Investigations on tyrosinase activity in melanin-free ink from Sepia officinalis: potential for food proteins cross-linking

Protein modification via enzymatic cross-linking is an attractive way for altering food structure so as to create products with increased quality and nutritional value. In this study, enzymatic cross-linking of β-casein was performed by tyrosinase activity, from melanin-free ink from Sepia officinalis, which was monitored by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) techniques. The melanin-free ink contains a strong tyrosinase activity with pH 7 and 58?°C as optima of pH and temperature, respectively. Such activity is stimulated by ferrous ions and strongly inhibited by Mn²⁺, EDTA, H₂O₂, arbutin, and p-coumaric acid. We also show that 2 Mercapto-ethanol (14?mM) quickly and completely inactivated sepia tyrosinase. The melanin-free ink exhibits a major protein on SDS–PAGE with an N-terminal sequence matching perfectly with an internal sequence of the sepia peroxidase. The zymogram confirmed the inactive state of this truncated protein and the presence of an active tyrosinase enzyme. Interestingly, this activity was able to cross-link the β-casein protein. The tyrosinase implication in reticulation was demonstrated by the addition of its inhibitors, with 2-mercaptoethanol being the most effective, followed by arbutin, p-coumaric acid, and hydrogen peroxide.     

Study of microstructure, phases and microhardness of metallic coatings deposited by flame thermal spray

The recharging technique by thermal spraying offers the opportunity of renovating the worn surface parts of a machine element to give it again a new technical life despite its previous degradation in service. This process has consequently interesting economic impacts. In order to improve the adherence between 100Cr6 steel deposits and the substrate material (left worn crankshafts), company SNC ATRA of Béjaïa uses at present a composite formed by (100Cr6 steel/molybdenum bond coat of 0.2 mm thick/crankshaft substrate). As a matter of fact, it is shown in the present work that the molybdenum bond coat is not appropriate since, for the 0.2 mm thickness, lateral cracks are observed in the middle of the bond coat. On the other hand, our experiment is that a deposit of 100Cr6 steel projected directly on the substrate seems more promising since no gaps or cracks were detected at the “deposit/substrate” interface of this two-material composite. Lastly, phase analysis using X-ray diffraction confirmed that during spraying process, a stable -phase (bcc) of 100Cr6 wire was transformed to a new phase of γ-phase (fcc). The coatings exhibited the higher microhardness which would contribute to increase wear resistance.     

Surface damage of poly(methylmethacrylate) under fretting loading

The initial fretting damage in a glass/PMMA contact was investigated by means of experiments and numerical (F.E.M.) simulations. Both micro-crack nucleation at the contact edges and particle detachment were identified on the PMMA's surface. Micro-crack initiation was related to the combination of high tensile stresses and positive hydrostatic pressures which are known to enhance crazing. During the early stages of the fretting tests, the distribution of the detached particles within the contact was correlated to the spatial distribution of the cumulative interfacial energy dissipated by friction. As the number of cycles was increased, it was observed that detached particles moved toward the middle of the contact. On the basis of FEM simulations, this particle displacement within the contact was attributed to the existence of differential micro-displacements during the fretting cycle.     

Phase structure and mechanical properties of PP/EPR/CaCO3 nanocomposites: Effect of particle's size and treatment

Calcium carbonate (CaCO₃) reinforced polypropylene/ethylene propylene rubber (PP/EPR) copolymer composites for automotive use were developed by means of extrusion and injection molding process. Three kinds of CaCO₃ (stearic acid treated and untreated) nanoparticles and microparticles were used as fillers. The influence of stearic acid, particle size, and filler content on the state distribution and morphology were investigated by SEM and rheological measurements. Two different morphologies were observed: EPR and CaCO₃ dispersed in the PP matrix and a core shell structure, depending on the interactions between EPR and CaCO₃. Toughening mechanisms and mechanical properties of the different systems were investigated. Significant improvement in tensile modulus is observed in all composites, depending on filler content. Elongation and notched impact strength were drastically decreased, especially for composites with nano CaCO₃. Better impact properties were obtained with low content of treated particles, showing the importance of filler treatment. POLYM. ENG. SCI., 55:2859–2868, 2015. © 2015 Society of Plastics Engineers     

LC/ESI-MS/MS characterisation of procyanidins and propelargonidins responsible for the strong antioxidant activity of the edible halophyte Mesembryanthemum edule L.

Mesembryanthemum edule is used as a food ingredient and in traditional medicine. In this study, we investigated antioxidant activities of several extracts (methanol/acidified water, v/v: 20/80; 40/60 and 60/40) obtained from M. edule leaf, stem and root. Then, individual phenolics were characterised by reverse-phase HPLC coupled to electrospray ionisation mass spectrometry and multi-stage MS fragment analysis. Results showed that 40% methanol leaf extract, 40% methanol root extract and 20% methanol stem extract displayed the highest scavenging activity against DPPH and ABTS radicals. Regarding LC/ESI-MS/MS identification of active phenols, there were significant differences among the fractions of interest. In fact, 40% leaf extract mainly contained procyanidins, whereas propelargonidins were the major phenolics in 20% methanol stem extract, while, in 40% root extract, the active compounds remained unidentified. These results indicate that edible M. edule can be used as a nutraceutical in the pharmaceutical industry.     

Municipal solid waste compost application improves productivity, polyphenol content, and antioxidant capacity of Mesembryanthemum edule

Organic wastes were successfully used as soil amendment to improve agrosystems productivity. Yet, the effectiveness of this practice to enhance plant antioxidant capacities has received little attention. Here, we assess the effect of municipal solid waste (MSW) compost (at 40 t ha(-1)) on growth, polyphenol contents and antioxidant activities of Mesembryanthemum edule. MSW compost application significantly increased the soil contents of carbon, nitrogen, calcium, phosphorus and potassium. This was associated with higher nutrient (N, P, and K) uptake, which likely led to the significant improvement of the plant biomass and relative growth rate (RGR) (+93% on average) as compared to the control. In the same way, the fertilizing effect of the added organic matter significantly enhanced the antioxidant potential M. edule, assessed by radical scavenging activity, iron reducing power and β-carotene bleaching capacity. This was associated with significantly higher antioxidant contents, mainly total phenols and flavonoids. Heavy metal (Pb, Cd, Cu, and Zn) concentrations were slightly increased upon compost application, but remained lower than phytotoxic values. Overall, our results point out that short-term MSW compost application at 40 t ha(-1) is efficient in enhancing the productivity together with the antioxidant potentiality of M. edule without any adverse environmental impact.     

Radiation induced carbon complexes in gallium arsenide

Gallium arsenide grown by the metallorganic chemical vapour deposition method and n-doped to various silicon concentrations was irradiated with reactor neutrons (1 MeV equivalent damage in silicon) in the fluence range 0 to 3 × 10¹⁵ cm⁻². Native defects, including carbon which is a residual impurity of the growth method, and those introduced by irradiation, were characterized by photoluminescence (PL) and deep level transient spectroscopy (DLTS). In some samples with fixed doping value, the PL intensity of all the transitions, including that to the carbon impurity increases at low fluence levels before decreasing at high fluence. At higher fluences, the transition to the carbon impurity goes through other maxima. The carbon PL intensity versus fluence curve depends on initial doping. DLTS results reveal the removal of a trap EL12 at low fluences, but the introduction of other traps at higher fluences. The defect introduction rates depend on fluence. We attribute the variation in the carbon PL intensity to an interaction between the defects introduced by the irradiation and the carbon impurity.     

Increase in the efficiency of the speed controller of an induction machine drive by means of artificial intelligence

This paper describes the application of artificial intelligence to the synthesis of an efficient speed controller for an indirect field-oriented induction machine drive. In this case, two methods of artificial intelligence are proposed to reproduce the characteristics of a fuzzy controller with the maximum number of fuzzy sets. The first method is the adaptive network-based fuzzy inference system (ANFIS) structure by Sugeno and the second is the artificial neural network (ANN) structure. The resulting characteristics of each controller are presented.     

Ultrasonic Velocity as a Tool for Physical and Mechanical Parameters Prediction within Geo-Materials: Application on Cement Mortar

This paper discusses the ability of ultrasonic wave velocity to evaluate some physical parameters within mortar. The behavior of ultrasonic pulse velocity within mortar subjected to incremetal stress was also studied. For experimentation, we carried out ultrasonic measurements on mortar samples before and during uniaxial compressive strength, perpendicularly to the stress direction. The water/cement ratios were varied in order to contribute certain specific characteristics. A set of expressions was obtained linking the initial velocities of longitudinal ultrasonic waves with compressive strength, density, porosity and load at elastic limit.The evolution of ultrasonic velocity through mortar samples under continuous incremental uniaxial stress were also investigated. The results illustrated the behavior of ultrasonic pulse velocity as a function of the applied stress. It was observed that velocity did not decrease under initial loading and until about 70% of the ultimate stress, where sudden decrease occurred, followed by the failure of the material.