B Cells with a Senescent-Associated Secretory Phenotype Accumulate in the Adipose Tissue of Individuals with Obesity

Senescent cells accumulate in the adipose tissue (AT) of individuals with obesity and secrete multiple factors that constitute the senescence-associated secretory phenotype (SASP). This paper aimed at the identification of B cells with a SASP phenotype in the AT, as compared to the peripheral blood, of individuals with obesity. Our results show increased expression of SASP markers in AT versus blood B cells, a phenotype associated with a hyper-metabolic profile necessary to support the increased immune activation of AT-derived B cells as compared to blood-derived B cells. This hyper-metabolic profile is needed for the secretion of the pro-inflammatory mediators (cytokines, chemokines, micro-RNAs) that fuel local and systemic inflammation.     

Compositional characteristics,texture, shelf-life and sensory quality of snack crackers produced from non-traditional ingredients

The study was conducted to evaluate the effect of non-traditional and highly available and nutrient-dense ingredients in the production of value-added crackers. The crackers were prepared by combining partially defatted chia flour, wheat germ, quinoa, and oat. The antioxidant activity and the polyphenol content were 7.0–8.4 times higher in crackers produced with non-traditional ingredients compared to the control snack. The hydroperoxide value indicated a low oxidative deterioration of the oil. The sample with 10% of partially defatted chia flour was selected for shelf-life test under storage conditions and sensory evaluation. Modified atmosphere exerted a protective effect on the lipid stability regardless of the incorporation of BHT to the formulation. All the evaluated attributes scored highly during consumer acceptance. The formulated cracker presented a relevant content of protein, dietary fibre and omega-3 and -6 fatty acids. Based on these results, the crackers containing non-traditional ingredients resulted in a product with a good potential for both consumer acceptance and outstanding nutritional benefits.     

Cell Death Mechanisms Induced by CLytA-DAAO Chimeric Enzyme in Human Tumor Cell Lines

The combination of the choline binding domain of the amidase N-acetylmuramoyl-L-alanine (CLytA)-D-amino acid oxidase (DAAO) (CLytA-DAAO) and D-Alanine induces cell death in several pancreatic and colorectal carcinoma and glioblastoma cell lines. In glioblastoma cell lines, CLytA-DAAO-induced cell death was inhibited by a pan-caspase inhibitor, suggesting a classical apoptotic cell death. Meanwhile, the cell death induced in pancreatic and colon carcinoma cell lines is some type of programmed necrosis. In this article, we studied the mechanisms that trigger CLytA-DAAO-induced cell death in pancreatic and colorectal carcinoma and glioblastoma cell lines and we acquire a further insight into the necrotic cell death induced in pancreatic and colorectal carcinoma cell lines. We have analyzed the intracellular calcium mobilization, mitochondrial membrane potential, PARP-1 participation and AIF translocation. Although the mitochondrial membrane depolarization plays a crucial role, our results suggest that CLytA-DAAO-induced cell death is context dependent. We have previously detected pancreatic and colorectal carcinoma cell lines (Hs766T and HT-29, respectively) that were resistant to CLytA-DAAO-induced cell death. In this study, we have examined the putative mechanism underlying the resistance in these cell lines, evaluating both detoxification mechanisms and the inflammatory and survival responses. Overall, our results provide a better understanding on the cell death mechanism induced by CLytA-DAAO, a promising therapy against cancer.     

Price competition between a macrocell and a small-cell service provider with limited resources and optimal bandwidth user subscription: a game-theoretical model

The ever-increasing demand for higher data rates in wireless commutations provides a rationale for small cells deployment. While the physical and technological aspects of small-cell networks have been extensively studied in recent years, the economic analysis has received much less attention. We focus on the economic rationale for a small-cell service provider (SSP) operating a market where an incumbent macrocell service provider (MSP) exists, and competition develops. We analyze such scenario for the case of fixed users by means of Game Theory, specifically through a two-stage game: in the first stage each service provider posts its price according to a Stackelberg game where the MSP is the leader and the SSP is the follower; and, in the second stage, each user chooses both which provider to subscribe to and the optimal amount of bandwidth. A subgame perfect Nash equilibrium is used as a solution concept, and it is derived analytically. We show that the SSP has an incentive to operate in the market and its profit gets higher as SSP’s resources increase. Furthermore, users benefit from SSP’s operation, which may provide a rationale for a regulatory authority to grant the SSP access to the market, despite the fact that MSP’s profit is harmed. Finally, we identify two modes of operation of the system, which depend on the SSP coverage: one where SSP’s deployment is limited and the MSP strategy is not affected by SSP competition and takes only the users outside the SSP coverage; and another, where the SSP covers a large area and the MSP competes against the SSP taking a fraction of the users inside the small cells.     

A good practice for making training accessible to university faculty members through ICTs: syllabus planning support training

Universal Access in the Information Society - This study focuses on a case study developed at a higher education institution, which comprises developing a new virtual teaching unit (VTU) aimed at...