Dual Bioactivities of Essential Oil Extracted from the Leaves of Artemisia argyi as an Antimelanogenic versus Antioxidant Agent and Chemical Composition Analysis by GC/MS

The study was aimed at investigating the antimelanogenic and antioxidant properties of essential oil when extracted from the leaves of Artemisia argyi, then analyzing the chemical composition of the essential oil. The inhibitory effect of the essential oil on melanogenesis was evaluated by a mushroom tyrosinase activity assay and B16F10 melanoma cell model. The antioxidant capacity of the essential oil was assayed by spectrophotometric analysis, and the volatile chemical composition of the essential oil was analyzed with gas chromatography-mass spectrometry (GC/MS). The results revealed that the essential oil significantly inhibits mushroom tyrosinase activity (IC₅₀ = 19.16 mg/mL), down-regulates B16F10 intracellular tyrosinase activity and decreases the amount of melanin content in a dose-dependent pattern. Furthermore, the essential oil significantly scavenged 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzthiazoline- 6-sulphonic acid) ABTS radicals, showed an apparent reduction power as compared with metal-ion chelating activities. The chemicals constituents in the essential oil are ether (23.66%), alcohols (16.72%), sesquiterpenes (15.21%), esters (11.78%), monoterpenes (11.63%), ketones (6.09%), aromatic compounds (5.01%), and account for a 90.10% analysis of its chemical composition. It is predicted that eucalyptol and the other constituents, except for alcohols, in the essential oil may contribute to its antioxidant activities. The results indicated that essential oil extracted from A. argyi leaves decreased melanin production in B16F10 cells and showed potent antioxidant activity. The essential oil can thereby be applied as an inhibitor of melanogenesis and could also act as a natural antioxidant in skin care products.     

Analysis and Comparison of Location Strategies for Reducing Registration Cost in PCS Networks

In mobile environments, a personal communication service (PCS) network must keep track of the location of each mobile user in order to correctly deliver calls. A basic scheme used in the standard IS-41 and GSM protocols is to always update the location of a mobile user whenever the mobile user moves to a new location. The problem with this approach is that the cost of location update operations is very high especially when the mobile user moves frequently. In recent years, various location management strategies for reducing the location update cost have been proposed. However, the performance issue of these proposed algorithms remains to be investigated. In this paper, we develop two Markov chains to analyze and compare the performance of two promising location update strategies, i.e., the two location algorithm (TLA) and the forwarding and resetting algorithm (FRA). By utilizing the Markov chain, we are able to quickly answer what-if questions regarding the performance of PCS networks under various workload conditions and also identify conditions under which one strategy can perform better than the others. Using the cost due to location update and search operations between two successive calls to a mobile user as a performance measure, we show that when the mobile user exhibits a high degree of locality and the mobile user's call-to-mobility ratio (CMR) is low, TLA can significantly outperform both FRA and IS-41. On the other hand, when CMR is high, FRA is the winner. Furthermore, unlike TLA which may perform worse than IS-41 at high CMR values, FRA at identified optimal conditions can always perform at least as good as IS-41, suggesting that FRA over TLA should be used as a generic scheme in reducing the location cost in the PCS network to cover all possible per-user CMR values.     

Agent-Based Forwarding Strategies for Reducing Location Management Cost in Mobile Networks

An important issue in location management for dealing with user mobility in wireless networks is to reduce the cost associated with location updates and searches. The former operation occurs when a mobile user moves to a new location registration area and the network is being informed of the mobile user's current location; the latter operation occurs when there is a call for the mobile user and the network must deliver the call to the mobile user. In this paper, we propose and analyze a class of new agent-based forwarding schemes with the objective to reduce the location management cost in mobile wireless networks. We develop analytical models to compare the performance of the proposed schemes with existing location management schemes to demonstrate their feasibility and also to reveal conditions under which our proposed schemes are superior to existing ones. Our proposed schemes are particularly suitable for mobile networks with switches which can cover a large number of location registration areas.     

Antioxidative Characteristics of Anisomeles indica Extract and Inhibitory Effect of Ovatodiolide on Melanogenesis

The purpose of the study was to investigate the antioxidant characteristics of Anisomeles indica methanol extract and the inhibitory effect of ovatodiolide on melanogenesis. In the study, the antioxidant capacities of A. indica methanol extract such as DPPH assay, ABTS radical scavenging assay, reducing capacity and metal ion chelating capacity as well as total phenolic content of the extract were investigated. In addition, the inhibitory effects of ovatodiolide on mushroom tyrosinase, B16F10 intracellular tyrosinase and melanin content were determined spectrophotometrically. Our results revealed that the antioxidant capacities of A. indica methanol extract increased in a dose-dependent pattern. The purified ovatodiolide inhibited mushroom tyrosinase activity (IC(50) = 0.253 mM), the compound also effectively suppressed intracellular tyrosinase activity (IC(50) = 0.469 mM) and decreased the amount of melanin (IC(50) = 0.435 mM) in a dose-dependent manner in B16F10 cells. Our results concluded that A. indica methanol extract displays antioxidant capacities and ovatodiolide purified from the extract inhibited melanogenesis in B16F10 cells. Hence, A. indica methanol extract and ovatodiolide could be applied as a type of dermatological whitening agent in skin care products.     

Identification of two licorice species, Glycyrrhiza uralensis and Glycyrrhiza glabra, based on separation and identification of their bioactive components

The purpose of this study was to identify two licorice species, Glycyrrhiza uralensis and Glycyrrhiza glabra. A simple method including HPLC and LC/MS was developed to simultaneously separate, identify, and quantify 15 bioactive components of Glycyrrhiza species. G. uralensis was found to contain quercetin but G. glabra did not. G. glabra provided by Brion Research Institute of Taiwan contained uralsaponin B, but G. glabra brought from herbal shops did not. The DPPH radical scavenging activity of G. uralensis and G. glabra achieved approximately 72–75% if 10 mg/mL or more licorice extract was used. G. uralensis had slightly better radical scavenging activity than G. glabra. G. uralensis also showed higher reducing ability than G. glabra.     

Investigation of the Anti-Melanogenic and Antioxidant Characteristics of Eucalyptus camaldulensis Flower Essential Oil and Determination of Its Chemical Composition

The effects of essential oil from Eucalyptus camaldulensis flowers oil on melanogenesis and the oil’s antioxidant characteristics were investigated. Assays of mushroom and cellular tyrosinase activities and melanin content of mouse melanoma cells were performed spectrophotometrically, and the expression of melanogenesis-related proteins was determined by Western blotting. The possible signaling pathways involved in essential oil-mediated depigmentation were also investigated using specific protein kinase inhibitors. The results revealed that E. camaldulensis flower essential oil effectively suppresses intracellular tyrosinase activity and decreases melanin amount in B16F10 mouse melanoma cells. The essential oil also exhibits antioxidant properties and effectively decreases intracellular reactive oxygen species (ROS) levels. The volatile chemical composition of the essential oil was analyzed with gas chromatography–mass spectrometry (GC/MS). The chemical constituents in the essential oil are predominately oxygenated monoterpenes (34.9%), followed by oxygenated sesquiterpenes (31.8%), monoterpene hydrocarbons (29.0%) and sesquiterpene hydrocarbons (4.3%). Our results indicated that E. camaldulensis flower essential oil inhibits melanogenesis through its antioxidant properties and by down-regulating both mitogen-activated protein kinases (MAPK) and protein kinase A (PKA) signaling pathways. The present study indicates that the essential oil has the potential to be developed into a skin care product.