿刺和淋巴结活检,经积极对症支持治疗后好转出院。2006年1月,患者出现腹胀、胸闷、呼吸急促,活动后加重,伴有纳差、尿少、双下肢水肿。外院作颈部淋巴结活检,示反应性淋巴结炎,经对症治疗效果不明显,再次转入我院。起病以来,患者无发热、盗汗。 相似文献
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uPASNVEGF在脑膜瘤、胶质瘤、脑转移癌中的表达及意义 总被引:1,自引:0,他引:1
背景与目的:肿瘤的生长须依赖新生血管的生成,而目前认为血管内皮生长因子(vascular endothelial growth factar,VEGF)、尿激酶型纤溶酶原激活物(urokinase—type plasminogen activator,uPA)是刺激肿瘤血管形成和肿瘤浸润过程中的重要因子.本文探讨VEGF和uPA在脑膜瘤、胶质瘤、脑转移癌中的表达及临床意义。方法:应用免疫组化法检测30例脑膜瘤、30例脑转移癌、40例胶质瘤及10例正常脑组织中uPA和VEGF的表达,并结合临床资料分析。结果:vEGF与uPA在三种肿瘤中均有表达;VEGF和uPA表达与瘤周水肿程度呈正相关;VEGF和uPA表达与胶质瘤恶性程度呈正相关。结论:检测vEGF和uPA对脑膜瘤、胶质瘤、脑转移癌的生物学行为、瘤周水肿和预后评估有参考价值。 相似文献
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目的:探讨机器人辅助腹腔镜下肾盂成形术(robot-assisted laparoscopic pyeloplasty, RALP)治疗儿童肾盂输尿管连接部梗阻(ureteropelvic junction obstruction, UPJO)的临床应用效果。方法:回顾性分析2021年9月—2022年5月在宁夏医科大学总医院接受RALP的31例UPJO患儿,其中男22例,女9例;年龄(57.35±47.10)个月;左侧23例,右侧8例;体重(20.77±12.20) kg。收集手术时间、术中估计出血量、术后引流时间、术后住院时间等临床资料,肾盂前后径(anteroposterior diameter, APD)、肾盂与肾实质厚度比值(pelvis/cortex ratio, PCR)、分肾功能(differential renal function, DRF)等影像学资料。采用第四代DaVinci Xi机器人操作系统,重建肾盂输尿管连接部。结果:31例患儿手术均顺利完成,无中转开放手术病例。平均手术时间为(119.87±15.64) min,平均肾盂输尿管吻合时间(33.65±7.45... 相似文献
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目的:探讨建立较为简便的新生大鼠皮层神经元细胞体外无血清原代培养方法。方法取新生大鼠(24 h )大脑皮层组织,消化后种植在有多聚-L-赖氨酸包被的六孔培养板中,以含10%胎牛血清DEM E-HG培养液种植,4~8 h后换用含B27的Neurobasal培养基维持饲养。于不同时间在倒置相差显微镜下观察形态变化,分别采用RT-PCR、Western blot、免疫组织化学对神经元特异性标记物神经元特异性烯醇化酶(NSE)基因及蛋白进行鉴定。结果2~8 h神经元细胞贴壁,随着时间延长,形态多变,突起逐渐增多,神经元突起间相互接触形成网络,培养7~10 d神经元胞体最为丰满,通过RT-PCR、Western blot和免疫组织化学证明所分离培养的是神经元细胞。结论该方法简单易行,神经元纯度较高,可作为神经元体外培养的良好模型用于以后的研究。 相似文献
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目的探讨内镜联合腹腔镜手术治疗胃肠道肿瘤的适应范围、安全性和可行性。方法对我院2007年9月—2010年7月进行的30例内镜指导下腹腔镜手术治疗胃肠道肿瘤的临床资料进行回顾性分析,其中胃间质瘤2例,结肠腺瘤20例,早期结肠腺瘤局灶性癌变8例。结果 30例手术均顺利完成,手术定位准确,无中转开腹者。手术时间40~90min,术中出血量10~40mL,胃肠功能恢复时间1~2d。术后未发生大出血、吻合口漏、切口感染或腹腔内感染、尿潴留等并发症。住院时间4~6d。术后随访6~24个月均未见复发。结论内镜指导下腹腔镜手术对于内镜下难以切除,而腹腔镜下又定位困难的胃肠道肿瘤,具有定位准确、创伤小、并发症少、术后恢复快等特点,为胃肠道小肿瘤的微创治疗提供了一种新的思路和合理的治疗方案,值得临床推广应用。 相似文献
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目的:探讨盆底功能失调型便秘的生物反馈治疗的效果。方法:回顾性分析46例盆底功能失调型便秘行生物反馈治疗患者的资料。结果:44例坚持完成治疗的患者中,35例肛管直肠压力测定示直肠感觉阈值、最大耐受容量、直肠肛门抑制反射较治疗前降低,盆底肌的反常收缩消失,恢复正常排便;6例排便次数,排便不尽感,肛门坠胀感较治疗前有所改善;3例无效。2例终止治疗后失访。总有效率为93.2 %。结论:盆底功能失调型便秘生物反馈治疗近期疗效满意,具有无创、费用低、无需住院等特点。 相似文献