Correction to: Effect of JAK inhibitors on high- and low-density lipoprotein in patients with rheumatoid arthritis: a systematic review and network meta-analysis

Clinical Rheumatology -     

Bioinformatics Analyses of Potential miRNA-mRNA Regulatory Axis in HBV-related Hepatocellular Carcinoma

Aims: We aimed to explore the crucial miRNA-mRNA axis through bioinformatics analysis and provide evidences for the development of pathophysiological mechanisms and new therapies for HBV-related HCC.Methods: MiRNA ({"type":"entrez-geo","attrs":{"text":"GSE76903","term_id":"76903"}}GSE76903) and mRNA ({"type":"entrez-geo","attrs":{"text":"GSE77509","term_id":"77509"}}GSE77509) dataset were used to screen differentially expressed miRNAs (DE-miRNAs) and differentially expressed mRNAs (DE-mRNAs) using R software. Overlapping genes between DE-mRNAs and target genes of DE-miRNAs were identified as candidate genes. Hub genes were obtained via cytohubba analysis. The expression at protein and mRNA levels and prognostic value of hub genes were evaluated based on The Cancer Genome Atlas (TCGA) data. Key miRNA-mRNA axes were constructed according to predicted miRNA-mRNA pairs. MiRNA expression and prognostic role were respectively identified using starBase v3.0 and Kaplan-Meier plotter database. Real-time PCR was performed to verify the expression of crucial miRNAs and mRNAs. Coexpression of crucial miRNA and mRNA were analyzed using starBase v3.0.Results: CDK1, CCNB1, CKS2 and CCNE1 were screened as hub genes, which were significantly upregulated at protein and mRNA levels. These up-regulated hub genes were also significantly associated with poor prognosis. Hsa-mir-195-5p/CDK1, hsa-mir-5589-3p/CCNB1 and hsa-let-7c-3p/CKS2 were screened as critical miRNA-mRNA axes. Critical miRNAs were decreased in HCC, which indicates unfavourable prognosis. QPCR results showed that crucial miRNAs were decreased, whereas critical mRNAs were increased in HBV-related HCC. A reverse relationship between miRNA and mRNA in crucial axis was further verified.Conclusion: This study identified several miRNA-mRNA axes in HBV-related HCC. Hsa-mir-195-5p/CDK1, hsa-mir-5589-3p/CCNB1 and hsa-let-7c-3p/CKS2 might serve as potential prognostic biomarkers and therapeutic targets for HBV-related HCC.     

NAc Shell Arc/Arg3.1 Protein Mediates Reconsolidation of Morphine CPP by Increased GluR1 Cell Surface Expression: Activation of ERK-Coupled CREB is Required

Background:

Relapse into drug abuse evoked by reexposure to the drug-associated context has been a primary problem in the treatment of drug addiction. Disrupting the reconsolidation of drug-related context memory would therefore limit the relapse susceptibility.

Methods:

Morphine conditioned place preference (CPP) was used to assess activity-regulated cytoskeleton-associated protein (Arc/Arg3.1) and correlative molecule expression in the Nucleus accumbens (NAc) shell during the reconsolidation of morphine CPP. U0126 and Arc/Arg3.1 antisense oligodeoxynucleotide were adapted to evaluate the role and the underlying mechanism of Arc/Arg3.1 during the reconsolidation.

Results:

The retrieval of morphine CPP in rats specifically increased the Arc/Arg3.1 protein level in the NAc shell, accompanied simultaneously by increases in the phosphorylation of extracellular signal-regulated kinase1/2 (pERK1/2), the phosphorylation of Cyclic Adenosine monophosphate (cAMP) response element-binding (pCREB), and the up-regulation of the membrane α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors GluR1 subunit level. Intra-NAc shell infusion U0126, an inhibitor of the Mitogen-activated protein kinase kinase (MEK), prevented the retrieval-induced up-regulation of pERK1/2, pCREB, Arc/Arg3.1, and membrane GluR1 immediately after retrieval of morphine CPP. The effect of disrupting the reconsolidation of morphine CPP by U0126 could last for at least 14 days, and could not be evoked by a priming injection of morphine. Furthermore, the specific knockdown of Arc/Arg3.1 in the NAc shell decreased the membrane GluR1 level, and impaired both the reconsolidation and the reinstatement of morphine CPP.

Conclusions:

Arc/Arg3.1 in the NAc shell mediates the reconsolidation of morphine-associated context memory via up-regulating the level of membrane of GluR1, for which the local activation of the ERK-CREB signal pathway, as an upstream mechanism of Arc/Arg3.1, is required.     

调脂治疗是收益还是风险？

诸多的研究结果证明了他汀类药物调脂治疗对降低心血管事件具有一定的有效性和安全性。然而,也有研究结果显示了强化调脂治疗的风险性。为尽可能发挥调脂治疗的功效又避免或减少用药的风险,可遵循生活方式干预、常规剂量给药、全面调脂治疗、个体化治疗和综合治疗等原则。     

L-精氨酸对糖尿病大鼠肾脏、大脑、睾丸氧自由基和抗氧化水平的影响

目的探讨L-精氨酸对糖尿病(DM)大鼠肾脏、大脑、睾丸氧自由基和抗氧化水平的影响.方法给大鼠腹腔注射四氧嘧啶制备DM模型,随机分为DM组、L-精氨酸治疗组及正常对照组;用药4 w末处死大鼠,测定肾脏、大脑及睾丸组织一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及一氧化氮(NO)、丙二醛(MDA)含量.结果 DM大鼠睾丸NOS活性(P＜0.01)及大脑、睾丸NO含量(P＜0.05,P＜0.001)、肾脏SOD(P＜0.001)、肾脏、大脑、睾丸GSH-Px活性(P＜0.01,P＜0.001,P＜0.05)均较正常对照组显著降低,而肾脏、大脑、睾丸MDA含量均较正常对照组明显升高(均P＜0.001);L-Arg可显著增加DM大鼠肾脏、大脑、睾丸NOS活性和NO含量(均P＜0.001)及大脑SOD(P＜0.01)、大脑、睾丸GSH-Px活性(P＜0.05,P＜0.001),并使肾脏、大脑、睾丸MDA含量显著降低(分别P＜0.01,P＜0.001,P＜0.001).结论 DM大鼠肾脏、大脑、睾丸组织存在脂质过氧化损伤;L-Arg通过提高NO含量,保护抗氧化酶活性,对DM大鼠肾脏、大脑、睾丸组织的氧化应激有一定的减轻作用.     

男女大学生卡特尔16种人格因素量表测查结果比较的meta分析

目的:了解男女大学生的16种人格因素是否存在差异及其相关因素。方法:采用meta分析对2000-2009年间的21篇研究(使用工具均为卡特尔16种人格因素量表),共计22组数据进行统计分析。结果:女生在乐群性、聪慧性、兴奋性、敏感性、幻想性、世故性、忧虑性上的得分均高于男生,平均效果量在-0.41～-0.08之间(均P<0.01);男生在稳定性、有恒性上的得分高于女生,平均效果量分别为0.19和0.08(均P<0.001)。在多水平模型中,乐群性、独立性、紧张性不同年龄段大学生得分不同的影响(β1=0.05,-0.04,0.04;均P<0.05);稳定性、敢为性出版年代的影响(β1=0.04,0.05;均P<0.01);兴奋性不同量表版本的影响(β1=0.07,P<0.05);实验性不同样本量的影响(β1=0.001,P<0.05);实验性、紧张性受出版质量的影响(β1=0.13,-0.11;均P<0.05);独立性不同来源地区的影响(β1=-0.15,P<0.001)。结论:男女大学生在9种人格因素上存在差异,并与样本年龄段、量表出版年代、量表版本、样本量、量表出版质量、样本来源地区因素相关。     

Clara细胞分泌蛋白10在5岁以下喘息儿童外周血中的表达

目的：探讨外周血中Clara细胞分泌蛋白10（CC10）和血清总IgE浓度在5岁以下喘息儿童中的表达。方法：随机选取5岁以下反复喘息患儿59例,分为有特应质高危因素的喘息Ⅰ组（n=33）和无特应质高危因素的喘息Ⅱ组（n=26）,对照组为近期无感染疾病史的外科术前患儿(n=23)。采用固相夹心酶免疫吸附实验（ELISA）测定3组患儿外周血CC10与IgE水平。结果：喘息Ⅰ组、Ⅱ组CC10水平(3.95±1.26, 5.41±1.64 ng/mL)均低于对照组(8.72±2.23 ng/mL),差异有统计学意义(P<0.01);喘息Ⅰ组CC10水平低于喘息Ⅱ组(P＜0.05)。喘息Ⅰ组IgE水平高于喘息Ⅱ组和对照组,差异有统计学意义（P＜0.05）,喘息Ⅱ组和对照组之间差异无统计学意义（P＞0.05）。喘息Ⅰ组血清CC10与IgE呈负相关（r=-0.912, P＜0.01）。结论：外周血CC10水平在5岁以下患儿喘息发作期显著降低,有特应质高危因素的患儿降低更为明显,并与外周血IgE水平呈负相关。     

婴幼儿常见下呼吸道感染性疾病与淋巴细胞亚群变化关系的研究

目的 探讨淋巴细胞亚群在儿童常见下呼吸道感染支气管炎、支气管肺炎和毛细支气管炎中的变化及临床意义。方法 选取111 例支气管炎、418 例支气管肺炎和83 例毛细支气管炎患儿为疾病组,同期健康婴幼儿235 例为对照组,用流式细胞仪检测各组淋巴细胞亚群。结果 支气管炎组总T 淋巴细胞、CD3⁺CD8⁺细胞低于对照组(P<0.05)。支气管肺炎组总T 淋巴细胞和CD3⁺CD8⁺ 细胞低于对照组、Th 和CD4/CD8 高于对照组,且Th 比例高于支气管炎组;与轻症肺炎组相比,重症肺炎组总T 淋巴细胞降低而B 淋巴细胞升高(P<0.05)。毛细支气管炎组Th 细胞和CD4/CD8 高于对照组、CD3⁺CD8⁺ 细胞低于对照组(P<0.01)。与对照组比,3 组下呼吸道感染患儿的B 淋巴细胞增高、NK 细胞比例降低(P<0.05)。结论 细胞免疫功能紊乱或低下以及体液免疫功能亢进参与了婴幼儿下呼吸道感染的发生和发展,并且变化程度与疾病类型及病情程度有关。     

Giant liposarcoma of the esophagus: A case report

Liposarcomas rarely develop in the aerodigestive tract.Here,we present a primary esophageal liposarcoma that was discovered between the T3 and T7 levels of the esophagus during right pleural exploration of a 51-year-old male patient.The patient had presented with non-specific symptoms,including progressive dysphagia over the previous 6 mo,without complaints of chest or epigastric pain,regurgitation,or weight loss.A radical three-hole esophagectomy was performed.The tumor was extremely large(14 cm × 7.0 cm × 6.5 cm),but completely encapsulated.Upon histological examination,the tumor was diagnosed as a giant,well-differentiated esophageal liposarcoma with a dedifferentiated component.Non-specific radiological and endoscopic results during the clinical work-up delayed diagnosis until post-operative histology was performed.In this report,the clinical,radiological and endoscopic diagnostic challenges specific to the case are discussed,as well as the surgical and pathological findings.