某些变色酸偶氮类染料与蛋白质作用的分光光度研究

用分光光度法研究并比较了１０种变色酸偶氮染料（偶氮胂Ⅰ、偶氮胂Ⅲ、偶氮氯膦Ⅰ、偶氮氯膦Ⅲ、氨基Ｃ酸偶氮胂、氨基Ｇ酸偶氮氯膦、邻溴偶氮氯膦、间碘偶氮氯膦、二溴乙氧基偶氮氯膦、邻溴偶氮胂）与蛋白质的作用及所引起的光谱性能变化。详细探讨了染料结构、缓冲条件、种类及酸度对染料与蛋白质作用的影响,用双波长法测定了最大结合数及结合常数。     

Homogeneous detection of human IgG by gold nanoparticle probes

A simple, and homogeneous detection system for human IgG based on the optical properties of aggregated gold nanoparticles probes were investigated. When gold nanoparticles with about 13 nm in diameter were modified by goat anti-human IgG, the addition of human IgG could change the absorption of colloidal gold solution, and the absorption intensity at 740 nm depended on the amount of human IgG. The aggregation of gold nanoparticles was also validated using transmission electron microscopy (TEM). A series of experiments were carried out to study the effects of pH value, the reaction temperature, and non-specific adsorption on the assay. A dynamic range of 10–500 μg/3 mL human IgG was observed. The new bioassay could be used for the rapid and homogeneous detection of antibodies in bioanalytical chemistry.