The repair of bone fractures is a clinical challenge for patients with impaired healing, such as osteoporosis. Currently, different strategies have been developed to design new biomaterials, enhancing their interactions with biological systems and conducting the cellular behavior in the desired direction to help fracture healing. In the present work, hydroxyapatite-graphene oxide (HA-GO) nanocomposites were produced and the morphological and physicochemical influences of the addition of 0.5 wt%, 1.0 wt% and 1.5 wt% of GO to HA were observed. FEG-SEM and TEM analyses of HA-GO nanocomposites showed HA nanoparticles adhered to the surface of the GO sheets, suggesting an effective method to form nanostructured graphene-based biomaterials. As confirmation, physicochemical analyses by Raman, FTIR and TGA demonstrated a strong affinity between HA and GO, according to the increase of concentration from 0.5 wt% to 1.5 wt% GO in the HA-GO nanocomposites. Also, in order to evaluate the HA-GO nanocomposites behavior under biological microenvironment, in vitro bioactivity and indirect cytotoxicity tests were performed. FEG-SEM analyses confirmed the positive results for the bioactivity properties of HA-GO nanocomposite and indirect cytotoxicity demonstrated that even with a decrease in the hDPSCs viability and proliferation, when increasing to 1.5 wt% of GO concentration, high level of cell viability was exhibited by HA-GO nanocomposites. These biological results suggested the 0.5 wt% HA-GO nanocomposite as a potential bioactive bone graft and a promising biomaterial for bone tissue regeneration, when compared to the pure HA. 相似文献
This article examines the absolute and conditional convergence of real GDP per capita in the Common Market for Eastern and Southern Africa (COMESA) during the period 1950?C2003. Income departures across countries were evaluated from several panel data unit root tests. We find no evidence supporting the existence of convergence process for the income in the COMESA. Nevertheless, applying economic development criterion allows to identity two absolute convergence clubs into the COMESA, one for the most four developed countries (Egypt, Libya, Mauritius, Seychelles), and one other for the fourteen less developed ones. Thus, we show that most economies of COMESA are locked into a sustained poverty trap process. 相似文献
Liking for a sweet, salty or fatty diet may induce overconsumption of simple carbohydrates, sodium or lipids. Measuring overall liking of the corresponding sensory sensations contributes to understanding the determinants of dietary behaviours. However, no standardized validated questionnaire assaying these sensations is currently available. In the present study, we developed a web-based questionnaire, “PrefQuest”, which measures recalled liking for the following four sensations: salt, fat and salt, sweet, fat and sweet. PrefQuest included four types of items: (1) liking for sweet, fatty-sweet and fatty-salty foods, (2) preferences in the level of seasoning by adding salt, sweeteners, or fat, (3) preferences for types of dishes in a restaurant menu and (4) overall questions about sweet-, salt- and fat-related behaviours. A development study (n = 198) demonstrated that PrefQuest was feasible, well-perceived, only lasted about 20 min and that items were repeatable (overall mean intra-class correlation coefficient: 0.77, SD 0.08). Construct validity was then evaluated on a larger population sample (n = 47 803). The underlying structure within each of the four sensations was determined by exploratory factor analysis and then internally validated by confirmatory factor analysis. After a selection of the most relevant items, the salt, fat-and-salt, sweet, and fat-and-sweet scales exhibited a theoretically meaningful factor structure, unidimensional for the salt scale and with interrelated sub-dimensions for the sweet, fat-and-salt, and fat-and-sweet scales. Based on the fat-and-sweet and fat-and-salt scales, a fat model was also unveiled. For each factor, internal consistency as well as convergent and divergent validities were demonstrated. Overall, PrefQuest is an internally valid and original tool that can be used to assay recalled liking for sweet, salt, and fat considered altogether or separately as fat and salt or fat and sweet, and can be applied to large population surveys. 相似文献
Objective: The aim of this study was to evaluate a formulation made of poly(lactide-co-glycolide) (PLGA) nanoparticles containing azelaic acid for potential acne treatment.
Methods: Azelaic acid-loaded PLGA nanoparticles were prepared by spontaneous emulsification processes using poloxamer 188 as stabilizer. Several manufacturing parameters such as stirring rate, concentration of stabilizer and different recovery methods were investigated. Nanoparticles were evaluated in terms of size, zeta potential, encapsulation efficiency, release kinetics and permeation kinetics in vitro. Furthermore, in vitro toxicological studies were performed in Saccharomyces cerevisiae model.
Results: The results showed that by adjusting some formulation conditions it was possible to obtain nanoparticles with high loading and a controlled drug release. Freeze-dried recovery altered the nanoparticles structure by enhancing porous structures and mannitol was required to control the mean particle size. The centrifugation recovery was found to be the best approach to nanoparticles recovery. Similar toxicity profiles were observed for both drug-free and azelaic acid-loaded nanoparticles, with concentration-dependent decreases in cell viability.
Conclusion: These results indicate a potential formulation for controlled release delivery of azelaic acid to the follicular unit. 相似文献
Although land managers and policy-makers generally have a good experience of what result can be expected from their decisions,
they are often faced with difficulty when trying to communicate the visual impact of a management option to stakeholders,
particularly when the landscape exhibits a high cultural value. Three-dimensional visualization of the landscape is often
used for communicating with the stakeholders. A challenge in participatory methods for integrated assessment and policy planning
is to view future changes in land use, according to scenarios. A 3-D landscape visualization component, SLE (“Seamless Landscape
Explorer”), has been developed, which is launched after a scenario simulation to allow for exploration of landscape changes.
Pressures causing such changes are translated into changes in the spatial configuration of the landscape. The different types
of land-use are visualized thanks to a library of detailed textures, and vegetation can be added. This has been applied to
a study of four scenarios in the French Mediterranean region, which were set up as part of a participatory process for discussing
the planning of the regional peri-urban and agricultural policy, in an area dominated by the typical culturally sensitive
Mediterranean matorral, (“garrigue” shrubland) surrounding the Pic Saint-Loup mountain. Examples of visualization are shown
and discussed here. 相似文献
Screen-printed n+–p–p+ solar cells were fabricated on Cz single crystalline Si material, with a 45 Ω/sq emitter and PECVD SiNx antireflective coating with a thickness of 700 Å, using different Ag pastes and commercial leaded reference paste (CN33-462, Ferro Corp.). Ag and Al contacts were co-fired using a mass-production line equipped with mesh belt conveyer furnace systems (Centrotherm thermal solution GmbH & Co. KG). The average results for single crystalline Si solar cells (156 cm2) are: Isc=5.043 A, Voc=0.621 V, Rs=0.0087 Ω, Rsh=15.3 Ω, FF=0.773, and Eff=16.45%. Rsh and fill factor values of fabricated cells were slightly higher when compared with the commercial leaded Ag paste, although cells were fabricated by metallizing the lead-free silver pastes. For the lead-free Ag paste used in this study, the line pattern continuity is retained with improved edge definition in sharp contrast to that of reference Ag paste. Average value of Rs was also equivalent approximately to that of the leaded Ag paste. 相似文献