Properties of poly(ethylene terephthalate)–poly(ethylene naphthalene 2,6‐dicarboxylate) blends with montmorillonite clay

The production and properties of blends of poly(ethylene terephthalate) (PET) and poly(ethylene naphthalene 2,6‐dicarboxylate) (PEN) with three modified clays are reported. Octadecylammonium chloride and maleic anhydride (MAH) are used to modify the surface of the montmorillonite–Na⁺ clay particles (clay–Na⁺) to produce clay–C18 and clay–MAH, respectively, before they are mixed with the PET/PEN system. The transesterification degree, hydrophobicity and the effect of the clays on the mechanical, rheological and thermal properties are analysed. The PET–PEN/clay–C18 system does not show any improvements in the mechanical properties, which is attributed to poor exfoliation. On the other hand, in the PET–PEN/clay–MAH blends, the modified clay restricts crystallization of the matrix, as evidenced in the low value of the crystallization enthalpy. The process‐induced PET–PEN transesterification reaction is affected by the clay particles. Clay–C18 induces the largest proportion of naphthalate–ethylene–terephthalate (NET) blocks, as opposed to clay–Na⁺ which renders the lowest proportion. The clay readily incorporates in the bulk polymer, but receding contact‐angle measurements reveal a small influence of the particles on the surface properties of the sample. The clay–Na⁺ blend shows a predominant solid‐like behaviour, as evidenced by the magnitude of the storage modulus in the low‐frequency range, which reflects a high entanglement density and a substantial degree of polymer–particle interactions. Copyright © 2005 Society of Chemical Industry     

A role for Edman degradation in proteome studies

Advances in protein database design and the software used to access the sequence data has led to progress in using protein attributes such as amino acid composition and peptide masses to identify proteins separated by two-dimensional electrophoresis. However, Edman degradation remains the principal technique for protein identification and it presents a significant bottleneck in the progress towards rapid protein identification. Simple modifications to the sequencing hardware, which automate the delivery of protein spots into the sequencer, and parallel sequencing of the protein spots represent a significant advance in the use of Edman degradation to rapidly generate the powerful protein attribute, an N-terminal sequence tag.     

Protein expression profiles in human breast ductal carcinoma and histologically normal tissue

Reference two-dimensional (2-D) gels are presented for human breast ductal carcinoma and histologically normal tissue. Whole biopsy fragments were analyzed, including epithelial and nonepithelial components. Thirty-five spots have been assigned by gel matching to the human liver SWISS-2DPAGE reference map and/or to the human primary keratinocyte IPG map from the Danish Center for Human Genome. N-terminal microsequencing was applied to confirm randomly chosen matching assignments and to identify six new spots. Protein expression profiles in ductal carcinoma and in normal breast tissue appeared to be similar, except for a pattern consisting of 32 spots, which were highly expressed in all carcinoma specimens, and less intense and occasionally undetectable in normal tissue. This difference was statistically significant. Assignment has been obtained for several spots, namely GRP94, GRP78, GRP75, mitochondrial HSP60, calreticulin, protein disulfide isomerase, peptidyl-prolyl cis-trans isomerase, collagen-binding protein 2, fructose bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, thioredoxin, cytochrome c oxidase VA subunit, tubulin beta isoform and macrophage migration inhibitory factor (MIF). The cancer- and tissue-specificity of the described pattern was assessed by matching to the Swiss-2DPAGE human liver, hepatoma, lymphoma, erythroleukemia reference maps. The pattern of 32 spots was found to be indicative of epithelial neoplasia.     

New actinide ternary intermetallic compounds: synthesis, characterization and physical properties

Twelve new ternary intermetallic compounds of light actinide elements from Th to Pu, namely U₄Tc₇Si₆, U₄Tc₇Ge₆, Np₄Ru₇Ge₆, U₂Tc₃Si₅, Np₂Re₃Si₅, Pu₂Re₃Si₅, Th₂Mo₃Si₄, U₂Mo₃Si₄, U₂Mo₃Ge₄, Np₂Mo₃Si₄, Np₂Mo₃Ge₄, and Pu₂Mo₃Si₄, were obtained. The structures of these compounds are discussed as well as some preliminary physical property studies. Magnetic susceptibility measurements show that An₂Mo₃(Si or Ge)₄ compounds are paramagnetic at all temperatures studied and that U₄Tc₇Si₆ orders antiferromagnetically around 25 K. Mössbauer spectroscopy on Np₄Ru₇Ge₆ does not show a magnetic splitting down to 4.2 K.     

Developing maintainable expert systems using case-based reasoning

Abstract: Maintainability problems associated with traditional software systems are exacerbated in rule-based systems. The very nature of that approach — separation of control knowledge and data-driven execution — hampers maintenance. While there are widely accepted techniques for maintaining conventional software, the same is not true for rule-based systems. In most situations, both a knowledge engineer and a domain expert are necessary to update the rules of a rule-based system. This paper presents, first, an overview of the software engineering techniques and object-oriented methods used in maintaining rule-based systems. It then discusses alternate paradigms for expert system development. The benefits of using case-based reasoning (from the maintenance point of view) are illustrated through the implementation of a case-based scheduler. The main value of the scheduler is that its knowledge base can be modified by the expert without the assistance of a knowledge engineer. Since changes in application requirements can be given directly to the system by the expert, the effort of maintaining the knowledge base is greatly reduced.