用石英晶体微天平法研究聚乳酸的酶降解过程

利用石英晶体微天平(QCM)探讨影响聚乳酸降解速率的不同因素。聚乳酸的酶降解分两步进行：酶吸附到聚乳酸的表面和聚乳酸在酶作用下的降解。聚乳酸的降解初速率随酶加入量的增加而增大,但达到一定的加入量之后,降解初速率接近一个常数;当pH为7~9时,聚乳酸的降解初速率随pH的增大而增大;当温度为25~40 ℃时,降解初速率随温度的升高而增大。     

《军医进修学院学报》2005-2009年载文及引文分析

目的观察《军医进修学院学报》（简称《学报》）2005-2009年刊登论文的各项信息指标,为研究期刊发展方向提供依据。方法采用文献计量学方法对《学报》期刊信息量指数、引证文献信息（引文数量、引文率）、不同栏目年载文量和基金论文量进行统计。结果2005-2009年《学报》5年平均载文信息量指数为0.56,总引文率从4.96增至8.19,载文量从270篇增至456篇,增幅达69%,基金论文从62篇增至156篇,增幅达152%。结论加大组稿、约稿力度以及增加国家、军队和省部级基金课题收录比例,有助于《学报》提高期刊质量和维系期刊可持续发展。     

中国战伤博物馆的建设与意义

中国战伤博物馆是国内唯一的军事医学博物馆,收藏特种武器、常规武器、高新技术武器,以及军事环境和军事作业对人体和生物损伤的病理标本、实物和文件档案,共计4350件。中国战伤博物馆的建设对于军事医学教学和培训、公共卫生知识普及、保存军事医学史料、对外开放等方面具有重要意义,中国政府和军队有关部门应当予以高度重视。     

Different metabolic profiles of K1 serotype and non-serotype K1 and K2 Klebsiella pneumoniae isolates in oral infection mice model

K1 or K2 serotype Klebsiella pneumoniae isolate caused clinical pyogenic liver abscess (KLA) infection is prevalent in many areas. It has been identified that K1 or K2 serotype K. pneumoniae isolates caused KLA infection in mice by oral inoculation. In our study, K1 serotype K. pneumoniae isolate Kp1002 with hypermucoviscosity (HV)-positive phenotype caused KLA infection in C57BL/6 mice by oral inoculation. Simultaneously, non-serotype K1 and K2 isolate Kp1014 with HV-negative phenotype failed to cause KLA infection in the same manner. It seems that gastrointestinal tract translocation is the pathway by which K1 or K2 serotype K. pneumoniae caused KLA infection. Liquid chromatography-tandem mass spectrometry was used to further analyze metabolic profile changes in mice with KLA infection. Data showed that after Kp1002 or Kp1014 oral inoculation, serum Phosphatidylcholine (PC) and Lysophosphatidylcholine (LPC) levels significantly changed in mice. Some PC and LPC molecules showed changes both in the Kp1002 KLA group and the Kp1014 no-KLA group compared with the control group. The level of 18:1/18:2-PC significantly changed in the Kp1002 KLA group compared with the control group, but showed no change between the Kp1014 no-KLA group and the control group. The level of 18:1/18:2-PC might have been particularly affected by KLA infection caused by K1 serotype K. pneumoniae Kp1002. It may be a potential biomarker for KLA infection.     

Relationship between Drug Release and Some Physical Parameters of Drug Sorption onto PLA Fibers

Drug release and its relationship with kinetic and thermodynamic parameters of drug sorption onto poly(lactic acid) (PLA) fibers have been studied using Diclofenac, 5-Fluorouracil (5-FU) and Metformin as model drugs. The sorption method is more flexible and avoids the damaged drugs, remaining toxic organic solvents and safety problems which occurred with the dissolution method. The quantitative relationship with high correlation between drug-release and drug-loading concentration, affinity and activation energy for diffusion has been established to predict the initial burst and subsequent release of the drugs. Drugs with higher activation energy for diffusion, lower diffusion coefficients and higher affinity on PLA fiber, such as Diclofenac, can achieve high loading capacity and constant drug release. It has also been found that elevated temperatures can achieve high loading capacity and constant release. In addition, the study showed that Diclofenac release profiles were similar for sorption and dissolution loading methods.     

人体胆囊结石时胆囊组织PGE、PGI2、LTC4的含量

本文测定非结石组（ｎ＝１６）与结石组（ｎ＝４７）人体胆囊粘膜内前列腺素Ｅ（ＰＧＥ）、前列环素（ＰＧＩ２）白三烯０４（ＬＴＣ４）的含量及磷脂酶Ａ２（ＰＬＡ２）的活性，发现结石组四者的水平均非常显著地高于非结石组。提示可能由于脂类代谢紊乱，胆囊组织的ＰＬＡ２活性增加．致使ＰＧｓ、ＬＴｓ水平增高，引起胆囊上皮细胞活化，合成分泌粘蛋白功能亢进，从而参与促进结石的形成。     

肺炎支原体感染的血清学检测方法比较

目的比较几种检测方法对呼吸道感染患者血清肺炎支原体（MP）特异性抗体IgM检测的检出率。方法以冷凝集试验（CAT）、明胶颗粒凝集法（PLA）、酶联免疫吸附试验（ELISA）3种方法对95例呼吸道感染患者血清肺炎支原体IgM抗体进行检测。结果冷凝集试验、明胶颗粒凝集法、酶联免疫吸附试验检测血清肺炎支原体IgM抗体的敏感性分别为69.1%、96.4%和90.9%,特异性分别为75%、100%和90%,准确度分别为68.4%、97.9%和90.5%。用配对资料四格表法对三种方法的检出率进行分析,经SPSS软件统计分析,冷凝集试验与明胶颗粒凝集法和酶联免疫吸附试验相比较,差异有统计学意义（P〈0.05）,酶联免疫吸附试验与明胶颗粒凝集法相比较,差异无统计学意义（P〉0.05）。结论冷凝集试验与另外两种方法相比有显著差异,而酶联免疫吸附试验与明胶颗粒凝集法两种方法没有显著差异,两者联合应用可以提高阳性率,对临床治疗起到及时的指导。     

军队医学图书馆文献资源的共建共享

阐述了军队医学图书馆文献信息资源共建共享的意义及开展的情况,提出了深入发展军队医学图书馆文献信息资源共建共享的建议。     

军队医药卫生科技查新管理信息系统及其存在的问题

分析了科技查新管理系统的应用现状,从系统的功能设计、系统的关键技术和系统的应用三方面探讨了军队医药卫生科技查新管理信息系统的研发,并针对信息沟通不畅和查新信息数据不完整等问题,提出了构建及完善"查新管理"、"资源管理"、"经费管理"、"查询分析"、"统计分析"、"系统维护"及"系统管理"等数据模块,提高军队医药卫生科技查新管理信息系统应用研究水平。     

军队院校图书馆的现状及其可持续发展的措施

对军校图书馆数字化及资源共建共享、人员及经费现状进行了分析,并就实现军队院校图书馆可持续发展提出了建议.