FeNO评估吸入ICS+LABA对变应性咳嗽患者治疗效果的价值研究

本研究旨在评估FeNO对AC患者吸入ICS+LABA治疗的临床价值。     

声带曲霉菌感染伴侵袭性肺曲霉菌感染1例

<正>病例资料患者李XX,女,48岁,因"咳嗽咳痰3月"入院。患者3月前受凉后出现阵发性咳嗽,咳少量白痰,昼夜频率一致,感畏寒发热,自测体温最高38.0℃,有咽痛及声音嘶哑,无胸闷气短,无夜间盗汗,当地社区医院予输液(具体药物不详)治疗1周,效果不佳。01月26日至苏北人民医院就诊,查耳鼻喉镜提示:双侧声带全层见白色角化样突起(图1);胸部CT提示两肺炎症(图2);02月05日至南京鼓楼医院耳鼻喉科就诊,喉镜取声带肿物病理提示黏膜组织鳞状     

von Hippel-Lindau(VHL)的表达、纯化及结合活性分析

目的纯化人von Hippel-Lindau(VHL)基因重组蛋白并进行功能鉴定。方法采用PCR从人乳腺c DNA中获得VHL基因序列,该序列被插入到原核表达载体p GEX-KG中,得到谷胱甘肽巯基转移酶(GST)-VHL重组质粒,将其转化至BL21(DE3)感受态细菌,经小量诱导后,采用SDS-PAGE和Western blot法检测该蛋白表达情况,使用GST微珠纯化该重组蛋白,利用GST pull-down技术验证其功能。结果获得的重组质粒可成功双酶切,基因测序表明VHL序列正确且无突变;将其转化至BL21(DE3)感受态细菌并小量诱导,纯化得到相对分子质量(Mr)约56 000的重组蛋白; GST pull-down技术证明GST-VHL重组蛋白在体外具有结合缺氧诱导因子1α(HIF-1α)的功能。结论纯化出GST-VHL重组蛋白并可与HIF-1α蛋白在体外结合。     

前纵隔恶性孤立性纤维瘤致上腔静脉综合征并胸腔积液1例

孤立性纤维性肿瘤(solitary fibrous tumor,SFT)在临床上较为少见,是一类间质源性肿瘤,起源于树突状间质细胞。随着病情进展少数情况下可引起副瘤综合征。我院诊治前纵隔恶性孤立性纤维性肿瘤致上腔静脉综合征并胸腔积液1例,现将临床资料汇报如下。1临床资料患者男性,31岁,因"胸闷、胸痛半月余"于2014年07月31日入院。查体:体温36.8℃,睑结膜稍充血,面颈部潮红,左     

神经干细胞在新型复合支架中的生长和分化

BACKGROUND:Neural stem cells with self-proliferation and differentiation potential are the ideal seed cells for central nervous tissue engineering. Although collagen and silk fibroin as biological scaffold materials have been widely used, both of them used alone have certain shortcomings. Is it possible to combine the two materials to build a novel neural tissue-engineered scaffold? What is the effect of this novel scaffold on the growth and differentiation of neural stem cells? OBJECTIVE:To observe the growth and differentiation of neural stem cells seeded onto the novel composite scaffold. METHODS:The rat embryonic neural stem cells were inoculated onto new composite scaffolds, and then, their growth and differentiation were observed by light microscopy and scanning electron microscopy. Neural stem cells were cultured in conventional suspension culture as control group. Cell counting kit-8 assay was used to detect viability of neural stem cells in the two groups. Three-dimensional composite scaffolds carrying neural stem cells were sliced into paraffin sections to observe the growth and differentiation of neural stem cells by hematoxylin-eosin staining and immunofluorescence staining. RESULTS AND CONCLUSION: Neural stem cells cultured on the new composite scaffold grew and differentiated well, and interconnected synapses were observed. Cell counting kit-8 assay showed that neural stem cells on the scaffold grew well, and the cell viability was significantly higher in the composite scaffold group than that in the control group (P < 0.05). Hematoxylin-eosin staining and immunofluorescence staining of paraffin sections further provided evidence for good growth and differentiation of neural stem cells on the scaffold. These results indicate that the novel composite scaffold with good biocompatibility benefits the growth and differentiation of neural stem cells, promising a favorable application prospect.     

一例烧伤患者感染炭疽杆菌的调查报告

1997年1月我站接到本市某医院一例烧伤病人感染炭疽杆菌的报告，即进行了流行病学调查及病原学分离鉴定，现将结果报告如下。1流行病学调查及处理1．1发病经过：患者包某，女，21岁，安徽来疆无业人员，以拾破烂为生，所居住的房屋原为补汽车轮胎工房，患者常用工房内剩有的橡胶皮和补胎胶水等物品生火。1月6日患者在生火时因火不着故往炉堂内倒入一些补胎胶水，以致引起火炉爆炸，造成面部、双手及双下肢被火烧伤，当日送医院抢救。在治疗过程中，发现烧残的上肢结痴变黑，周围有水肿，并伴有高热，即采集该部位渗出物作分离培养，检出可疑…     

延迟性脾破裂30例分析

目的：总结延迟性脾破裂的诊断和治疗。方法：回顾分析2000年1月-2005年12月收治的30例患者。讨论延迟性脾破裂的概念、诊断及外科治疗。结果：保守治疗痊愈6例，保守治疗无效继发大出血而手术4例，急诊手术20例，均行脾切除术，死亡1例。结论：延迟性脾破裂往往在受伤时即有脾损伤，而诊断却在48小时之后，易致误诊。诊断时需仔细询问病史，严密动态观察病情及有效辅助检查，根据患者的不同情况，选择不同的治疗方法。     

Flag-Tinagl1真核表达载体的构建及功能验证

目的 构建带Flag标签的肾小管间质性肾炎抗原样蛋白1(Tinagl1)基因真核表达载体,检测其对肝癌细胞HepG2 ERK/AKT磷酸化、生长和迁移的影响.方法 以人乳腺cDNA文库为模板,PCR扩增Tinagl1基因片段,将其插入pcDNA3.0载体,经双酶切和测序验证后,将重组质粒转染到肝癌HepG2细胞中,采用Western印迹检测重组蛋白对ERK和AKT磷酸化水平的影响,生长曲线和划痕实验检测其对肝癌细胞HepG2生长和迁移的影响.结果 双酶切和Western印迹结果表明,pcDNA3.0-Flag-Tinagl1真核表达质粒构建成功,Tinagl1可降低ERK和AKT的磷酸化水平;生长曲线和划痕实验表明Tinagl1抑制肝癌细胞HepG2生长和迁移.结论 构建了带Flag标签的Tinagl1真核表达载体,并能抑制肝癌细胞HepG2中ERK/AKT磷酸化、生长和迁移,为进一步研究Tinagl1在肝癌细胞中的功能奠定了基础.     

表没食子儿茶素没食子酸醋对人胃癌细胞迁移、侵袭和cripto蛋白表达的影响

目的观察表没食子儿茶素没食子酸醋(EGCG)对人胃癌细胞迁移、侵袭的影响,并探讨其可能机制。方法将人胃癌BGC823细胞分为两组,实验组加入20、40、80μmol/L的EGCG处理,以RPMI1640代替EGCG处理细胞作为空白对照组,采用划痕试验法检测癌细胞迁移能力,以Transwell法检测癌细胞侵袭能力,用免疫荧光方法检测癌细胞cripto基因蛋白水平变化。结果实验组细胞迁移距离、细胞穿过滤膜个数、cripto基因蛋白阳性率明显低于对照组,且均呈剂量依赖性,P均<0.05。结论 EGCG可明显抑制胃癌BGC823细胞迁移和侵袭能力,其机制可能与下调cripto基因表达有关。