Milk from cows fed a diet with a high forage:concentrate ratio improves inflammatory state,oxidative stress,and mitochondrial function in rats

Excessive energy intake may evoke complex biochemical processes characterized by inflammation, oxidative stress, and impairment of mitochondrial function that represent the main factors underlying noncommunicable diseases. Because cow milk is widely used for human nutrition and in food industry processing, the nutritional quality of milk is of special interest with respect to human health. In our study, we analyzed milk produced by dairy cows fed a diet characterized by a high forage:concentrate ratio (high forage milk, HFM). In view of the low n-6:n-3 ratio and high content of conjugated linoleic acid of HFM, we studied the effects of this milk on lipid metabolism, inflammation, mitochondrial function, and oxidative stress in a rat model. To this end, we supplemented for 4 wk the diet of male Wistar rats with HFM and with an isocaloric amount (82 kJ, 22 mL/d) of milk obtained from cows fed a diet with low forage:concentrate ratio, and analyzed the metabolic parameters of the animals. Our results indicate that HFM may positively affect lipid metabolism, leptin:adiponectin ratio, inflammation, mitochondrial function, and oxidative stress, providing the first evidence of the beneficial effects of HFM on rat metabolism.     

On the way to plastic computation

The development of post silicon technologies based on organic materials consolidates the possibility to realize new devices and applications with unusual properties: flexibility, lightweight, disposability. Both materials and processes play a fundamental role in this new electronic framework and have been improved continuously in the last decades. In this contribution, a new perspective will be drawn by considering a complete technology platform that lead printed organic electronics technology from the basic device and materials to a manufacturing process flow, design tools and market applications development. The final goal of the proposed approach is the manufacturing of organic circuits with sub-micron feature size at low fabrication costs with high flexibility and application versatility by using additive manufacturing processes. The identification of suitable material features and process steps and the implementation of dedicated CAD tools in a complete workflow are here reported. Moreover, the feasibility of the adopted technology is demonstrated by the design of both digital and analog circuits. Multilayered structure devices, like Organic Thin Film Transistor (OTFT), are used to design complex architectures like arithmetic logic units and nonlinear oscillators.     

达涅利薄板坯连铸连轧设备最新技术成果

给出达涅利薄板坯连铸连轧技术的最新应用成果.这些成果表明,达涅利技术不仅用于生产普通钢种,还可用于生产质量要求严格的优质钢种和高附加值产品.由达涅利在尼兹尼.诺夫戈罗德区Vyksa建设1套CRC(连铸连轧)设备.这是在俄罗斯建设的第1套薄板坯连铸连轧设备,也是世界上第1套利用薄板坯连铸连轧工艺路线生产北极高寒地区用API管线钢的生产设备.另外2套设备已在中国唐山钢铁集团有限公司和本溪钢铁集团有限公司进入满负荷生产阶段,前者一直保持着设备生产能力世界纪录,后者则成为中国第一家利用薄板坯连铸连轧工艺生产硅钢的厂家.     

Acute lesions of the ventromedial hypothalamus reduce sympathetic activation and thermogenic changes induced by PGE1

Modern research into the needs of intensive care unit visitors was pioneered by Molter in 1979. Much of the published research does not build on the methodology developed by Molter but simply repeats her work with minor modifications. Repetition does not always provide new information and may simply replicate methodological shortcomings. Methods of improving research in the area are described and future directions suggested.     

Experimental ocular surgery with a high-repetition-rate erbium:YAG laser

PURPOSE: To evaluate the performance in ocular surgery and the ocular tissue interactions resulting from increasing the maximum repetition rate of a pulsed-mode erbium:YAG laser system from 30 to 200 pulses per second. METHODS: An erbium:YAG laser was used that emitted at 2.94 microm with an output graduated from 0.2 mJ to 25 mJ and a repetition rate from 2 Hz to 200 Hz and that was equipped with a flexible optical fiber attached to various interchangeable 20-gauge endoprobes to perform ocular surgery in enucleated pig eyes. The specific maneuvers were performed in close contact in nontransmitting aqueous media and included inner retinal ablation, retinotomy, lens capsulotomy, lens ablation, iridotomy, and iridectomy. Selected tissue specimens were examined by light microscopy. RESULTS: Increasing the repetition rate to the 200-Hz range significantly improved the smoothness, continuity, and speed of all surgical maneuvers. Compared with the 30-Hz rate, substantially lower energies per pulse were efficient with the 200-Hz rate. The "sticking effect" between the tip of the probe and the target tissue at low-repetition rates, which resulted in discontinuation of the surgical maneuver, particularly during lens surgery, was eliminated with the use of high-repetition rates. Use of high-repetition rates produced a zone of residual thermal damage less than 30 microm in all ocular tissues. The histologic findings of tissue interactions were comparable to those obtained in published studies in which the same wavelength and low hertz rates were used. CONCLUSIONS: The high-repetition-rate erbium:YAG laser technology described is advantageous, compared with low-repetition-rate erbium:YAG lasers, and is applicable in a variety of ocular surgical procedures. Innovations in endoprobe design and further study will determine its role in contemporary ocular surgery.     

Factor-GMM estimation with large sets of possibly weak instruments

The use of factor analysis for instrumental variable estimation when the number of instruments tends to infinity is analysed. In particular, the focus is on situations where many weak instruments exist and/or the factor structure is weak. Theoretical results, simulation experiments and empirical applications highlight the relevance of Factor-GMM estimation, which is also easily implemented.     

Time-scale transformations of discrete time processes

Abstract.  This paper investigates the effects of temporal aggregation when the aggregation frequency is variable and possibly stochastic. The results that we report include, as a particular case, the well-known results on fixed-interval aggregation, such as when monthly data are aggregated into quarters. A variable aggregation frequency implies that the aggregated process will exhibit time-varying parameters and non-spherical disturbances, even when these characteristics are absent from the original model. Consequently, we develop methods for specification and estimation of the aggregate models and show with an example how these methods perform in practice.     

Pooling‐Based Data Interpolation and Backdating

Abstract. Pooling forecasts obtained from different procedures typically reduces the mean square forecast error and more generally improve the quality of the forecast. In this paper, we evaluate whether pooling‐interpolated or‐backdated time series obtained from different procedures can also improve the quality of the generated data. Both simulation results and empirical analyses with macroeconomic time series indicate that pooling plays a positive and important role in this context also.     

Combining mass spectrometry and pull-down techniques for the study of receptor heteromerization. Direct epitope-epitope electrostatic interactions between adenosine A2A and dopamine D2 receptors

Previous results from FRET and BRET experiments and computational analysis (docking simulations) have suggested that a portion of the third intracellular loop (I3) of the human dopamine D2 receptor (D2R) and the C-tail from the human adenosine A2A receptor (A2AR) are involved in A2AR-D2R heteromerization. The results of the present studies, using pull-down and mass spectrometry experiments, suggest that A2AR-D2R heteromerization depends on an electrostatic interaction between an Arg-rich epitope from the I3 of the D2R (217RRRRKR222) and two adjacent Asp residues (DD401-402) or a phosphorylated Ser (S374) residue in the C-tail of the A2AR. A GST-fusion protein containing the C-terminal domain of the A2AR (GST-A2ACT) was able to pull down the whole D2R solubilized from D2R-tranfected HEK-293 cells. Second, a peptide corresponding to the Arg-rich I3 region of the D2R (215VLRRRRKRVN224) and bound to Sepharose was able to pull down both GST-A2ACT and the whole A2AR solubilized from A2AR-tranfected HEK-293 cells. Finally, mass spectometry and pull-down data showed that the Arg-rich D2R epitope binds to two different epitopes from the C-terminal part of the A2AR, containing the two adjacent Asp residues or the phosphorylated Ser residue (388HELKGVCPEPPGLDDPLAQDGAVGS412 and 370SAQEpSQGNT378). The present results are the first example of epitope-epitope electrostatic interaction underlying receptor heteromerization, a new, expanding area of protein-protein interactions.     

A parametric estimation method for dynamic factor models of large dimensions

Abstract.  The estimation of dynamic factor models for large sets of variables has attracted considerable attention recently, because of the increased availability of large data sets. In this article we propose a new parametric methodology for estimating factors from large data sets based on state–space models and discuss its theoretical properties. In particular, we show that it is possible to estimate consistently the factor space. We also conduct a set of simulation experiments that show that our approach compares well with existing alternatives.