细胞因子治疗4.5Gyγ射线照射比格犬造血损伤的机制初探

目的 探讨多种细胞因子配伍应用治疗4.5 Gy γ射线照射比格犬造血系统损伤的作用及可能机制。方法 16只比格犬均给予4.5 Gy ⁶⁰Co γ射线全身照射,随机分为照射对照、综合对症和细胞因子3组。细胞因子组在综合对症支持治疗的基础上应用rhG-CSF、rhIL-11和rhIL-2联合治疗,采用流式细胞术检测外周血中CD34+细胞含量、有核细胞周期及凋亡比例。结果 4.5 Gy γ射线照射犬外周血中CD34+细胞含量在照射后1 d明显下降(照射对照组和综合对症组分别为照前值的61.3%和52.1%),G₀/G₁期有核细胞比例增加(分别为99.27%和99.49%),且凋亡率(分别为26.93%和21.29%)和坏死率(分别为3.27%和4.14%)明显升高(与照前值比较, P<0.05);而经过细胞因子治疗后,外周血中CD34+细胞含量在照射后1 d即明显升高(为照前值的135.6%),G₀/G₁期有核细胞比例(99.71%)进一步增加,其凋亡率(5.66%)和坏死率(1.60%)明显低于照射对照和综合对症组。结论 本研究的细胞因子组合可能通过动员骨髓中CD34+细胞到外周血,使细胞周期阻滞在G₀/G₁期,减少细胞凋亡,从而促进极重度骨髓型急性放射病犬造血功能的恢复。     

重楼甾体总皂苷对血小板聚集的直接诱导作用及初步机制研究

目的:确定重楼甾体总皂苷(TSSP)体外直接诱导血小板聚集作用并对其可能的机制进行探索.方法:比浊法测定血小板聚集,透射电镜观察血小板形态学改变.结果:体内外血小板聚集模型研究表明,TSSP体内给药能够增强ADP诱导血小板聚集.体外能够直接诱导血小板聚集,并呈剂量效应关系.电镜观察TSSP能够直接激活血小板引起变形释放等反应.肾上腺素能够增强TSSP诱导的血小板聚集,该增强作用能被酚妥拉明所拮抗.蛋白酪氨酸磷酸酶抑制剂过钒酸钠能够增强TSSP诱导血小板聚集.结论:本研究表明,TSSP中含有参与止血作用的药理活性成分.     

加味四君子汤对脾虚大鼠免疫功能、血液流变性恢复的影响

用应激过劳和饥饱法造成脾虚证模型,大鼠的免疫功能明显下降,全血粘度低切率明显降低,红细胞聚积指数明显下降,胸腺和脾脏明显萎缩,加味四君子汤能明显促进上述改变恢复正常。     

Therapeutic effects of combined cytokines on hematopoietic injuries induced by 4.5 Gy γ-rays irradiation in beagles

Objectivc To observe the therapeutic effects of combined cytokines on hematopoietic injuries induced by 4.5 Gy60 Co γ-rays irradiation in beagles,and to provide experimental evidences for the clinical treatment of extremely severe myeloid acute radiation sickness(ARS).Methods 16 beagles were given 4.5 Gy60 Co γ-rays total body irradiation,and then randomly assigned into irradiation control group,supportive care group and cytokines group.In addition to supportive care,recombinant human granulocyte colony-stimulating factor (rhG-CSF),recombinant human interleukin-11(rhIL-11)and recombinant human interleukin-2(rhIL-2)were administered subcutaneouly to dogs in cytokines group.Peripheral blood hemogram was examined once every two days.Bone marrow and peripheral blood were collected to proceed colony cultivation 4 d pre-irradiation and 1 and 45 d post-irradiation.Conventional histopathological sections of sternum were prepared to observe the histomorphology changes. Results After irradiation,the population of all kinds of cells in peripheral blood declined sharply.WBC nadir Was elevated(1.04×109/L,but 0.28×109/L and 0.68×109/L for the irradiation control group and the supportive care group separately),the duration of thrombocytopenia was shortened (24 days,but 33 days for the supportive care groug) and red blood cell counts were maintained in the range of normal values after cytokincs treatment in combination.The colony forming efficiency of haemopoietic stem cells(HSCs)in bone marrow and peripheral blood decreased obviously 1 d post irradiation,but recovered to the level of that before irradiation 45 d post irradiation after supportive care and cytokines treatment.Hematopoietic cells disappeared in bone marrow of animals in irradiation control group,but hematopoietic functions were recovered after cytokines were administrated.Conclusions RhG-CSF.rhIL-11 and rhIL-2 used in combination could elevate WBC nadir,accelerate the recovery of leukocytes,platelets and red blood cells and promote the proliferation,differentiation and maturity of HSPCs left in the body after 4.5 Gy γ-rays total body irradiation,eventually restore the hematopoietic function.Hence,combination of rhG-CSF,rhIL-11 and rhIL-2 could serve as better therapeutic strategy to treat extremely severe myeloid ARS.     

Experimental therapeutic effect of combined cytokines on beagle dogs exposed to 4.5 Gy γ-rays

Objective To evaluate the effects of combined administration of recombinant human interleukin-11(rhIL-11),recombinant human G-CSF(rhG-CSF)and recombinant human interleukin-2 (rhIL-2)on acute radiation sickness(ARS)beagles.Methods Sixteen beagle were irradiated with 4.5 Gy60 Co γ-rays to establish ARS models,and were divided into irradiation control group,supportive care group and combined cytokines treatment group.After irradiation irradiation control group was given no treatment,the dogs in supportive care group received purely symptomatic treatment,while combined cytokines treatment group received rhIL-11 50μg/(kg·d)and rbG-CSF 10μg/(kg·d)subcutaneously(0-14 d)and rhIL-2 1×1 06 U/d(29-43 d)besides symptomatic treatment.Manifestation and characteristics of ARS beagles were observed,and the survival time were recorded.At last,post-mortem examination and histological examination were performed.Results All animals underwent nausea,diarrhea and fever.After irradiation,all animals in irradiation control group died in two weeks,and the mean survival time was 12.7 d,while only one died at 33 d in supportive care group.All dogs in combined cytokine group survived at 45 day after exposure,and their haematopoiesis and gastrointestinal tract were recovered.Conclusions Combination of rhIL-11 + rhG-CSF + rhIL-2 treatment could be significantly effective on ARS beagles irradiated by 4.5 Gy60 Co γ-rays,which could accelerate injured haemotopoiesis and intestinal tract recovery,increase the survival rate and improve the life quality of animals.     

Clotting mechanism in beagles irradiated by 4.5 Gy γ-rays

Objective To explore the clotting mechanism in beagles irradiated by 4.5 Gy γ-rays after treatment with supportive care,or supportive care and combined cytokines.Methods Sixteen beagles were divided into irradiation control group,Supportive care group and combined cytokines treatment group.Platelet aggregation test,thrombelagtography (TEG) and the time measurement were analyzed in vitro.Results In irradiation group and supportive care group,the platelet aggregation rates in beagles were decreased markedly and the k value of TEG was increased 7 d post-irradiation,while those indexes in combined cytokines treatment group changed little.At 14 d post-irradiation,each parameter of TEG in irradiated group changed obviously.The values of r,k,r+k and M were elevated significantly,clotting time and the maximum coagulation time of thrombus delayed,the Ma value was decreased markedly,and the maximum elasticity amplitude of thrombus was diminished.All parameters in combined cytokines treatment group were better than those in supportive care group.The thrombin time was prolonged obviously in irradiated group 14 d post-irradiation,while the thrombin time was the longest at 2-3 weeks post irradiation in supportive care group and combined cytokines treatment group(P>0.05).Conclusions Cytokines could improve the platelet aggregation and the blood clotting functions of beagles suffering from acute radiation sickness.     

中药保留灌肠在急性阑尾炎中的应用临床观察

目的探讨中药保留灌肠治疗急性阑尾炎的临床效果。方法将我院收治的64例急性阑尾炎患者,随机分为对照组和观察组,对照组采用西医对症支持治疗,观察组采用中药保留灌肠治疗。分析两组治疗效果及治疗过程中不良反应发生情况。结果观察组显效率、总有效率显著高于对照组,差异有统计学意义（P〈0.05）。观察组肛门排气时间、肛门排便时间、肠鸣音恢复时间显著早于对照组,差异有统计学意义（P〈0.05）。观察组随访期间复发率显著低于对照组,差异有统计学意义（P〈0.05）。结论中药保留灌肠治疗急性阑尾炎疗效优于西医对症支持治疗,患者治疗后胃肠道功能恢复良好,复发率低,使用方便,值得临床推广应用。     

中医挂线配合中药熏洗治疗复杂性肛瘘60例临床分析

目的:探讨中医挂线配合中药熏洗治疗复杂性肛瘘的临床效果.方法:将我院收治的60例复杂性肛瘘患者,随机分为对照组和观察组,对照组采用切缝挂线手术治疗,观察组在对照组的基础上给予中药熏洗治疗.分析两组的治疗效果、创口愈合情况、并发症发生情况等.结果:观察组显效率及总有效率显著高于对照组,差异有统计学意义(P＜0.05).观察组创面平均愈合时间显著短于对照组,差异有统计学意义(P＜0.05).观察组复发率显著低于对照组,差异有统计学意义(P＜0.05).结论:采用中医挂线联合中药熏洗治疗复杂性肛瘘,疗效优于单用中医挂线治疗,创面愈合时间快,复发率低,使用方便,值得临床推广应用.     

强制隔离戒毒人员应对方式特点分析与戒毒矫治对策

目的:分析强制隔离戒毒人员的应对方式特点,提出戒毒矫治对策。方法:对86例强制隔离戒毒人员进行一般情况调查和应对方式测试,用SPSS 11.5统计软件进行统计。结果:不同职业状况(P<0.05)、不同文化程度(P<0.01)和不同家庭氛围(P<0.05)的强制隔离戒毒人员在积极应对上存在显著性差异;参加不同强戒次数的强制隔离戒毒人员在消极应对上存在显著性差异,P<0.01。结论:职业状况、家庭氛围、文化程度、强戒次数对强制隔离戒毒人员的应对方式有影响。建议根据强制隔离戒毒人员的应对方式特点,开展戒毒矫治工作。     

重组人干细胞因子对猕猴外周血干细胞的动员作用

给正常成年猕猴每天一次皮下注射重组人粒细胞集落刺激因子 10 μg·kg-1·d-1(μKD)或 (和 )重组人干细胞因子 2 0、5 0和12 5 μKD ,前者给药 5天 ,后者 8天 ,观察国产重组人干细胞因子动员外周血干细胞的效果。结果表明 ,给药后外周血白细胞数最高值rhG CSF单独给药组为 2 0 6 % ,rhSCF单独给药为 2 0 0 % ,而两者联合给药后为 2 80 %～ 310 % ;外周血CFU GM较动员前分别升高 1 1、1 3和 5 8～7 9倍 ;3个联合动员组外周血CD34+ 细胞数分别为动员前的 2 0、2 4和 35倍。说明rhSCF对外周血干 /祖细胞有明显的动员作用 ,rhSCF+rhG CSF联合动员效果更佳