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1.
Specificity of Lipoprotein Chaperones for the Characteristic Lipidated Structural Motifs of their Cognate Lipoproteins 下载免费PDF全文
Dr. Tom Mejuch Dr. Hilde van Hattum Dr. Gemma Triola Dr. Mamta Jaiswal Prof. Dr. Herbert Waldmann 《Chembiochem : a European journal of chemical biology》2015,16(17):2460-2465
Lipoprotein‐binding chaperones mediate intracellular transport of lipidated proteins and determine their proper localisation and functioning. Understanding of the exact structural parameters that determine recognition and transport by different chaperones is of major interest. We have synthesised several lipid‐modified peptides, representative of different lipoprotein classes, and have investigated their binding to the relevant chaperones PDEδ, UNC119a, UNC119b, and galectins‐1 and ‐3. Our results demonstrate that PDEδ recognises S‐isoprenylated C‐terminal peptidic structures but not N‐myristoylated peptides. In contrast, UNC119 proteins bind only mono‐N‐myristoylated, but do not recognise doubly lipidated and S‐isoprenylated peptides at the C terminus. For galectins‐1 and ‐3, neither binding to N‐acylated, nor to C‐terminally prenylated peptides could be determined. These results shed light on the specificity of the chaperone‐mediated cellular lipoprotein transport systems. 相似文献
2.
Carbon dioxide emission and its regulation at land–water interface downstream of a point source at Ganga River (India) 下载免费PDF全文
Deepa Jaiswal Ekabal Siddiqui Kavita Verma Jitendra Pandey 《Water and Environment Journal》2018,32(3):351-357
The streams and rivers are considered hotspots of CO2 exchange; and representative direct CO2 emission measurements are essential for a correct regional estimate. We measured CO2 emission flux at 15 sites at land–water interface downstream of a point source during low flow for three consecutive months for the year 2017. The general range of CO2 efflux observed here was close to the results of regional studies, although values near the point source were disproportionately high (>350 mg/m2/h). CO2 emission flux showed strong dependence on total organic carbon (TOC; R2 = 0.96; P < 0.001), (R2 = 0.88; P < 0.001), soluble reactive‐P (SRP; R2 = 0.91; P < 0.001) and microbial activity measured in terms of fluorescein diacetate activity (FDAase; R2 = 0.92; P < 0.001) and substrate induced respiration (SIR; R2 = 0.96; P < 0.001). Because point source‐associated interfaces provide heterogeneous habitats, our study suggests the need for large scale monitoring of CO2 emission at land–water interface of major rivers for more correctly presenting the regional scale CO2 budget. 相似文献
3.
Ceria (La3+, Sr2+)/Carbonates Nanocomposite Electrolytes with High Electrical Conductivity for Low‐Temperature SOFCs 下载免费PDF全文
Nandini Jaiswal Devendra Kumar Om Parkash Shail Upadhyay 《International Journal of Applied Ceramic Technology》2015,12(5):1080-1087
A series of ceria‐based nanocomposites consisting of lanthanum and strontium codoped ceria with composition Ce0.89La0.07Sr0.04O1.925 (CL7S4) and eutectic mixture of carbonates Li2CO3‐Na2CO3 (LNCO) have been prepared by mixing nanosize powders of CL7S4 and LNCO. Samples have been characterized using differential thermal analysis, X‐ray diffraction, scanning electron microscopy combined with energy‐dispersive spectroscopy, thermal expansion, and impedance spectroscopy. A sharp increase in ionic conductivity is observed in all the composite specimens corresponding to superionic transition. Sample containing 35 wt% of carbonate shows the maximum conductivity (2.56 × 10?1 S/cm at 500°C) with activation energy of conduction, Ea 0.23 eV. 相似文献
4.
Ganesh Swain Ravi Kumar Sonwani Balendu Shekhar Giri Ram Sharan Singh Ravi Prakash Jaiswal Birendra Nath Rai 《Water and Environment Journal》2021,35(1):285-294
The impact of external mass transport on the biodegradation rate of phenol in a packed bed bioreactor (PBBR) was studied. A potential bacterial species, Bacillus flexus GS1 IIT (BHU), was isolated from the petroleum‐contaminated soil. Low‐density polyethylene (LDPE) immobilized with the B. flexus GS1 IIT (BHU) was used as packing material in the PBBR. The PBBR was operated by varying the inlet feed flow rate from 4 to 10 mL/min, and the corresponding degradation rate coefficients were found to be in the range of 0.119–0.157 L/g h. In addition, the highest removal rate of phenol was obtained to be 1.305 mg/g h at an inlet feed rate of 10 mL/min. The external mass transfer was studied using the model . A new empirical correlation for the biodegradation of phenol in the PBBR was developed after the evaluation at various values of K and n. 相似文献
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Amit K. Jaiswal Hemlata Chhabra Sachin S. Kadam Kishore Londhe Vivek P. Soni Jayesh R. Bellare 《Materials science & engineering. C, Materials for biological applications》2013,33(5):2926-2936
The aim of this study was to compare physico-chemical and biological properties of hydroxyapatite (HA) and hardystonite (HS) based composite scaffolds. Hardystonite (Ca2ZnSi2O7) powders were synthesized by a sol–gel method while polycaprolactone–hardystonite (PCL–HS) and polycaprolactone–hydroxyapatite (PCL–HA) were fabricated in nanofibrous form by electrospinning. The physico-chemical and biological properties such as tensile strength, cell proliferation, cell infiltration and alkaline phosphatase activity were determined on both kinds of scaffolds. We found that PCL–HS scaffolds had better mechanical strength compared to PCL–HA scaffolds. Addition of HA and HS particles to PCL did not show any inhibitory effect on blood biocompatibility of scaffolds when assessed by hemolysis assay. The in vitro cellular behavior was evaluated by growing murine adipose-tissue-derived stem cells (mE-ASCs) over the scaffolds. Enhanced cell proliferation and improved cellular infiltrations on PCL–HS scaffolds were observed when compared to HA containing scaffolds. PCL–HS scaffolds exhibited a significant increase in alkaline phosphatase (ALP) activity and better mineralization of the matrix in comparison to PCL–HA scaffolds. These results clearly demonstrate the stimulatory role of Zn and Si present in HS based composite scaffolds, suggesting their potential application for bone tissue engineering. 相似文献
7.
A. Ananthanarayanan S. A. Khot V. Jaiswal A. Joseph P. P. Songire D. Banerjee 《The International journal of environmental studies》2017,74(2):192-201
Ag loaded mordenite can be used as a trap for radio-iodine arising from nuclear reprocessing operations. Typically, iodine is trapped in the Ag loaded mordenite by the formation of AgI in the pores of the mordenite, through a solid-vapour reaction. In the presence of NOx and water vapour, AgI is most likely formed by liquid-vapour reaction between AgNO3 and I2. This reaction results in the formation of large aggregates of AgI crystals on the surface of the mordenite, also leading to a yellow colouration of the mordenite, which is not observed when NOx and water vapour is absent. 相似文献
8.
Rapid detection and quantification of soya bean oil and common sugar in bovine milk using attenuated total reflectance–fourier transform infrared spectroscopy 下载免费PDF全文
Pranita Jaiswal Shyam Narayan Jha Jaspreet Kaur Ramya Hg 《International Journal of Dairy Technology》2018,71(2):292-300
Attenuated total reflectance–Fourier transform infrared spectroscopy, along with chemometrics, were used to detect and quantify soya bean oil (SO) and sugar (CS) adulteration in milk. Bovine milk was artificially adulterated with SO (0.2–2.0%; v/v) and CS (1–10%; w/v) separately. Spectra revealed significant differences in specific wavenumber regions (SO: 1450–1250 cm?1; CS: 1200–900 cm?1). Soya bean oil adulteration was best predicted in wavenumber range of 1262–1164 cm?1, using partial least square regression (coefficient of determination (R2: 0.90 and 0.88 for calibration and validation, respectively). Common sugar adulteration was best predicted in wavenumber range of 1010–910 cm?1 (R2: 0.99 for calibration and validation) using partial least square. 相似文献
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Drawbacks of poly(methyl methacrylate) (PMMA)‐based bone cement as a grouting agent for in vivo fixation of orthopedic and dental implants such as considerable low mechanical strength have been improved using nanotechnology. Bone cement‐layered silicate nanocomposites have been prepared without any heat treatment in the presence of polar (dimethyl formamide, DMF) and nonpolar (benzene) solvents. Solvents have been removed completely from the bone cement after its preparation. Nanostructure is very much dependent on the solvent used for nanocomposite preparation, and benzene‐based nanocomposites are highly intercalated, whereas DMF‐based nanocomposites do not exhibit intercalation. Thermal stability of bone cement has improved in the presence of nanoclays. The relative enhanced interaction in case of benzene‐based nanocomposites has been shown through FTIR and UV–vis studies. The significant improvement in modulus and toughness of bone cement has been demonstrated in the presence of minimum amount of nanoclay for benzene‐based nanocomposites, whereas no change in modulus and reduced toughness have been observed for DMF‐based nanocomposites. The decrease of contact angle has been witnessed with increasing nanoclay concentration indicating better hydrophilic materials suitable for biomedical applications for greater cell growth. The reason for varying property enhancement in different solvents has been discussed considering the polarity effect and interactions. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011 相似文献