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排序方式: 共有136条查询结果,搜索用时 15 毫秒
1.
Charles H. A. Snijders Rob A. Samson Ellen S. Hoekstra Thérèse Quellet J. David Miller Petra C. E. M. de Rooij-van der Goes A. J. M. Baar A. E. J. Dubois Henk F. Kauffman 《Mycopathologia》1996,135(2):119-128
In the European coastal dunes, marram grass (Ammophila arenaria) is planted in order to control sand erosion. In the years 1986 to 1991, workers on the Wadden islands in the Netherlands planting marram grass showed lesions of skin and mucous membranes, suggesting a toxic reaction. Fusarium culmorum dominated the mycoflora of those marram grass culms that were used for planting. This plant material had been cut and stored for more than one week in the open. The Fusarium toxin deoxynivalenol (DON) was detected in the suspect marram grass culms. Isolated F. culmorum strains were able to produce DON in vitro in liquid culture as well as in experimentally inoculated wheat heads. Pathogenicity tests, toxin test as well as RAPD analysis showed that the F. culmorum strains were not specialized for marram grass but may form part of the West-European F. culmorum population infecting cereals and grasses. Storage on old sand-dunes with plant debris may have led to the high occurrence of F. culmorum and contamination with DON. Marram grass culms should be obtained from young plantings on dunes on the seaward slopes and cut culms should not be stored. 相似文献
2.
Christopher A Lamb Stefanie Nühlen Delphine Judith David Frith Ambrosius P Snijders Christian Behrends Sharon A Tooze 《The EMBO journal》2016,35(3):281-301
Macroautophagy requires membrane trafficking and remodelling to form the autophagosome and deliver its contents to lysosomes for degradation. We have previously identified the TBC domain‐containing protein, TBC1D14, as a negative regulator of autophagy that controls delivery of membranes from RAB11‐positive recycling endosomes to forming autophagosomes. In this study, we identify the TRAPP complex, a multi‐subunit tethering complex and GEF for RAB1, as an interactor of TBC1D14. TBC1D14 binds to the TRAPP complex via an N‐terminal 103 amino acid region, and overexpression of this region inhibits both autophagy and secretory traffic. TRAPPC8, the mammalian orthologue of a yeast autophagy‐specific TRAPP subunit, forms part of a mammalian TRAPPIII‐like complex and both this complex and TBC1D14 are needed for RAB1 activation. TRAPPC8 modulates autophagy and secretory trafficking and is required for TBC1D14 to bind TRAPPIII. Importantly, TBC1D14 and TRAPPIII regulate ATG9 trafficking independently of ULK1. We propose a model whereby TBC1D14 and TRAPPIII regulate a constitutive trafficking step from peripheral recycling endosomes to the early Golgi, maintaining the cycling pool of ATG9 required for initiation of autophagy. 相似文献
3.
van Deursen DL Snijders CJ van Dieën JH Kingma I van Deursen LL 《Journal of biomechanics》2001,34(3):405-408
To study the immediate effects of axial rotation on the intervertebral disc, six pig cadaver lumbar functional spinal units were exposed to rotations of up to 2 degrees, while disc height and intradiscal pressure were measured. The results showed that rotary movements are capable of causing an immediate increase in disc height and drop in nucleus pressure. However, the long-term effects were opposite in direction. 相似文献
4.
van Deursen DL Lengsfeld M Snijders CJ Evers JJ Goossens RH 《Journal of biomechanics》2000,33(6):695-699
The aim of this study was to develop a model which describes the mechanical spinal response to small alternating pelvic stimulation induced by an active rotational movement of a normal chair. The rotary continuous passive motion (RCPM) of the seat about a vertical axis of only 0.6 degrees resulted in an increased in spinal length as opposed to the normal daily shrinkage, and back patients experienced pain relief. Passive and active exercies have been broadly applied for treating and healing spinal disorders. A rigid body package (ADAMS Android) was used to translate the stimulation of the ischial tubersoity in caudo-cranial handing-over visualisation. The parameters of the model were set so that the values of the global stiffness and geometry of the intervertebral discs could be changed. In vivo validation of the model was based on force and moment measurements using an internal AO fixator. The predicitons of the model concerning natural frequency (4.5Hz) in vertical direction and the axial torsion response on small pelvic torsion are comparable with experimental data. 相似文献
5.
Interactions between elevated CO2 concentration, nitrogen and water: effects on growth and water use of six perennial plant species 总被引:2,自引:1,他引:1
Two experiments are described in which plants of six species were grown for one full season in greenhouse compartments with 350 or 560 μ mol mol–1 CO2 . In the first experiment two levels of nitrogen supply were applied to study the interaction between CO2 and nitrogen. In the second experiment two levels of water supply were added to the experimental set-up to investigate the three-way interaction between CO2 , nitrogen and water. Biomass and biomass distribution were determined at harvests, while water use and soil moisture were monitored throughout the experiments. In both experiments a positive effect of CO2 on growth was found at high nitrogen concentrations but not at low nitrogen concentrations. However, plants used much less water in the presence of low nitrogen concentrations. Drought stress increased the relative effect of elevated CO2 on growth. Available soil moisture was used more slowly at high CO2 during drought or at high nitrogen concentrations, while at low nitrogen concentrations decreased water use resulted in an increase in soil moisture. The response to the treatments was similar in all the species used. Although potentially faster growing species appeared to respond better to high CO2 when supplied with a high level of nitrogen, inherently slow-growing species were more successful at low nitrogen concentrations. 相似文献
6.
Hao Wu Lei Sun Fabian Blombach Stan J.J. Brouns Ambrosius P. L. Snijders Kristina Lorenzen Robert H. H. van den Heuvel Albert J. R. Heck Sheng Fu Xuemei Li Xuejun C. Zhang Zihe Rao John van der Oost 《Proteins》2010,78(3):705-713
The HflX‐family is a widely distributed but poorly characterized family of translation factor‐related guanosine triphosphatases (GTPases) that interact with the large ribosomal subunit. This study describes the crystal structure of HflX from Sulfolobus solfataricus solved to 2.0‐Å resolution in apo‐ and GDP‐bound forms. The enzyme displays a two‐domain architecture with a novel “HflX domain” at the N‐terminus, and a classical G‐domain at the C‐terminus. The HflX domain is composed of a four‐stranded parallel β‐sheet flanked by two α‐helices on either side, and an anti‐parallel coiled coil of two long α‐helices that lead to the G‐domain. The cleft between the two domains accommodates the nucleotide binding site as well as the switch II region, which mediates interactions between the two domains. Conformational changes of the switch regions are therefore anticipated to reposition the HflX‐domain upon GTP‐binding. Slow GTPase activity has been confirmed, with an HflX domain deletion mutant exhibiting a 24‐fold enhanced turnover rate, suggesting a regulatory role for the HflX domain. The conserved positively charged surface patches of the HflX‐domain may mediate interaction with the large ribosomal subunit. The present study provides a structural basis to uncover the functional role of this GTPases family whose function is largely unknown. Proteins 2010. © 2009 Wiley‐Liss, Inc. 相似文献
7.
Melles DC van Leeuwen WB Snijders SV Horst-Kreft D Peeters JK Verbrugh HA van Belkum A 《Journal of microbiological methods》2007,69(2):371-375
We compared multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for typing of Staphylococcus aureus and show that the methods yield similar results, although with differences in resolving power and reproducibility. Epidemiological conditions should determine which is the optimal typing method to be used. 相似文献
8.
Westenbroek C Snijders TA den Boer JA Gerrits M Fokkema DS Ter Horst GJ 《Hormones and behavior》2005,47(5):620-628
Social support has a positive influence on the course of a depression and social housing of rats could provide an animal model for studying the neurobiological mechanisms of social support. Male and female rats were subjected to chronic footshock stress for 3 weeks and pair-housing of rats was used to mimic social support. Rats were isolated or housed with a partner of the opposite sex. A plastic tube was placed in each cage and subsequently used as a 'safe' area in an open field test. Time spent in the tube was used as a measurement of anxiety levels. Chronic stress increased adrenal weights in all groups, except for isolated females who showed adrenal hypertrophy in control conditions. In isolated males, chronic stress resulted in an increase in the time the animals spent in the tube. While stress did not affect this parameter in socially housed males, males with a stressed partner showed a similar response as isolated stressed males. Even though adrenal weights showed that isolated females were more affected by stress, after chronic stress exposure, they spent less time in the tube than socially housed females. Socially housed stressed females spent less time in the 'safe' tube compared to control counterparts, indicating that stress has a gender-specific behavioral effect. In conclusion: pair-housing had a stress-reducing effect on behavior in males. Isolation of females was stressful by itself. Pair housing of females was not able to prevent stress-induced behavioral changes completely, but appeared to reduce the effects of chronic stress. 相似文献
9.
Samaiya A Deo SS Thulkar S Hazarika S Kumar S Parida DK Shukla NK 《World journal of surgical oncology》2005,3(1):3-7
BACKGROUND: Malignant small bowel tumors are very rare and leiomyosarcoma accounts for less than 15% of the cases. Management of these tumors is challenging in view of nonspecific symptoms, unusual presentation and high incidence of metastasis. In this case report, an unusual presentation of jejunal sarcoma and management of liver metastasis with radiofrequency ablation (RFA) is discussed. CASE PRESENTATION: A 45-year-old male presented with anemia and features of small bowel obstruction. Operative findings revealed a mass lesion in jejunum with intussusception of proximal loop. Resection of bowel mass was performed. Histopathological findings were suggestive of leiomyosarcoma. After 3-years of follow-up, the patient developed recurrence in infracolic omentum and a liver metastasis. The omental mass was resected and liver lesion was managed with radiofrequency ablation. CONCLUSION: Jejunal leiomyosarcoma is a rare variety of malignant small bowel tumor and a clinical presentation with intussusception is unusual. We suggest that an aggressive management approach using a combination of surgery and a newer technique like RFA can be attempted in patients with limited metastatic spread to liver to prolong the long-term survival in a subset of patients. 相似文献
10.
High-resolution mapping of genotype-phenotype relationships in cri du chat syndrome using array comparative genomic hybridization 总被引:6,自引:0,他引:6 下载免费PDF全文
Zhang X Snijders A Segraves R Zhang X Niebuhr A Albertson D Yang H Gray J Niebuhr E Bolund L Pinkel D 《American journal of human genetics》2005,76(2):312-326
We have used array comparative genomic hybridization to map DNA copy-number changes in 94 patients with cri du chat syndrome who had been carefully evaluated for the presence of the characteristic cry, speech delay, facial dysmorphology, and level of mental retardation (MR). Most subjects had simple deletions involving 5p (67 terminal and 12 interstitial). Genotype-phenotype correlations localized the region associated with the cry to 1.5 Mb in distal 5p15.31, between bacterial artificial chromosomes (BACs) containing markers D5S2054 and D5S676; speech delay to 3.2 Mb in 5p15.32-15.33, between BACs containing D5S417 and D5S635; and the region associated with facial dysmorphology to 2.4 Mb in 5p15.2-15.31, between BACs containing D5S208 and D5S2887. These results overlap and refine those reported in previous publications. MR depended approximately on the 5p deletion size and location, but there were many cases in which the retardation was disproportionately severe, given the 5p deletion. All 15 of these cases, approximately two-thirds of the severely retarded patients, were found to have copy-number aberrations in addition to the 5p deletion. Restriction of consideration to patients with only 5p deletions clarified the effect of such deletions and suggested the presence of three regions, MRI-III, with differing effect on retardation. Deletions including MRI, a 1.2-Mb region overlapping the previously defined cri du chat critical region but not including MRII and MRIII, produced a moderate level of retardation. Deletions restricted to MRII, located just proximal to MRI, produced a milder level of retardation, whereas deletions restricted to the still-more proximal MRIII produced no discernible phenotype. However, MR increased as deletions that included MRI extended progressively into MRII and MRIII, and MR became profound when all three regions were deleted. 相似文献