全文获取类型
收费全文 | 202篇 |
免费 | 2篇 |
国内免费 | 29篇 |
学科分类
医药卫生 | 233篇 |
出版年
2019年 | 1篇 |
2016年 | 4篇 |
2015年 | 4篇 |
2014年 | 7篇 |
2013年 | 7篇 |
2012年 | 15篇 |
2011年 | 25篇 |
2010年 | 18篇 |
2009年 | 22篇 |
2008年 | 13篇 |
2007年 | 16篇 |
2006年 | 7篇 |
2005年 | 6篇 |
2004年 | 9篇 |
2003年 | 13篇 |
2002年 | 7篇 |
2001年 | 11篇 |
2000年 | 3篇 |
1999年 | 9篇 |
1998年 | 3篇 |
1997年 | 5篇 |
1996年 | 1篇 |
1995年 | 6篇 |
1994年 | 5篇 |
1992年 | 2篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1983年 | 2篇 |
1981年 | 2篇 |
排序方式: 共有233条查询结果,搜索用时 991 毫秒
1.
2.
3.
4.
Objective To explore the effect of Fufangkushen on gastric cancer cell killing by human γδT cells. Methods Isopentenyl pyrophosphate method was used to amplify human peripheral blood γδT cells in vitro. Fufangkushen at various concentrations was used to induce γδT cells and gastric cancer cell lines SGC-7901 for 24 hours, MTr assays was used to detect inhibitory effect of Fufangkushen on these cell lines, LDH assays was used to measure the cytotoxic activity of γδT cells, and flow cytometry was used to detect apoptosis of γδT cells and SGC-7901 before and after the treatment. Results Ten days after cultivation, proliferation ra-tio of γδT cells increased from 4.21% to 70.35% and CD44 was up to 94.0%. Inhibitory rate of Fufangkush-en on SGC-7901 at various concentrations was significantly higher than that on γδT cells (22.3% vs-22.4%, P<0.05). The negative inhibitory ratio on γδT cells showed a dose-dependent manner with Fufangkushen's concentrations ranging from 1/5 to 1/400. γδT cells cytotoxic activity to SGC-7901 induced by Fufangkushen for 24 h was higher than control group, (83.6% vs 71.2%, P<0.05). Apoptotic rate was significantly lower in γδT cells than in SGC-7901 (4.64% vs49.23%, P<0.05). Conclusion Fufangkushen, within routine concentration ranges, can promote γδT cells' proliferation, inhibit tumor cell growth and enhance γδT cells' cytotoxic activity. This may be beneficial to tumor adoptive immunotherapy and provide evidence for the appli-cation of Fufangkushen in the treatment of tumors. 相似文献
5.
阿司匹林对人γδT细胞杀伤消化系统肿瘤细胞的影响 总被引:1,自引:2,他引:1
目的:探讨阿司匹林对人γδT细胞杀伤消化道肿瘤细胞株的影响.方法:用异戊烯焦磷酸法体外扩增人外周血γδT细胞.用不同浓度的阿司匹林诱导γδT细胞和消化道肿瘤SGC-7901、SW-1990、SW-480、SW-1116、LOVO细胞株, 用乳酸脱氢酶法测定γδT细胞的杀伤活性, 用流式细胞术(FCM)检测阿司匹林诱导前后的γδT细胞和SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞凋亡百分率.结果:γδT细胞培养10 d时从扩增前4.21%增加到70.35%.阿司匹林0.4~0.8 mmol/L诱导24 h后的γδT细胞对5种肿瘤细胞的杀伤活性最高, 浓度超过3.2 mmol/L时杀伤活性呈下降趋势;SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞经不同浓度的阿司匹林诱导24 h后除药物浓度在0.4 mmol/L时γδT细胞对SW-480、SW-1116和LOVO细胞株的杀伤活性略有增强外, 其他组与对照组比较无明显变化.3.2 mmol/L阿司匹林对γδT细胞诱导24 h的凋亡率(52.71%)明显高于SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株(分别为7.88%、8.89%、6.21%、4.47%和%3.67).结论:阿司匹林在临床常规使用的药物浓度时可增强γδT细胞杀伤肿瘤细胞作用, 超过这一浓度可明显抑制γδT细胞的增殖能力和杀伤活性及增加γδT细胞的凋亡率, 而对SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株作用不明显. 相似文献
6.
7.
8.
Objective To explore the effect of Fufangkushen on gastric cancer cell killing by human γδT cells. Methods Isopentenyl pyrophosphate method was used to amplify human peripheral blood γδT cells in vitro. Fufangkushen at various concentrations was used to induce γδT cells and gastric cancer cell lines SGC-7901 for 24 hours, MTr assays was used to detect inhibitory effect of Fufangkushen on these cell lines, LDH assays was used to measure the cytotoxic activity of γδT cells, and flow cytometry was used to detect apoptosis of γδT cells and SGC-7901 before and after the treatment. Results Ten days after cultivation, proliferation ra-tio of γδT cells increased from 4.21% to 70.35% and CD44 was up to 94.0%. Inhibitory rate of Fufangkush-en on SGC-7901 at various concentrations was significantly higher than that on γδT cells (22.3% vs-22.4%, P<0.05). The negative inhibitory ratio on γδT cells showed a dose-dependent manner with Fufangkushen's concentrations ranging from 1/5 to 1/400. γδT cells cytotoxic activity to SGC-7901 induced by Fufangkushen for 24 h was higher than control group, (83.6% vs 71.2%, P<0.05). Apoptotic rate was significantly lower in γδT cells than in SGC-7901 (4.64% vs49.23%, P<0.05). Conclusion Fufangkushen, within routine concentration ranges, can promote γδT cells' proliferation, inhibit tumor cell growth and enhance γδT cells' cytotoxic activity. This may be beneficial to tumor adoptive immunotherapy and provide evidence for the appli-cation of Fufangkushen in the treatment of tumors. 相似文献
9.
目的探讨手术分娩时机对母、婴血中CD4+CD25+CD127low调节性T细胞(Treg)的影响。方法正常初产妇60例,按分娩方式分为阴道分娩(VD)、择期手术分娩(PCS)、急诊手术分娩(ECS)三组,每组20例,用流式细胞仪检测分娩时母体外周血和新生儿脐血T细胞中Treg百分数。结果新生儿脐血中Treg三组间比较差异无统计学意义(P>0.05),但PCS组明显高于VD组(P<0.05);母体外周血中Treg,三组中ECS组>PCS组>VD组,ECS组显著性高于VD组(P<0.01);三组母体、新生儿血中Treg均无相关性(P>0.05)。结论 PCS时新生儿血中Treg增高,可能会对婴儿免疫系统发育有一定的影响;分娩过程异常的ECS母体血中Treg增高可能与产妇免疫失衡有关。 相似文献
10.