Ordinary Gasoline Emissions Induce a Toxic Response in Bronchial Cells Grown at Air-Liquid Interface

Gasoline engine emissions have been classified as possibly carcinogenic to humans and represent a significant health risk. In this study, we used MucilAir™, a three-dimensional (3D) model of the human airway, and BEAS-2B, cells originating from the human bronchial epithelium, grown at the air-liquid interface to assess the toxicity of ordinary gasoline exhaust produced by a direct injection spark ignition engine. The transepithelial electrical resistance (TEER), production of mucin, and lactate dehydrogenase (LDH) and adenylate kinase (AK) activities were analyzed after one day and five days of exposure. The induction of double-stranded DNA breaks was measured by the detection of histone H2AX phosphorylation. Next-generation sequencing was used to analyze the modulation of expression of the relevant 370 genes. The exposure to gasoline emissions affected the integrity, as well as LDH and AK leakage in the 3D model, particularly after longer exposure periods. Mucin production was mostly decreased with the exception of longer BEAS-2B treatment, for which a significant increase was detected. DNA damage was detected after five days of exposure in the 3D model, but not in BEAS-2B cells. The expression of CYP1A1 and GSTA3 was modulated in MucilAir™ tissues after 5 days of treatment. In BEAS-2B cells, the expression of 39 mRNAs was affected after short exposure, most of them were upregulated. The five days of exposure modulated the expression of 11 genes in this cell line. In conclusion, the ordinary gasoline emissions induced a toxic response in MucilAir™. In BEAS-2B cells, the biological response was less pronounced, mostly limited to gene expression changes.     

Synthesis and Characterization of Hetero-metallic Oxides-Reduced Graphene Oxide Nanocomposites for Photocatalytic Applications

A new AgO.CuO.WO₃/rGO nanocomposite was designed for the investigation of the degradation ability of the hybrid material under visible light irradiation. The AgO, CuO, WO₃ NPs, and AgO.CuO.WO₃ hetero-metallic oxides were fabricated via the chemical co-precipitation method. The crystallite sizes and phase analyses were investigated by recording X-ray diffraction patterns. The crystallite sizes of three metal oxides in the AgO.CuO.WO₃ hetero metal oxide were 16.7, 15.9, and 16.9 nm, respectively. The FESEM images at various magnifications were probed to study the morphology of synthesized materials. The micrographs of hetero-metallic oxides AgO.CuO.WO₃ exposed that three metal oxides merged like small particles and gives a large bulbous appearance. EDX analyses confirmed the formation of required materials with high purity. FTIR data was in agreement with the literature which facilitated to ensure the purity of synthesized samples. The optical bandgap energy was calculated via the Tauc plot indicating that the blend of three metal oxides generated a new energy level in the electronic structure is suitable for photocatalysis in the presence of visible light. The bandgap energy of hetero metallic oxides was 1.25 eV which is less than individual metal oxides signifying the tuning of the bandgap. The incorporation of rGO in AgO.CuO.WO₃ hetero-metallic oxides gives a new photocatalyst for optimum photodegradation of methylene blue in minimum time. The percentage degradation via AgO.CuO.WO₃ was 87.20% in 70 min while the percentage degradation via AgO.CuO.WO₃/rGO recorded by photocatalytic experiment was 95% in 40 min. The photocatalysis data revealed that AgO.CuO.WO₃ hetero-metallic oxides-rGO nanocomposite ensured a strong potential to uptake organic dyes from water by promoting redox reactions during photocatalysis in the minimum time limit.     

Ternary Blends of Renewable Fast Pyrolysis Bio-Oil,Advanced Bioethanol,and Marine Gasoil as Potential Marine Biofuel

An alternative for reducing emissions from marine fuel is to blend bio-oil from lignocellulose non-edible feedstocks to diesel fossil fuels. Phase diagrams of the ternary systems were built to represent the transition from heterogeneous regions to homogeneous regions. Four homogeneous blends of bio-oil of eucalyptus-bioethanol-marine gasoil were experimentally characterized with respect to the most important fuel parameters for marine engines: water content, flash point, low heating value, viscosity, and acidity. Blends with closer properties to marine gasoil replacement, lower costs, and environmental impacts should be tested for large engines.     

Influence of different viscosity grade cellulose-based polymers on the development of valsartan controlled release tablets

This work is based on formulating and optimizing controlled release (CR) valsartan (160 mg) tablets using different viscosity grades of the cellulosic polymer. The objective was to develop an effective once-daily drug delivery system of this cardiovascular agent. Central composite design was used for designing the formulations. Polymers used were Methocel® K4M, K15M and K100M. Compatibility of excipients with active was studied through FT-IR. Micromeritic properties were determined and formulations exhibiting appropriate flow characteristics were compressed. Swelling behavior and in vitro buoyancy effect were studied and response surface curves were constructed to optimize the formulation. Multi-point dissolution profiles of valsartan CR tablets at pH 1.2, 4.5 and 6.8 were obtained. Model-dependent and model-independent methods were performed including f2, stability test as per ICH guidelines and ANOVA. FT-IR studies revealed the compatibility of valsartan with all excipients. Formulation K4T9 (containing 25% K4M polymer) was selected to be the best optimized trial, based on physical properties and controlled release profile (23% at 4 h, 82% at 16 h and 100% at 24 h). Results of buoyancy and swelling behavior indicated that HPMC-K4M polymer exhibited excellent floating lag time and swelling indexes. In vitro drug release kinetics showed that formulation K4T9 displayed Korsmeyer–Peppas drug release pattern with r value > 0.99. The manufacturing process of K4T9 was also found to be reproducible with a shelf life period of 41 and 36 months at room temperature and accelerated conditions, respectively. Valsartan CR matrix-based formulation was successfully prepared with Methocel K4M retardant.     

Influence of moisture content on the contact angle and surface tension measured on birch wood surfaces

European Journal of Wood and Wood Products - Surface tension of solid wood surfaces affects the wettability and thus the adhesion of various adhesives and wood coatings. By measuring the contact...     

Electronic,Magnetic, Electric and Magnetocaloric Properties of the Multiferroic Orthorhombic YMnO3

Journal of Superconductivity and Novel Magnetism - Multiferroic oxide materials have attracted much intention in recent years due to their application in different fields such as magnetic...     

Inversion of three-stage stress-strain relation for stainless steel in tension and compression

Presented in this paper is a new stress-strain relation for stainless steel alloys that provides the stress as an explicit function of the strain. The relation is an approximate inversion of a recently proposed three-stage stress-strain relation based on a modified Ramberg-Osgood equation. The three-stage relation is a much more accurate formulation than the previous two-stage formulations and is applicable to both tensile as well as compressive stresses. The new relation is derived by making a rational function assumption on the fractional deviation of the actual stress-strain curve from an idealized linear elastic behaviour. The new expression is valid over the full range of the stress well beyond the elastic region. The validity of the inverted expression is tested over a wide range of material parameters. These tests demonstrate that, the new expression results in stress-strain curves which are both qualitatively and quantitatively in excellent agreement with the fully iterated numerical solution of the full-range stress-strain relation with a maximum error below 4%.     

Optimization approaches and climates investigations in NZEB—A review

The conception of net zero energy buildings (NZEB) has been introduced to limit energy consumption and pollution emissions in buildings. Classification of NZEB is based on renewable energy (RE) supply options, energy measurement process, RE-sources location, and balances whether are energetic or exergetic. In general, it is traditionally agreed that there are three main steps to reach the NZEB performance, starting through the use of passive strategies, energy efficient technologies, and then RE generation systems. Then, these three steps could be accompanied with the smart integration of advanced efficient energy technologies. A state of the art shows that the main ZEB studies are related to: energy savings, reduce electric bills, energy independence, pollution reduction, and occupants comfort, in addition, others are more interested in the aesthetic aspect by combining modern technologies with innovations to achieve high energy and sustainability performance. Building optimization is a promising technique to evaluate NZEB design choices; it has been adopted to choose the perfect solution to reach the zero energy performance through the optimization of an objective function related to energy (thermal loads, RE generation, energy savings) and/or environment (CO₂ emissions) and/or economy (life-cycle cost (LCC), net-present value (NPV), investment cost). This paper starts by presenting the global energetic and pollution challenges the world faces. Moreover, it shows, to the best to the author’s knowledge, the existing NZEB definitions and the corresponding case studies investigated in 8 different climatic zones (humid continental, humid subtropical, Mediterranean, moderate continental, moderate continental, marine west coast, tropical, semi-arid and hot), the paper also focus on the importance to treat each climate separately. Even in the same country, two or more climates may co-exist. NZEBs drawbacks are also presented. Furthermore, different optimization problems are reviewed in the last section. Building energy optimization methods are employed to obtain the ideal solution for specific objective functions which are either related to energy, and/or environment and/or economy. Optimization variables are distributed between passive and/or RE generation systems. Finally, a table summarizing the most commonly used electric and thermal RE applications which yield to the zero energy balance in each climate, as well as three flowcharts are presented to summarize the whole three-stage procedure, to reach NZEB, starting from building designing, passing through the optimization procedure, and lastly categorizing the zero energy balance.