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大亚湾海域锥状斯氏藻赤潮及其与孢囊的关系 总被引:22,自引:0,他引:22
用沉积物捕捉器取样,研究了大亚湾海域2000年6~9月孢囊(Cyst)种群动态,同时 分析了浮游植物种群动态。研究期间(8~9月)该海域发生了一起大规模的锥状斯氏藻赤潮。 赤潮前一个月,锥状斯氏藻孢囊的平均日形成率为2.05× 105cysts/(m2·d),丰度为50.22%, 该时期以有性生殖产生的静止期孢囊为主;在赤潮盛期的8,9月初,孢囊的平均日形成率达到 2.01 ×106cysts/(m2·d),丰度为96.97%,此时期的孢囊多为营养细胞所形成的暂时性孢囊; 赤潮结束后,由于环境条件的恶化,暂时性孢囊的形成达到高峰,平均日形成率为1.24×l07 cysts/( m2·d),丰度为99.53%。结果表明大亚湾丰富的孢囊是潜在的种源(Seed bed),2000年 的夏季、秋李持续高温(>25℃),台风减少及风力减弱等有利锥状斯氏藻的环境条件诱发孢 囊萌发,开始了该种类的赤潮。 相似文献
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东海原甲藻的分子鉴定 总被引:4,自引:2,他引:4
采用nrDNAITS序列及18S序列两种分子标记,对东海原甲藻和美国国家海洋藻种保藏中心(CCMP)的具齿原甲藻进行分子鉴定,结果表明,两者之间序列非常相似,两者的nrDNAITS序列碱基差异值仅为0.002,nrDNA18S序列的碱基差异值为0.000,根据分子数据,东海原甲藻和美国国家海洋藻种保藏中心的具齿原甲藻应为同一个种.从基因库获取原甲藻的另外3个种nrD-NAITS序列和8个种的18S序列,用计算机软件进行分析和构建分子系统树,结果显示18S序列用于原甲藻的分子鉴定过于保守,而nrDNAITS更适合于该种属界定的分子标准. 相似文献
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对引发赤潮的3株硅藻——1株尖刺拟菱形藻(Pseudo-nitzshia pungens)和2株中肋骨条藻(Skeletonema costatum)的5.8SrDNA和ITS(internal transcribed spacers)序列进行了PCR扩增、克隆和序列测定,并分析了硅藻门10株赤潮藻(7株从GenBank获得)的系统进化关系.研究结果表明,尖刺拟菱形藻的ITS和5.8SrDNA的长度为693bp,SK-1(分离自东海赤潮暴发区)测序得到715bp,除ITS和5.8SrDNA外,还包含部分18SrDNA和28SrDNA;SK-2(分离自青岛养殖场)的ITS和5.8SrDNA的长度为331bp,尖刺拟菱形藻与从GenBank中获得的2株尖刺拟菱形藻相似程度最高,为100%,与该属的多列拟菱形藻相似程度稍低,为82.9%.SK-2的ITS序列与SK-1的相似程度很低,只有51%,但与拟中肋骨条藻的ITS序列相似程度高,为95.5%.SK-1的ITS序列与拟中肋骨条藻的相似程度也低,为56.7%.系统进化树反映的结果与相似性反映的结果一致.研究的该株尖刺拟菱形藻从根据ITS序列研究的结果与形态鉴定的结果看是一致的;SK-2可能属于拟中肋骨条藻;SK-1比较特殊,有待于用其他的方法进一步研究确定其分类地位. 相似文献
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Phaeocystis globosa Scherffel, a harmful microalga, and its production of dimethylsulfoniopropionate
The production of dimethylsulfoniopropionate (DMSP) and its cleavage products (DMS) are well studied in phytoplankton worldwide. However, less is known about their sources, distributions, and impacts in the coast of China. We examined the production of DMSP and DMS in Phaeocystis globosa Scherffel and other benthic macroalgae from the South China coast in relation to environmental conditions. P. globosa was a harmful marine microalgal species and its bloom took place in the eutrophic waters along the South China Sea frequently. It also produced high content of DMSP at different growth stages, with the highest concentration usually observed in the stationary period. Moreover, the production of DMSP in P. globosa was significantly affected by salinity and temperature with the highest contents associated with high salinity (e.g. 40) and low temperature (e.g. 20°C). In field benthic macroalgae, there was also a marked difference in the DMSP of various species or different samples of the same species. Chlorophyll a contents were also determined for each macroalgal species. The highest chlorophyll a (238.7 ng/g fresh weight) was recorded in Chlorophyta Ulva lactuca at Guishan Island (Zhuhai), while the lowest value (1.5 ng/g fresh weight) was found in Rhodophyta Gracilaria tenuistipitata in Zhanjiang. Further correlation analysis indicated that there was no significant relationship between the content of DMSP and chl-a in macroalgae samples (P > 0.05). All the results suggested that the production of DMSP in marine algae was not only species- and stage-related, but also greatly affected by various environmental factors. 相似文献
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