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In automated mechanical transmissions, engaging sleeve with clutch gear causes the switches of mechanical coupling modes of engaging-related parts, and impacts may happen to make the part speeds have discrete transitions during the engaging. The two characters make the engaging process exhibit both continuity and discreteness. The aim of the paper is to develop a high-fidelity model to capture the trajectories of the parts in the transmission. We treat the engaging as a two-phase process—sleeve first interacting with synchro ring and then with clutch gear. The part movements under a certain constraint are governed by multibody dynamics, and the speed jumps caused by impacts are described using the Poisson coefficient of restitution. To couple the continuous evolutions and discrete transitions of the states, a hybrid automaton model is developed. This model allows for the characteristic analysis of the engaging process with multiple interaction cases between the engaging-related parts, and it is validated by bench tests. The effects of two key factors—the relative tooth position of sleeve and clutch gear and the shifting force—on engaging duration and impact are studied through numerical simulations.  相似文献   
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Nowadays islanded microgrids mostly rely on diesel generator. In order to reduce greenhouse emissions, two islanded microgrids with hydrogen storage have been installed and are currently working autonomously in Reunion Island and France. Energy management implemented on these stations fall in the myopic control category. This study aims to determine the performance improvement that could be achieve on such stations using model predictive control. Ability to supply to the loads and energy losses minimisation are our main objectives. Lifetime degradation is also taken in consideration. Simulations shows that significant improvement can be brought, with a 76% decrease of the station's defaults time and better fill rate of hydrogen tank and batteries. In the meantime, chemical and electrical losses are reduced by 38 and 11%, and the batteries degradations are decreased by around 1%. The relevance of taking into account the electrolyser and fuel cell degradations depends on the time-step of the control. These results give a performance target in order to implement a real-time model predictive control in the microgrids, and eventually can be used to better sizing of future microgrids with similar architecture.  相似文献   
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The majority of studies dealing with DNA analyses are made on fixed cells. In this context, the efficiency as fixatives of ethanol, methanol, acetone, Carnoy, Boehm-Sprenger and aldehydes was determined using two different DNA fluorescent probes, Hoechst 33342 and propidium iodide. The purpose of our study was to find the fixative that would provide the best results with respect to the following parameters: aggregates, cell size and granularity, and DNA staining analysis. Using murine fibroblasts, we found that 68% ethanol, 85% methanol and aldehydes did not increase aggregate formation, whereas Carnoy, acetone or Boehm-Sprenger fixatives did. The results show that aldehydes seem to alter cell size least. All fixatives induce an increase in cell granularity, which is very pronounced with alcohols, but aldehydes alter morphology less than alcohols. We observed that the fixatives giving the best resolution with Hoechst 33342 staining lead to a lower measurement variability than with propidium iodide staining. This study leads us to conclude that 68% ethanol and 85% methanol can be considered as appropriate fixatives for flow cytometry studies of DNA content.  相似文献   
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In this paper a discrete-time dynamic random graph process is studied that interleaves the birth of nodes and edges with the death of nodes. In this model, at each time step either a new node is added or an existing node is deleted. A node is added with probability p together with an edge incident on it. The node at the other end of this new edge is chosen based on a linear preferential attachment rule. A node (and all the edges incident on it) is deleted with probability q=1−p. The node to be deleted is chosen based on a probability distribution that favors small-degree nodes, in view of recent empirical findings. We analyze the degree distribution of this model and find that the expected fraction of nodes with degree k in the graph generated by this process decreases asymptotically as k−1−(2p/2p−1).  相似文献   
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In this paper we describe the development of the first multiclass pesticide residue method applied to essential oils. A total of 70 pesticides covering a wide range of polarity and currently used on essential oil crops have been included in the method. The procedure consists of a 10-fold dilution of lavandin essential oil followed by a direct injection analysis by liquid chromatography/tandem mass spectrometry. The system used is an API 4000 QTrap equipped with an electrospray ionization interface and operating in scheduled selected reaction monitoring acquisition mode. Matrix effects were evaluated by comparing the slopes of matrix-matched and solvent-based calibration curves. Weak signal suppression or enhancement (<20%) was observed for most of the compounds. Method sensitivity was determined statistically by the injection of five matrix-matched calibration curves with the distribution's normality and the variance's homogeneity checked before establishment of a suitable regression model. Limits of detection (LODs) and quantification (LOQs) were then determined using the blank standard's deviation and the slope of the mean curve. The analytical method has been validated for 67 of the 70 pesticides and meets the following LOQs: ≤1 μg/L for 9 pesticides, ≤5 μg/L for 44, ≤10 μg/L for 9, and ≤20 μg/L for 5.  相似文献   
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CsNiCr(CN)6 coordination nanoparticles with sizes ranging from 6 to 30 nm are highly diluted in an organic polymer matrix. Their static and dynamic magnetic behaviour allows unravelling of surface anisotropy and interparticle dipolar interaction effects. The single magnetic domain critical size is thus evaluated to be around 22 nm with a blocking temperature of 21 K (at ν = 1 Hz) and an effective energy barrier for the reversal of the magnetization of 426 K.  相似文献   
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Magnetic nanocrystals organized in chain-like structures behave as nanowires with a shape anisotropy induced by the structuration of the sample. This is valid for various types of magnetic nanomaterials. Furthermore, the coating of nanocrystals plays a major role in the mesoscopic structure of the film. γ-Fe2O3 nanocrystals are used as a mask to reproduce the mesoscopic structure on a silicon wafer.  相似文献   
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