胰岛细胞瘤的外科诊治分析(附16例报告)

目的探讨胰岛细胞瘤的诊断和治疗方法。方法对我院2000年6月～2006年12月收治的胰岛细胞瘤16例的临床资料进行回顾性分析。结果本组16例经手术治疗,功能性胰岛细胞瘤13例术后血糖均恢复正常,非功能性细胞瘤3例术后腹部症状消失。术后发生并发症3例,经治疗后痊愈出院。结论术前、术中影像学检查可以提高手术切除率。手术切除胰岛细胞瘤可以获得满意效果。     

ERCP联合LC治疗胆囊结石合并胆总管结石的临床分析

目的探讨经内镜逆行胰胆管造影术(ERCP)、内镜十二指肠乳头括约肌切开术(EST)联合腹腔镜胆囊切除术(LC)治疗胆囊结石合并胆总管结石的临床疗效。方法回顾性分析采用ERCP/EST联合LC方法治疗的40例胆囊结石合并胆总管结石的患者的临床资料。结果 ERCP/EST手术成功率97.5%(39/40),1例插管失败,LC手术成功率97.4%(38/39),1例中转开腹手术,8例(20.5%)患者ERCP/EST术后出现高淀粉酶血症,无出血、胆瘘、十二指肠瘘、胆管损伤及胆道结石残留等并发症发生。61.5%(24/39)患者ERCP/EST术后2~5天行LC术,38.5%(15/39)患者ERCP/EST术后6~14天行LC术,前者在住院时间及住院费用上明显低于后者。结论 ERCP/EST联合LC治疗胆囊结石合并胆总管结石安全可行,具有患者创伤小、康复快、并发症少等优点,ERCP术后尽早施行LC可减少患者住院天数及住院费用。     

外周血中CD4~-CD8~-T细胞的分离及生物学特性检测

目的分离和培养外周血中CD4-CD8-T细胞,检测其生物学活性。方法应用免疫磁珠法分离CD4-CD8-T细胞,流式细胞仪检测CD4-CD8-T细胞纯度,ELISA法检测CD4-CD8-T细胞分泌的IL-4、IFN-γ含量。结果CD4-CD8-T细胞分离后纯度为82.77%,CD4-CD8-T细胞分泌IFN-γ的量为(159.28±38.16)pg/ml,而IL-4含量未能检测出。结论CD4-CD8-T细胞可分泌IFN-γ,基本不分泌IL-4。     

嵌顿性腹股沟斜疝一期无张力疝修补27例患者的疗效

目的 探讨一期无张力疝修补术治疗嵌顿性腹股沟斜疝的临床效果.方法 回顾性分析合肥市第三人民医院2010年1月至2016年12月收治的27例成人无肠坏死或肠穿孔的嵌顿性腹股沟斜疝一期无张力疝修补术27例患者临床资料.结果 27例患者均治愈出院,平均手术时间(81.9±23.3)min,平均术中出血量(16.5±22.8)mL,平均住院时间(7.7±2.3)d;25例患者切口甲级愈合,1例切口有渗液及1例切口局部淤血患者均对症处理后痊愈,术后随访6个月以上,均无复发.结论 如无肠坏死或肠穿孔,一期无张力疝修补术治疗嵌顿性腹股沟斜疝效果良好.     

The relationship between CD4-CD8-T cells in the peripheral blood of patients with pancreatic carcinoma and IL-4,IFN-γ levels

Objective To investigate the relationship between CD4-CD8-T cells ratio and IL-4, IFN-γ levels in the peripheral blood of patients with pancreatic carcinoma. Methods Floweytometer was used to analyze the CD4-CD8-T cells ratio in the peripheral blood of patients with pancreatic carcinoma and the IL-4 ,IFN-γ levels were detected by ELISA. Results The ratio of CD4-CD8-T cell in CD3+ T cell from 25 pancreatic carcinoma patients was(4.2 ± 1.7) %, the ratio of CD4-CD8-T cell in CD3+T cell from 45 healthy person was(6.3 ± 2.6) %, there was significant deviation between the two groups(P ＜ 0. 01). The IL-4 level of 25 pancreatic carcinoma patients was (86.3 ± 23.3) fg/L,the IL-4 level of 45 healthy person was (56.2 ± 9.2) fg/L,there was significant deviation between the two groups(P ＜0.01). The IFN-γ level of 25 pancreatic carcinoma patients was (16.4±4.8) fg/L before operation, the IFN-γ level of 45 healthy person was (27.4±3.8) fg/L,there was significant deviation between the two groups(P ＜0.01). The ratio of CD4-CD8-T cell in pancreatic carcinoma patient after operation was higher than before operation. It could be found negative correlation between CD4-CD8-T cells ratio and IL-4 level in pancreatic carcinoma patient,it could also be found positive correlation between CD4-CD8-T cells ratio and IFN-γ level in pancreatic carcinoma patient. In pancreatic carcinoma patient,the CD4-CD8-T cells ratio and IL-4 level was significant associated with clinical stage (P ＜ 0.05), but no relationship with hisological differentiation (P＞0.05), it could be found no relationship between IFN-γ level and clinical stage, hisoiogieal differentiation (P ＞ 0.05). Conclusion The CD4±CD8±T cells ratio in the peripheral blood of patients is decreased,it may be participate in the carcinogenesis and development of pancreatic carcinoma by influence the IFN-γ levels.     

腹股沟疝合并精索脂肪瘤诊治分析

目的 总结腹股沟疝合并精索脂肪瘤的诊断及手术治疗经验,为提高临床对该病的诊治水平提供参考。方法 回顾分析2016年1月至2021年7月合肥市第三人民医院收住院的116例接受开放性腹股沟疝修补术的腹股沟疝患者临床资料,分析术中发现的24例腹股沟疝合并精索脂肪瘤的诊断及手术技巧。结果 本组腹股沟疝合并精索脂肪瘤的发生率为20.7%(24/116),24例腹股沟疝合并精索脂肪瘤患者均在开放性疝修补术中分离至内环口以上水平后切除,术后无复发。24例患者腹股沟疝Nyhus分型,21例为Ⅱ型、Ⅲ型,占87.5%。精素脂肪瘤病理分型:血管脂肪瘤1例,纤维脂肪瘤6例,脂肪瘤17例。结论 腹股沟疝合并精索脂肪瘤有较高的发生率,通常在术中确诊,应予术中游离至内环口以上水平切除。     

DNT细胞分离鉴定及其在正常人外周血中含量的检测

目的 建立人DNT细胞的免疫磁性分离(megnetic activated cell sorting,MACS)方法,并检测其在正常人外周血中含量.方法 运用MACS法分离止常人外周血中DNT细胞,用台盼蓝染色流式细胞法检测选出细胞的活性和纯度,运用流式细胞法检测其在正常人外周血中含量.结果 MACS分选法分选出的DNT细胞活性＞97%,纯度为82.77%;DNT细胞占TCRαβ+T细胞的比例(6.25±2.61)%(n=45).结论 MACS法可简单迅速分选出高纯度DNT细胞,且不影响细胞活力.DNT细胞占TCRCD3+T细胞的比例为(6.25±2.61)%.     

The relationship between CD4-CD8-T cells in the peripheral blood of patients with pancreatic carcinoma and IL-4,IFN-γ levels

Objective To investigate the relationship between CD4-CD8-T cells ratio and IL-4, IFN-γ levels in the peripheral blood of patients with pancreatic carcinoma. Methods Floweytometer was used to analyze the CD4-CD8-T cells ratio in the peripheral blood of patients with pancreatic carcinoma and the IL-4 ,IFN-γ levels were detected by ELISA. Results The ratio of CD4-CD8-T cell in CD3+ T cell from 25 pancreatic carcinoma patients was(4.2 ± 1.7) %, the ratio of CD4-CD8-T cell in CD3+T cell from 45 healthy person was(6.3 ± 2.6) %, there was significant deviation between the two groups(P ＜ 0. 01). The IL-4 level of 25 pancreatic carcinoma patients was (86.3 ± 23.3) fg/L,the IL-4 level of 45 healthy person was (56.2 ± 9.2) fg/L,there was significant deviation between the two groups(P ＜0.01). The IFN-γ level of 25 pancreatic carcinoma patients was (16.4±4.8) fg/L before operation, the IFN-γ level of 45 healthy person was (27.4±3.8) fg/L,there was significant deviation between the two groups(P ＜0.01). The ratio of CD4-CD8-T cell in pancreatic carcinoma patient after operation was higher than before operation. It could be found negative correlation between CD4-CD8-T cells ratio and IL-4 level in pancreatic carcinoma patient,it could also be found positive correlation between CD4-CD8-T cells ratio and IFN-γ level in pancreatic carcinoma patient. In pancreatic carcinoma patient,the CD4-CD8-T cells ratio and IL-4 level was significant associated with clinical stage (P ＜ 0.05), but no relationship with hisological differentiation (P＞0.05), it could be found no relationship between IFN-γ level and clinical stage, hisoiogieal differentiation (P ＞ 0.05). Conclusion The CD4±CD8±T cells ratio in the peripheral blood of patients is decreased,it may be participate in the carcinogenesis and development of pancreatic carcinoma by influence the IFN-γ levels.     

类肝素酶在胰腺癌组织中的表达意义

目的 探讨胰腺癌组织中类肝素酶(heparanase 1,Hpal)表达与胰腺癌侵袭性、临床病理等因素之间的关系.方法 采用逆转录-聚合酶链反应(RT-PCR)、免疫组化(SP)法检测37例胰腺癌患者痛组织、癌旁组织及正常胰腺组织中Hpal表达,并统计分析Hpal表达与胰腺癌侵袭性、临床病理等因素之间的相关性.结果 Hpal在胰腺癌组织、癌旁组织、正常胰腺组织中的表达阳性率分别为64.9%(24/37)、56.8%(21/37)和10.8%(4/37).胰腺癌组织中Hpal表达与胰腺癌TNM分期、侵及神经和血管情况、包膜完整情况以及淋巴转移明显相关(P＜0.05),但与患者年龄、性别、组织学分化程度、肿瘤大小无关(P＞0.05).结论 Hpal与胰腺癌发生、发展密切相关,Hpal表达与胰腺癌的侵袭性、局部浸润、临床分期以及区域淋巴结转移有密切关系,胰腺癌Hpal过表达可促进胰腺癌浸润生长与转移,其表达对评价胰腺癌预后亦有一定的价值.     

The relationship between CD4-CD8-T cells in the peripheral blood of patients with pancreatic carcinoma and IL-4,IFN-γ levels

Objective To investigate the relationship between CD4-CD8-T cells ratio and IL-4, IFN-γ levels in the peripheral blood of patients with pancreatic carcinoma. Methods Floweytometer was used to analyze the CD4-CD8-T cells ratio in the peripheral blood of patients with pancreatic carcinoma and the IL-4 ,IFN-γ levels were detected by ELISA. Results The ratio of CD4-CD8-T cell in CD3+ T cell from 25 pancreatic carcinoma patients was(4.2 ± 1.7) %, the ratio of CD4-CD8-T cell in CD3+T cell from 45 healthy person was(6.3 ± 2.6) %, there was significant deviation between the two groups(P ＜ 0. 01). The IL-4 level of 25 pancreatic carcinoma patients was (86.3 ± 23.3) fg/L,the IL-4 level of 45 healthy person was (56.2 ± 9.2) fg/L,there was significant deviation between the two groups(P ＜0.01). The IFN-γ level of 25 pancreatic carcinoma patients was (16.4±4.8) fg/L before operation, the IFN-γ level of 45 healthy person was (27.4±3.8) fg/L,there was significant deviation between the two groups(P ＜0.01). The ratio of CD4-CD8-T cell in pancreatic carcinoma patient after operation was higher than before operation. It could be found negative correlation between CD4-CD8-T cells ratio and IL-4 level in pancreatic carcinoma patient,it could also be found positive correlation between CD4-CD8-T cells ratio and IFN-γ level in pancreatic carcinoma patient. In pancreatic carcinoma patient,the CD4-CD8-T cells ratio and IL-4 level was significant associated with clinical stage (P ＜ 0.05), but no relationship with hisological differentiation (P＞0.05), it could be found no relationship between IFN-γ level and clinical stage, hisoiogieal differentiation (P ＞ 0.05). Conclusion The CD4±CD8±T cells ratio in the peripheral blood of patients is decreased,it may be participate in the carcinogenesis and development of pancreatic carcinoma by influence the IFN-γ levels.