Coralline Skeleton Biomaterial Reduces Phagocytosis in Mouse Blood in vitro

Inflammatory and immunogenic response to foreign bodies presents a challenge in the use of biomaterials as implants for tissue restoration. Therefore, there is a need to understand the interactions between such implants and the blood. One such material, currently in clinical use for bone replacement in humans, is the skeleton of corals, in the form of crystalline aragonite. This biomaterial has been shown to impart a protective and supportive influence on several types of cells ex vivo. The carbonate skeleton activates secretion in phagocytes in vitro, however its effects on these cells in the blood, and on the process of phagocytosis itself, remain unknown. Using 1–500 μm particles of coral skeleton, we show that these particles bind blood proteins and alter the leukocyte population, reducing the proportion of granulocytes by more than 3-fold with no effect on the proportion of monocytes. In addition, the presence of coral skeleton in the blood causes a reduction in phagocytosis. Specifically, we observed a decrease in the percentage of phagocytic cells by 27 % in the granulocytes and by 73 % in monocyte family, as well as a 41.6 % reduction in the MFI of granulocytes, but with no such effect on monocytes. Taken together, the results suggest that the coral skeleton biomaterial may act as a strong, promotive scaffold for tissue regeneration due to its ability to reduce its rejection by inflammatory reactions such as phagocytosis.     

The Use of Fractional Factorial Design for Atrium Fires Prediction

In order to mitigate the excessive computational cost of atrium fire simulations, a novel methodology based on the use of the Fractional Factorial Design technique to obtain an experimental validated tool, in the form of a surface response model, capable to predict fire induced conditions is proposed. This methodology is supported by results from a Design of Experiments benchmark, which consists of a set of FDS simulations in the present work. Specifically, a \(2^{6-2}_{IV}\) approach has been considered and applied to a 20 m cubic atrium. Thus, six factors have been considered, namely the fire Heat Release Rate (HRR) and location, the exhaust flow rate, the exhaust location and activation time, and the inlet vents area. Furthermore, the smoke temperature at the roof and 15 m high and the smoke layer height have been considered the variables of interest. Subsequently, a multiple linear regression analysis has been performed to predict and compare the steady and non-steady temperature profiles and the smoke layer drop with six novel full-scale atrium fire tests, and also with specific adjusted FDS models. In addition, this methodology has been extended successfully to predict the non-steady behaviour of the fire tests. At the steady state, the HRR and the exhaust flow rate have been found to be the most relevant factors. The results obtained with the proposed methodology show a good fit both with the fire tests and with the adjusted FDS models, with discrepancies mostly below 14%. For non-steady conditions, a time analysis of the influence of the six factors has been carried out. Again, remarkable good agreement with the time-dependent experimental results is achieved, with average discrepancies below 12%, being the larger differences found in the prediction of local effects, such as the smoke ceiling jet, for high HRR or when the make-up air influence is significant. The results turn this methodology into a powerful and useful tool for fire safety designs.     

A review on some classes of algebraic systems

ABSTRACT

In this paper, we review some algebraic control system. Precisely, linear and bilinear systems on Euclidean spaces and invariant and linear systems on Lie groups. The fourth classes of systems have a common issue: to any class, there exists an associated subgroup. From this object, we survey the controllability property. Especially, from those coming from our contribution to the theory.     

Order/Disorder in Protein and Peptide-Based Biomaterials

Nature utilizes both order and disorder (or controlled disorder) to achieve exceptional materials properties and functions, while synthetic supramolecular materials mostly exploit just supramolecular order, thus limiting the structural diversity, responsiveness and consequent adaptive functions that can be accessed. Herein, we review the emerging field of supramolecular biomaterials where disorder and order deliberately co-exist, and can be dynamically regulated by considering both entropic and enthalpic factors in design. We focus on sequence-structure relationships that govern the (cooperative) assembly pathways of protein and peptide building blocks in these materials. Increasingly, there is an interest in introducing dynamic features in protein and peptide-based structures, such as the remarkable thermo-responsiveness and exceptional mechanical properties of elastin materials. Simultaneously, advances in the field of intrinsically disordered proteins (IDPs) give new insights about their involvement in intracellular liquid-liquid phase separation and formation of disordered, dynamic coacervate structures. These have inspired efforts to design biomaterials with similar dynamic properties. These hybrid ordered/disordered materials employ a combination of intramolecular and supramolecular order/disorder features for construction of assemblies that are dynamically reconfigurable. The assembly of these dynamic structures is mainly entropy-driven, relying on electrostatic and hydrophobic interactions and is mediated in part through the adopted (unstructured) protein conformation or by introducing an oppositely charged guest for peptide building blocks. Examples include design of protein building blocks composed of disordered repeat sequences of elastin-like polypeptides in combination with ordered regions that adopt a secondary structure, the co-assembly of proteins with peptide amphiphiles to achieve reconfigurable, yet highly stable membranes or tyrosine-containing tripeptides with sequence-controlled order/disorder that upon enzymatic oxidation give rise to melanin-like polymeric pigments with customizable properties. The resulting hybrid materials with controlled disorder can be metastable, and sensitive to various external stimuli giving rise to insights that are especially attractive for the design of responsive and adaptive materials.     

An X-ray absorption approach to mixed and metallicity-sorted single-walled carbon nanotubes

Carbon based structures have been widely studied by X-ray absorption (XAS), also called NEXAFS, which is a very useful bulk probing method that allows examining the unoccupied density of states (DOS) and the site selective bonding environment. Two very well known spectral features in the XAS core level spectrum are the σ* and π* bands, and both have been analyzed in several studies for graphitic-like systems. However, among all the carbon materials, the unique one-dimensional electronic properties attributed to single-walled carbon nanotubes (SWCNTs) exhibit features that reveal clearly their electronic structure in the core level XAS spectrum. In this article, we outline the C1s response in XAS, which is related to the DOS of the conduction band in SWCNTs and its fine structure, revealed by experiments performed on metallicity-sorted SWCNT material. The progress in the identification of changes in the site selective conduction band electronic structure with XAS is discussed in detail.     

Perfect discrete Morse functions on 2-complexes

This paper is focused on the study of perfect discrete Morse functions on a 2-simplicial complex. These are those discrete Morse functions such that the number of critical i-simplices coincides with the ith Betti number of the complex. In particular, we establish conditions under which a 2-complex admits a perfect discrete Morse function and conversely, we get topological properties necessary for a 2-complex admitting such kind of functions. This approach is more general than the known results in the literature (Lewiner et al., 2003), since our study is not restricted to surfaces. These results can be considered as a first step in the study of perfect discrete Morse functions on 3-manifolds.     

Muscle tissue structural changes and texture development in sea bream, Sparus aurata L., during post-mortem storage

Sea bream, Sparus aurata L., specimens were studied in pre-rigor (3 h) and during the following post-mortem days: 1, 5, 10, 15 and 22. Muscle and textural parameters were evaluated on 6 specimens/stage. Structural results showed scarce fibre-to-fibre detachment on pre-rigor, which increased during the post-mortem degradation. Ultrastructural changes revealed rapid muscle degradation. In pre-rigor myofibrils were detached to both sarcolemma and endomysium. Intermyofibrillar spaces increased and some mitochondriae and sarcoplasmic reticulum were swollen. After 1 day, the sarcolemma appeared occasionally disrupted and the interfibrillar spaces increased. From 5 to 10 days, the I-band and Z line presented some alterations, although these were more severe at 15-22 days. Thus, in these two last stages, loss of I-band, Z line and actin filaments was observed, that coincides with the alteration of the hexagonal arrangement in these advanced stages. Also, the fragmentation of myofibrils increased from 5 to 10 days on. Sarcolemma and endomysium were gradually disrupted throughout the post-mortem stages with total loss at 22 days. Consequently, the interfibrillar spaces increased at last stages. Autophagic mechanisms increased from 5 days on, with an intense destruction of all the intracytoplasmic organelles. Textural parameters decreased from pre-rigor until 5-10 days, mainly associated to detachment of myofibers to sarcolemma-endomysium.