Use of Zebrafish Embryo Assay to Evaluate Toxicity and Safety of Bioreactor-Grown Exopolysaccharides and Endopolysaccharides from European Ganoderma applanatum Mycelium for Future Aquaculture Applications

Natural mycelial exopolysaccharide (EPS) and endopolysaccharide (ENS) extracted from bioreactor-cultivated European Ganoderma applanatum mushrooms are of potential high commercial value for both food and adjacent biopharmaceutical industries. In order to evaluate their potential toxicity for aquaculture application, both EPS (0.01–10 mg/mL) and ENS (0.01–10 mg/mL) extracts were tested for Zebrafish Embryo Toxicity (ZFET); early development effects on Zebrafish Embryos (ZE) were also analyzed between 24 and 120 h post-fertilization (HPF). Both EPS and ENS are considered non-toxic with LC₅₀ of 1.41 mg/mL and 0.87 mg/mL respectively. Both EPS and ENS did not delay hatching and teratogenic defect towards ZE with <1.0 mg/mL, respectively. No significant changes in the ZE heart rate were detected following treatment with the two compounds tested (EPS: 0.01–10 mg/mL: 176.44 ± 0.77 beats/min and ENS: 0.01–10 mg/mL: 148.44 ± 17.75 beats/min) compared to normal ZE (120–180 beats/min). These initial findings support future pre-clinical trials in adult fish models with view to safely using EPS and ENS as potential feed supplements for supplements for development of the aquaculture industry.     

A cathode support structure for use in a magnetron oscillator experiment

A low temperature co-fired ceramic (LTCC) material system has been used to develop a protype field emission cathode structure for use in an experimental magnetron oscillator. The structure is designed for used with 30 gated field emission array (GFEA) die electrically connected through silver metal traces and electrical vias. To approximate a cylinder, the cathode structure (48 mm long and 13.7 mm in diameter) is comprised of 10 faceted plates which cover the GFEA dies. Slits in the facet plates allow electron injection. The GFEA die (3 mm × 8 mm) are placed in axial columns of 3 and spaced azimuthally around a cylindrical support structure in a staggered configuration resulting in 10 azimuthal locations. LTCC manufacturing techniques were developed in order to fabricate the newly designed cathode with seven layers wrapped to form the cylinder with electrical traces and vias. Two different cathode wrapping techniques and two different via filling techniques were studied and compared. Two different facet plate manufacturing techniques were studied. Finally, four different support stand configurations for firing the cylindrical structure were also compared with a square post stand having the best circularity and linearity measurements of the fired structure.     

Al2O3/Yttria‐Stabilized Zirconia Hollow‐Fiber Membrane Incorporated with Iron Oxide for Pb(II) Removal

Removal by absorptive ceramic membranes can simultaneously absorb and separate metal ions from water. Alumina/yttria‐stabilized zirconia (Al₂O₃/YSZ) hollow‐fiber membranes, fabricated using phase inversion and sintering process, were deposited with iron oxide by an in‐situ hydrothermal process. The results showed that α‐Fe₂O₃ was produced and incorporated across the membranes. A reduction in flux was recorded with the deposition of α‐Fe₂O_3. However, it improved the adsorption capacity for heavy metal adsorption. The adsorption‐separation test demonstrated that the optimized membrane is able to completely remove Pb(II) ions after two hours.     

Bond graph modeling,design and experimental validation of a photovoltaic/fuel cell/ electrolyzer/battery hybrid power system

This work presents a complete bond graph modeling of a hybrid photovoltaic-fuel cell-electrolyzer-battery system. These are multi-physics models that will take into account the influence of temperature on the electrochemical parameters. A bond graph modeling of the electrical dynamics of each source will be introduced. The bond graph models were developed to highlight the multi-physics aspect describing the interaction between hydraulic, thermal, electrochemical, thermodynamic, and electrical fields. This will involve using the most generic modeling approach possible for managing the energy flows of the system while taking into account the viability of the system. Another point treated in this work is to propose. In this work, a new strategy for the power flow management of the studied system has been proposed. This strategy aims to improve the overall efficiency of the studied system by optimizing the decisions made when starting and stopping the fuel cell and the electrolyzer. It was verified that the simulation results of the proposed system, when compared to simulation results presented in the literature, that the hydrogen demand is increased by an average of 8%. The developed management algorithm allows reducing the fuel cell degradation by 87% and the electrolyzer degradation by 65%. As for the operating time of the electrolyzer, an increment of 65% was achieved, thus improving the quality of the produced hydrogen. The Fuel Cell's running time has been decreased by 59%. With the ambition to validate the models proposed and the associated commands, the development of this study gave rise to the creation of an experimental platform. Using this high-performance experimental platform, experimental tests were carried out and the results obtained are compared with those obtained by simulation under the same metrological conditions.     

Synthesis of heterocyclic metal complexes and their evaluation as marine antifoulants

4-Amino-3-thio-1,24-triazolidine (L₁) and 4-amino-5-thio-1,3,4-thiadiazole (L_II) as well as their metal complexes of the general formulae ML_I·2H₂O and ML_IICl (M; Pb(II), Cd(II) and Zn(II)) were prepared. With Sn(II), we obtained Sn(L_I)₂·2H₂O and SnL_IICl, respectively. The structures of the compounds were identified through elemental analysis, and IR and UV spectra measurements, in addition to thermal analysis in case of the metal complexes. The antifouling properties of the compounds were tested by their incorporation into paint formulations which were applied to PVC substrates and tested in water from Alexandria western harbour. When the compounds were added at 17·5% by volume, the coated panels were fouled after 3 months of immersion. The addition of 6·7% by volume of tributyltin oxide to 15·8% of the prepared compounds in one formulation elevated the paint efficiency and prevented fouling for 11 months. Paint containing solely tributyltin oxide at the same concentration was inactive against algae.     

Dopamine receptors influence vasoactive intestinal peptide release from turkey hypothalamic explants

Amongst the elements which contributed to the success of the early lung transplants at the beginning of the 1980's we feel that the careful selection of candidates probably played a predominant role. If some of the selection criteria initially described remain somewhat intangible, others have either been eased or have been invalidated. The experience acquired over the last 15 years has enabled to precise the optimal moment to include patients on the waiting list and to refine the choice for the type of surgical procedure according to the underlying disease. This article aims to review the different selection criteria for candidates for transplantation and stresses those which have recently undergone change.     

Dispersion analysis of multilayer cylindrical transmission linescontaining magnetized ferrite substrates

Recently, there has been a growing interest in using cylindrical transmission lines that contain magnetized ferrite material in a variety of applications. In this paper, the finite-difference time-domain (FDTD) method (in cylindrical coordinates) and the spectral-domain analysis (SDA) are used to calculate the propagation characteristics of cylindrical transmission lines that contain magnetized ferrite material. The magnetization can be either in the longitudinal or azimuthal directions. Specifically, the cylindrical microstrip line, and the cylindrical coplanar waveguide printed on magnetized ferrite substrate are analyzed. Both the FDTD and SDA results are in very good agreement. In addition, the results are compared to those of planar structures by taking the radius of the substrate to be large enough such that the curvature effect is negligible