Evaluation of Digitally Encoded Layer‐by‐layer Coated Microparticles as Cell Carriers

To obtain more biologically relevant data there is a growing interest in the use of living cells for assaying the biological activity of unknown chemical compounds. Density ‘multiplex’ cell‐based assays, where different cell types are mixed in one well and simultaneously investigated upon exposure to a certain compound are beginning to emerge. To be able to identify the cells they should be attached to microscopic carriers that are encoded. This paper investigates how digitally encoded microparticles can be loaded with cells while keeping the digital code in the microcarriers readable. It turns out that coating the surface of the encoded microcarriers with polyelectrolytes using the layer‐by‐layer (LbL) approach provides the microcarriers with a ‘highly functional’ surface. The polyelectrolyte layer allows the growth of the cells, allows the orientation of the cell loaded microcarriers in a magnetic field, and does not hamper the reading of the code. It has further been shown that the cells growing on the polyelectrolyte layer can become transduced by adenoviral particles hosted by the polyelectrolyte layer. It is concluded that the digitally encoded microparticles are promising materials for use in biomedical and pharmaceutical in‐vitro research where cells are used as tools.     

Carbon mineralization from composts and food industry wastes added to soil

We have studied the short term C mineralization of six wastes from important food industries, one sludge from a biogas plant and three composts. All the wastes were characterized chemically and fractionated according to the Van Soest method. The fresh wastes were incubated under controlled environment conditions to determine the C mineralization rate. Based on first order mineralization kinetics, we calculated the hypothetical amount of stable C in the wastes as the amount of C that would not be mineralized within one year under field conditions. The percentage of stable organic C in the organic matter was in general much larger in the composts than in the other wastes, but when expressed on dry matter, the non-composted wastes had comparable or larger amounts of stable organic C than the composts and have a considerable potential for supplying organic matter to soils, and hence for C sequestration. The amount of stable organic C could best be predicted by the total N content of the wastes (R _a ² = 0.855), whereas the results of the fractionation had very little predictive power, probably due to problems related to the high ash content of some of the wastes. An index that combined stable organic C and N and P content in the wastes was calculated to assess possible limitations for applying these wastes in agriculture. Under current nutrient legislation in Western Europe, a number of these wastes will only be usable in small amounts, but these and other food industry wastes could still prove to be valuable soil amendments in nutrient poor situations, for increasing soil organic C content and supplying nutrients.     

New driving scheme for intelligent power‐efficient high‐voltage display drivers

Abstract— A new bistable‐display driver is presented. The innovation in the developed driver is the addition of a new logical block that calculates the most energy‐efficient driving waveforms. In this paper, the algorithms being applied to the row and column waveforms in order to reduce the power consumption are discussed. Some theoretical as well as experimental results are shown, proving a reduction in the power consumption by about 50%. The proposed algorithms are especially important for battery‐powered applications.     

Exhibiting Architecture à la Manière de

Contemporary architecture exhibitions constitute an adventurous domain involving architects, artists, curators, and scholars in various roles. Not only do architecture exhibitions mediate designs and research, they advance particular auteur approaches to the very practice of exhibiting. This article presents a hands-on study of selected exhibition authors' means and strategies in making architecture exhibitions. The basis for comparison in this research-by-design project is a brief to display student work in the context of an architecture department.     

Effect of muscle,ageing time and modified atmosphere packaging conditions on the colour,oxidative and microbiological stability of packed beef

The objective of this study was to evaluate the influence of two different vacuum ageing times (7 and 14 days) and the impact of the modified atmosphere packaging (MAP) configuration (gas/product ratios: 0.5 and 1 and gas composition: 70% O₂ + 30% CO₂ and 40% O₂ + 30% CO₂ + 30% N₂) on the quality of fresh beef during subsequent storage at 4 °C. For this purpose, three separate experiments were performed. For each experiment, two different muscles (Longissimus dorsi and Biceps femoris) were sampled from four double‐muscled Belgian Blue beef carcasses. Next to colour, also the evolution in microbial load, pH, O₂ and CO₂ in the headspace and lipid oxidation at the meat surface were evaluated. A vacuum ageing for 14 days compared with 7 days resulted in a higher initial microbial load on the day of MAP packaging, which resulted finally in a significantly shorter shelf life. This ageing effect was less pronounced on the colour stability and lipid oxidation of the meat samples. No significant influence of the packaging configuration on any of the analysed parameters (colour, microbial load, pH and lipid oxidation at the meat surface) was observed.     

Identification of the impedance model of an implanted cochlear prosthesis from intracochlear potential measurements

Those suffering from a severe to profound sensorineural hearing loss can obtain substantial benefit from a cochlear implant prosthesis. An electrode array implanted in the inner ear stimulates auditory nerve fibers by direct injection of electrical current. A major limitation of today's technology is the imprecise control of intracochlear current flow, particularly the relatively wide spread of neural excitation. A better understanding of the intracochlear electrical fields is, therefore, required. This paper analyzes the structure of intracochlear potential measurements in relation to both the subject's anatomy and to the properties of the electrode array. An electrically equivalent network is proposed, composed of small lumped circuits for the interface impedance and for the cochlear tissues. The numerical methods required to estimate the model parameters from high-quality electrical potential recordings are developed. Finally, some models are presented for subjects wearing a Clarion CII device with a HiFocus electrode and discussed in terms of model reliability.     

Encoding microcarriers by spatial selective photobleaching

Bead-based assays on very large numbers of molecules in gene expression studies, drug screening and clinical diagnostics, require the encoding of each of the microspheres according to the particular ligand bound to its surface. This allows mixing the uniquely encoded microspheres and subjecting them to an assay simultaneously. When a particular microsphere gives a positive reaction, the substance on its surface can be identified by reading the code. Previously reported techniques for colour encoding polymer microspheres only allow for a limited number of unique codes. Graphical encoding methods use metallic particles, which are rather uncommon in screening applications. Here, we demonstrate a new approach to encode polymer microspheres that are commonly used in screening applications, such as polystyrene microspheres, with a method that provides a virtually unlimited number of unique codes. Patterns can be written in fluorescently dyed microspheres by 'spatial selective photobleaching' and can be identified by confocal microscopy. Such encoded microparticles can find broad application in the collection and analysis of genetic information, high-throughput screening, medical diagnostics and combinatorial chemistry, and can also be used for labelling of consumer goods or as security labels to prevent counterfeiting.     

提高FPGA-PWM计数器性能的位交换

当你需要一些模拟输出,并且系统中有一片FPGA时,你可能选择采用一个PWM模块和一个简单的低通滤波器,如图1所示.FPGA的输出通常是一种固定频率、可变占空比的波形,由一个计数器和一个数字比较器生成(表1).     

Evaluation of pre/post-fire differenced spectral indices for assessing burn severity in a Mediterranean environment with Landsat Thematic Mapper

In this study several pre/post-fire differenced spectral indices for assessing burn severity in a Mediterranean environment are evaluated. GeoCBI (Geo Composite Burn Index) field data of burn severity were correlated with remotely sensed measures, based on the NBR (Normalized Burn Ratio), the NDMI (Normalized Difference Moisture Index) and the NDVI (Normalized Difference Vegetation Index). In addition, the strength of the correlation was evaluated for specific fuel types and the influence of the regression model type is pointed out. The NBR was the best remotely sensed index for assessing burn severity, followed by the NDMI and the NDVI. For this case study of the 2007 Peloponnese fires, results show that the GeoCBI–dNBR (differenced NBR) approach yields a moderate–high R ²?=?0.65. Absolute indices outperformed their relative equivalents, which accounted for pre-fire vegetation state. The GeoCBI–dNBR relationship was stronger for forested ecotypes than for shrub lands. The relationship between the field data and the dNBR and dNDMI (differenced NDMI) was nonlinear, while the GeoCBI–dNDVI (differenced NDVI) relationship appeared linear.