The Age-Sensitive Efficacy of Calorie Restriction on Mitochondrial Biogenesis and mtDNA Damage in Rat Liver

Calorie restriction (CR) is the most efficacious treatment to delay the onset of age-related changes such as mitochondrial dysfunction. However, the sensitivity of mitochondrial markers to CR and the age-related boundaries of CR efficacy are not fully elucidated. We used liver samples from ad libitum-fed (AL) rats divided in: 18-month-old (AL-18), 28-month-old (AL-28), and 32-month-old (AL-32) groups, and from CR-treated (CR) 28-month-old (CR-28) and 32-month-old (CR-32) counterparts to assay the effect of CR on several mitochondrial markers. The age-related decreases in citrate synthase activity, in TFAM, MFN2, and DRP1 protein amounts and in the mtDNA content in the AL-28 group were prevented in CR-28 counterparts. Accordingly, CR reduced oxidative mtDNA damage assessed through the incidence of oxidized purines at specific mtDNA regions in CR-28 animals. These findings support the anti-aging effect of CR up to 28 months. Conversely, the protein amounts of LonP1, Cyt c, OGG1, and APE1 and the 4.8 Kb mtDNA deletion content were not affected in CR-28 rats. The absence of significant differences between the AL-32 values and the CR-32 counterparts suggests an age-related boundary of CR efficacy at this age. However, this only partially curtails the CR benefits in counteracting the generalized aging decline and the related mitochondrial involvement.     

Tetanus toxin-sensitive VAMP-related proteins are present in murine macrophages

The light chain of tetanus neurotoxin (TeTx) is a zinc endopeptidase specific for VAMP/synaptobrevin (VAMP), a 120-amino-acid integral protein previously described in the small synaptic vesicles of neuronal cells. TeTx has been shown to be active also on nonneuronal cells. By SDS-PAGE and quantitative immunoblotting on proteins derived from murine macrophages (Mphi) exposed to TeTx, we have shown that: (1) VAMP-related proteins are also present in Mphi and (2) such proteins are sensitive to TeTx proteolytic cleavage. The demonstration that TeTx acts on VAMP-related proteins also in Mphi offers a new and useful tool for molecular studies on Mphi exocytosis.     

The Ketogenic Diet Reduces the Harmful Effects of Stress on Gut Mitochondrial Biogenesis in a Rat Model of Irritable Bowel Syndrome

Functional alterations in irritable bowel syndrome have been associated with defects in bioenergetics and the mitochondrial network. Effects of high fat, adequate-protein, low carbohydrate ketogenic diet (KD) involve oxidative stress, inflammation, mitochondrial function, and biogenesis. The aim was to evaluate the KD efficacy in reducing the effects of stress on gut mitochondria. Newborn Wistar rats were exposed to maternal deprivation to induce IBS in adulthood. Intestinal inflammation (COX-2 and TRL-4); cellular redox status (SOD 1, SOD 2, PrxIII, mtDNA oxidatively modified purines); mitochondrial biogenesis (PPAR-γ, PGC-1α, COX-4, mtDNA content); and autophagy (Beclin-1, LC3 II) were evaluated in the colon of exposed rats fed with KD (IBD-KD) or standard diet (IBS-Std), and in unexposed controls (Ctrl). IBS-Std rats showed dysfunctional mitochondrial biogenesis (PPAR-γ, PGC-1α, COX-4, and mtDNA contents lower than in Ctrl) associated with inflammation and increased oxidative stress (higher levels of COX-2 and TLR-4, SOD 1, SOD 2, PrxIII, and oxidatively modified purines than in Ctrl). Loss of autophagy efficacy appeared from reduced levels of Beclin-1 and LC3 II. Feeding of animals with KD elicited compensatory mechanisms able to reduce inflammation, oxidative stress, restore mitochondrial function, and baseline autophagy, possibly via the upregulation of the PPAR-γ/PGC-1α axis.     

A complexity-bounded motion estimation algorithm

The full search motion estimation algorithm for video coding is a procedure of high computational cost. For this reason, in real-time low-power applications, low-cost motion estimation algorithms are viable solutions. A novel reduced complexity motion estimation algorithm is presented. It conjugates the reduction of computational load with good encoding efficiency. It exploits the past history of the motion field to predict the current motion field. A successive refinement phase gives the final motion field. This approach leads to a sensible reduction in the number of motion vector that have to be tested. The complexity is lower than any other algorithm algorithms known to the authors, in the literature, it is constant as there is no recursivity in the algorithm and independent of any search window area size. Experimental evaluations have shown the robustness of the algorithm when applied on a wide set of video sequences-a good performance compared to other reduced complexity algorithms and negligible loss of efficiency versus the full search algorithm.     

MPEG-2 sources: exploiting source scalability for an efficient bandwidth allocation

The paper investigates efficient bandwidth allocation schemes for the transmission of MPEG-2 video traffic on high-speed networks. To this end we performed an extensive analysis of the traffic generated by an MPEG-2 encoder. Specifically, we encoded “The Sheltering Sky” movie according to the MPEG-2 standard. By the analysis of the generated traffic it results that a constant-quality transmission can be performed with a poor bandwidth utilization. In the paper we identified that the low bandwidth utilization is caused by rare high-rate periods in the codec bitstream. Hence, we identified the source scalability as a promising approach to achieve a “quasi-constant” quality transmission and an efficient bandwidth utilization. The effectiveness of this approach is evaluated in the paper via simulation. Specifically, by defining a Markovian model for an MPEG-2 scalable source we performed a set of simulation experiments which indicate that the source scalability approach significantly increases the utilization, while maintaining the quality of the video signal at the highest value for most of the time, e.g., a 50% of the network utilization with the highest quality for the 99.7% of the time.     

Variational techniques for assessing the technological signature of flat surfaces

The quality assessment of manufacturing operations performed to obtain given flat surfaces is always a problem of comparing the substitute model (approximating the features of the true manufactured part) to the nominal specifications, at any stage of the manufacturing cycle. A novel methodology, based on applications of classical tools of Calculus of Variations, is here presented with the aim of assessing the output quality of manufactured flat surfaces based on the information available on transformation imposed by technological processes. By assuming that any manufacturing process operates under equilibrium states, the proposed variational methodology allows to account for the traces left by different stages of manufacturing processes. A simple two-dimensional case is here discussed, to give the flavor of the methodology and its future potential developments.     

MPEG-2 Coded Video Traces Transmitted Over a Satellite Link: Scalable and Non-Scalable Solutions in Rain Fading Conditions

The literature is poor in the analyses of the effects produced by corrupted bits in compressed video bitstreams. This paper presents the results of a transmission experiment of MPEG-2 coded video data over a satellite link affected by noise, in order to investigate under which conditions this type of transmission is economically feasible. The signal-to-noise ratio scalability feature of the MPEG-2 encoder was used to produce different bitstreams of the same movie sequence. The scope of the study was to verify which are the best combinations of video and channel codings in the presence of attenuation on the satellite link, in order to optimize the bandwidth utilisation for a requested image quality. The results obtained give indications about the data channel codings to be used to counter the rain fade on the transmission link, which is a non negligible problem especially when satellite transmissions are in the Ka band. Moreover, the results highlight the flexibility of the scalable video coding in the examined scenario.