痰热清注射液与5种输液配伍后的澄明度及不溶性微粒考察

目的对痰热清注射液在5种输液中的澄明度及不溶性微粒进行考察,探求该药不良反应发生原因,为临床合理用药提供参考。方法模拟临床实际配伍操作,将混合液注入试管内按一定的时间间隔进行澄明度观察,同时依据显微计数法对不溶性微粒进行测定。结果除0.9%氯化钠注射液外,痰热清与4种输液的配伍液随放置时间的延长均可析出较多微粒。其中,10%葡萄糖及果糖配伍液中微粒数最多,放置180min后澄明度可发生变化。结论痰热清的最佳溶媒是0.9%氯化钠注射液,用5%葡萄糖或木糖醇注射液应现配现用,勿放置时间过长,避免不良反应发生。     

抗栓肽Ala~(34)→Trp突变体的表达及活性研究

目的:将抗栓肽34位的丙氨酸突变为色氨酸,构建活性较高的突变体。方法:通过设计合成两对引物经PCR获得抗栓肽突变体基因,利用质粒pET32a( )作为载体,在大肠杆菌BL21(DE4)内表达。利用融合蛋白本身的组氨酸标签,以金属亲和色谱进行纯化,然后用肠激酶切除融合部分,所得蛋白用体外抗血小板凝集实验检测其活性。结果与结论:抗栓肽突变体以可溶形式高效表达,活性检测显示其抑制二磷酸腺苷诱导的血小板聚集的IC50为150 nmol/L。与原型蛋白相比,突变体活性明显提高。     

男性不育症患者解脲支原体感染与精液质量的关系

1973年Gnarpe从一组不明原因的不育症患者精液中分离出了解脲支原体(Ureaplasma Urealyticum,UU),提出UU的生殖道感染可能与男性不育有关[1],受到国内外学者关注.为了探讨UU感染与精液异常的关系,为临床诊断提供有价值的信息,本研究对315例不育症患者UU感染情况及精液质量进行检测分析,现报告如下.     

加温合并放射治疗恶性肿瘤的实验研究 Ⅰ.微波加温治疗大鼠移植Walker癌肉瘤256的实验研究

本文报告用微波(2450MHz)一次加温(44℃,35～40分钟)治疗大鼠移植Walker癌肉瘤256的结果。实验证明,加温组动物生存期较对照组长。加温组27只大鼠中有7只肿瘤完全消退。观察1 1/2～10个月,未见复发。 形态学变化,组织化学及pH值观察表明,加温后癌组织的变性坏死是一个渐进的、复杂的代谢和结构变化过程。加温后早期可见瘤组织内血管壁的Mg~( )-A-TP酶活性明显降低,pH值显著下降,初级溶酶体增多,线粒体肿胀,嵴断裂或消失,内质网扩张;随若时间延长,出现次级溶酶体增多、核碎裂。电镜和光镜均证实加温对癌细胞有杀伤作用。瘤体坏死以中心为重。     

放射治疗学新进展(续)

(三)综合疗法的成绩及展望: 放射治疗与手术,化疗与放射,手术、化疗、放疗三者的综合治疗都已有成功的经验及突出的新进展。现简要介绍与放射有关的常用综合疗法: 1.术前放射:近年倾向于术前放射。理论研究及动物实验均证实放射可缩小原发瘤,使手术便于进行;消灭亚临床灶,减少手术播散或种植,从而降低局部复发率并提高疗效,     

BLADDER CANCER AND RADIATION DAMAGE

When one has difficulty in distinguishing bladder cancer from radiation damage, close cooperation among specialists and follow up are of great im portance for a correct diagnosis and appropriate treatment. Previous irradiation and infection of pelvic organs, the bladder in particular, are two of the important factors in producing radiation damage. The causes of misdiagnosis vary. If both cystoscopic and histologic appearances are very confusing, close follow up including careful cystoscopic observation and repeated biopsy is recommended other than a hasty diagnosis of cancer followed by intensive therapy.     

放射治疗学名词汇编(下)

Radiocurability 放射治愈性用电离辐射手段治愈某和疾病的能力。与放射敏感性不是同意语,因为很多肿瘤虽是放射敏感的但也常有转移,中等敏感性肿瘤的治愈率反而相对地高。Radiology 放射学用电离辐射诊断与治疗疾病的一门医学专业。近代,其分支包括:放射诊断学、放射治疗学、核医学、放射生物学、医用放射物理学和放射防护学。     

固醇调节元件结合蛋白-1c基因54G/C多态性对高糖低脂膳食诱导的健康青年人血脂比值的影响

Objective To investigate the effects of 54G/C polymorphism of sterol regulatory elementbinding protein-1c gene (SREBP-1c)on serum lipid ratios and their response to high-carbohydrate/low-fat(HC/LF) diet in healthy youth. Methods After a regular diet for 7 days of wash-out, 56 healthy youth (22.89±1.80 yrs) were given HC/LF diet for 6 days. The regular diet contained 54% carbohydrate, 15%protein, and 31% fat of the total energy. The HC/LF diet contained 70% carbohydrate, 15% protein, and 15% fat of the total energy. The serum lipids and glucose were measured on the 1st, 8th and 14th days.The ratios of TG/HDL-C, log (TG/HDL-C), TC/HDL-C, and LDL-C/HDL-C were calculated. The 54G/C polymorphism of SREBP-1c gene was analyzed by PCR-RFLP method. Results No significant difference was found in lipid ratios and glucose at baseline and after regular diet in subjects with different genotypes in either the whole studied population or in males or females only. However, after HC/LF diet, LDL-C/HDL-C was significantly lower in females carrying the C allele than those of GG homozygotes (P＜ 0.05).Compared with those before HC/LF diet, TC/HDL-C and LDL-C/HDL-C were significantly decreased in all the subjects (P＜0.05). When gender was taken into account, significant increase of TG/HDL-C and log(TG/HDL-C) was found only in females with GG genotype (P＜0.05). All the subjects experienced significant decrease of TC/HDL-C and LDL-C/HDL-C regardless of their genders and genotypes (P＜0.05). Conclusion The 54G/C polymorphism of SREBP-1c gene can influence the response of TG/HDL-C and log(TG/HDL-C) to HC/LF diet in females. The C allele may be a protective factor to prevent the increase of TG induced by HC/LF diet in females.     

脑得生咀嚼片微生物限度检查方法学验证

目的:建立脑得生咀嚼片微生物限度检查方法。方法:按中国药典方法进行试验,以回收率对方法有效性进行评价。结果:采用常规法,5种试验菌的回收率均>70%。结论:脑得生咀嚼片无抑菌活性,可用常规法进行微生物限度检查,可行性强,能达到检测目的。