全文获取类型
收费全文 | 937篇 |
免费 | 80篇 |
国内免费 | 2篇 |
学科分类
工业技术 | 1019篇 |
出版年
2024年 | 1篇 |
2023年 | 11篇 |
2022年 | 17篇 |
2021年 | 78篇 |
2020年 | 27篇 |
2019年 | 45篇 |
2018年 | 39篇 |
2017年 | 38篇 |
2016年 | 44篇 |
2015年 | 36篇 |
2014年 | 50篇 |
2013年 | 71篇 |
2012年 | 80篇 |
2011年 | 83篇 |
2010年 | 54篇 |
2009年 | 44篇 |
2008年 | 57篇 |
2007年 | 52篇 |
2006年 | 39篇 |
2005年 | 30篇 |
2004年 | 24篇 |
2003年 | 17篇 |
2002年 | 13篇 |
2001年 | 7篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 9篇 |
1997年 | 9篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 8篇 |
1993年 | 9篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1987年 | 2篇 |
1984年 | 1篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有1019条查询结果,搜索用时 46 毫秒
1.
Sinenhlanhla N. Mthembu Dr. Anamika Sharma Prof. Fernando Albericio Prof. Beatriz G. de la Torre 《Chembiochem : a European journal of chemical biology》2020,21(14):1947-1954
Cysteine is present in a large number of natural and synthetic (bio)molecules. Although the thiol side chain of Cys can be in a free form, in most cases it forms a disulfide bond either with a second Cys (bridge) or with another thiol, as in the case of protecting groups. Efficient reduction of these disulfide bridges is a requirement for many applications of Cys-containing molecules in the fields of chemistry and biochemistry. Here we review reducing methods for disulfide bonds, taking into consideration the solubility of the substrates when selecting the appropriate reducing reagent. 相似文献
2.
Magalí Rey-Campos Beatriz Novoa Alberto Pallavicini Marco Gerdol Antonio Figueras 《International journal of molecular sciences》2021,22(6)
Mytimycins are cysteine-rich antimicrobial peptides that show antifungal properties. These peptides are part of the immune network that constitutes the defense system of the Mediterranean mussel (Mytilus galloprovincialis). The immune system of mussels has been increasingly studied in the last decade due to its great efficiency, since these molluscs, particularly resistant to adverse conditions and pathogens, are present all over the world, being considered as an invasive species. The recent sequencing of the mussel genome has greatly simplified the genetic study of some of its immune genes. In the present work, we describe a total of 106 different mytimycin variants in 16 individual mussel genomes. The 13 highly supported mytimycin clusters (A–M) identified with phylogenetic inference were found to be subject to the presence/absence variation, a widespread phenomenon in mussels. We also identified a block of conserved residues evolving under purifying selection, which may indicate the “functional core” of the mature peptide, and a conserved set of 10 invariable plus 6 accessory cysteines which constitute a plastic disulfide array. Finally, we extended the taxonomic range of distribution of mytimycins among Mytilida, identifying novel sequences in M. coruscus, M. californianus, P. viridis, L. fortunei, M. philippinarum, M. modiolus, and P. purpuratus. 相似文献
3.
4.
5.
Natalia Brossard Beatriz Gonzalez-Muñoz Carolina Pavez Arianna Ricci Xinmiao Wang Fernando Osorio Edmundo Bordeu Giuseppina Paola Parpinello Jianshe Chen 《International Journal of Food Science & Technology》2021,56(10):5382-5394
Astringency is a sensory attribute, related to the quality and mouthfeel of red wines. However, the origin of astringency sub-qualities, such as the typical drying astringency found in immature grapes, is still unknown. Astringency of red wines with similar tannin content but different astringency sub-qualities, from different harvest dates, is studied. Astringency was characterised in terms of friction coefficient, polyphenol content, sensory analysis and tannin/salivary–proteins aggregates characterisation. A different evolution during ripening was found for both Cabernet Sauvignon and Carménère, and tannin–protein aggregates showed differences in size, shape and surface. The velvety sub-quality appears to be related to aggregates with low precipitation, and with specific surface characteristics as roundness and Feret diameter. Results from this work propose an effect of aggregates on sensory perception and opens the possibility to explore their effect on oral lubrication. 相似文献
6.
Liyun Yang Ke Lu Mikael Forsman Kaj Lindecrantz Fernando Seoane Örjan Ekblom 《Ergonomics》2019,62(5):694-705
Work metabolism (WM) can be accurately estimated by oxygen consumption (VO2), which is commonly assessed by heart rate (HR) in field studies. However, the VO2–HR relationship is influenced by individual capacity and activity characteristics. The purpose of this study was to evaluate three models for estimating WM compared with indirect calorimetry, during simulated work activities. The techniques were: the HR-Flex model; HR branched model, combining HR with hip-worn accelerometers (ACC); and HR?+?arm-leg ACC model, combining HR with wrist- and thigh-worn ACC. Twelve participants performed five simulated work activities and three submaximal tests. The HR?+?arm-leg ACC model had the overall best performance with limits of agreement (LoA) of ?3.94 and 2.00?mL/min/kg, while the HR-Flex model had ?5.01 and 5.36?mL/min/kg and the branched model, ?6.71 and 1.52?mL/min/kg. In conclusion, the HR?+?arm-leg ACC model should, when feasible, be preferred in wearable systems for WM estimation.
Practitioner Summary: Work with high energy demand can impair employees’ health and life quality. Three models were evaluated for estimating work metabolism during simulated tasks. The model combining heart rate, wrist- and thigh-worn accelerometers showed the best accuracy. This is, when feasible, suggested for wearable systems to assess work metabolism. 相似文献
7.
Dr. Héctor Zamora-Carreras Dr. Beatriz Maestro Dr. Jesús M. Sanz Dr. M. Angeles Jiménez 《Chembiochem : a European journal of chemical biology》2020,21(4):432-441
A common interpretation of Anfinsen's hypothesis states that one amino acid sequence should fold into a single, native, ordered state, or a highly similar set thereof, coinciding with the global minimum in the folding-energy landscape, which, in turn, is responsible for the function of the protein. However, this classical view is challenged by many proteins and peptide sequences, which can adopt exchangeable, significantly dissimilar conformations that even fulfill different biological roles. The similarities and differences of concepts related to these proteins, mainly chameleon sequences, metamorphic proteins, and switch peptides, which are all denoted herein “turncoat” polypeptides, are reviewed. As well as adding a twist to the conventional view of protein folding, the lack of structural definition adds clear versatility to the activity of proteins and can be used as a tool for protein design and further application in biotechnology and biomedicine. 相似文献
8.
9.
10.
Beatriz García-Fontana Sonia Morales-Santana Victoria Longobardo Rebeca Reyes-García Pedro Rozas-Moreno José Antonio García-Salcedo Manuel Mu?oz-Torres 《International journal of molecular sciences》2015,16(5):9469-9483
Type 2 diabetes mellitus patients are at significant risk of cardiovascular disease, however, the pathophysiology of these complications is complex and incompletely known in this population. The aim of this study was to compare the serum proteome of patients with type 2 diabetes mellitus presenting or not presenting cardiovascular disease with non-diabetic subjects to find essential proteins related to these cardiovascular complications. This cross-sectional study compares the serum proteome by a combination of protein depletion with 2D-DIGE (2-dimension Difference Gel Electrophoresis) methodology. The proteins differentially expressed were identified by MALDI TOF/TOF (Matrix-assisted laser desorption/ionization and Time-Of-Flight ion detector) or LC-MS/MS (Liquid Chromatography coupled to Mass-Mass Spectrometry). Type 2 diabetes mellitus patients with cardiovascular disease showed higher expression of plasma retinol binding protein and glutathione peroxidase-3 compared to those without cardiovascular disease and non-diabetic controls. These results show that proteins related to the inflammatory and redox state appear to play an important role in the pathogenesis of the cardiovascular disease in the type 2 diabetes mellitus patients. 相似文献