军队人员口腔卫生观念与行为的问卷调查及分析

目的为了解我军部队人员口腔卫生观念与行为情况,探索口腔护理保健现状。方法采取分层抽样方法,分别对口腔健康观念与行为情况进行问卷调查、填写记录。结果共调查陆、海、空官兵1269人,每13早晚2次刷牙率为75．02％;不同军种、不同军衔官兵的牙科检查需求现状均不同;口腔疾病未治疗的原因,无口腔科医生占25．84％,工作忙没有时间占47．75％,认为牙齿好坏无所谓占4.41％,不清楚是否需要占21．98％。结论进行口腔护理教育、定期口腔检查和早期口腔医疗对我军部队官兵非常必要。     

新型高孔隙率聚乳酸支架复合物在体内形成新生软骨的实验观察

目的观察软骨细胞-新型高孔隙率聚乳酸(PLA)支架复合物在体内形成新生软骨的能力.方法体外培养兔耳廓弹性软骨细胞,取第2代软骨细胞与新型高孔隙率聚乳酸支架形成复合物,体外培养1周后移植于兔颈部气管旁带状肌间隙,以未种软骨细胞的聚乳酸支架作为对照.结果移植于兔体内1个月后,接种了软骨细胞的新型高孔隙率PLA支架有新生的软骨形成,2个月后新生软骨逐渐成熟并形成明显的软骨陷窝,而对照组无软骨形成.结论这种新型聚乳酸支架种植软骨细胞后在体内具有成软骨能力,可用作软骨组织工程的支架材料.     

地塞米松影响血小板聚集、释放机理的探讨

通过对离体兔血小板研究发现:地塞米松(Dex)能抑制胶原诱导的血小板聚集、释放和血栓素A_2(TXA_2)生成等反应,而对花生四烯酸(AA)诱导的上述反应则无明显影响。本研究还发现,Dex能抑制血小板的磷脂酸A_2(PLA_2)活性,细胞外Ca~(2+)可部分拮抗Dex的上述作用,表明Dex的作用可能与其限制细胞外Ca~(2+)进入细胞内有关。Dex对血小板钙调素(CaM)和腺苷酸环化酶(AC)活性,以及对cAMP和cGMP的生成均无明显影响。     

Exploration of associations between phospholipase A2 gene family polymorphisms and AIDS progression using the SNPlex method.

Members of the secreted phospholipase A2 (PLA2) protein family can inhibit HIV-1 virus replication in vitro. To evaluate the impact of PLA2 gene polymorphisms on AIDS disease development, we studied 12 family members using SNPlextrade mark technology that permitted simultaneous typing of 70 tagging Single Nucleotide Polymorphisms (tagSNPs). The study utilized HIV-1 seropositive donors with slow progressor (n=168) or rapid progressor (n=54) status, plus 355 control subjects. All donors were Caucasian (total 577 individuals). Genetic associations yielded mainly 0.01PLA2G2A, PLA2G2D and PLA2G3) and cytosolic (PLA2G4A and PLA2G6) PLA2 genes, including three (PLA2G2A, PLA2G2D and PLA2G4A) implicated in the pathogenesis of other diseases. Our results suggest that the PLA2 gene family may represent genes of interest for a larger study targeting all the known tagSNPs in the PLA2 genes. The data presented in this study will have to be confirmed in other AIDS cohorts and will also be useful for studies undertaken on the PLA2 gene family in other disease cohorts.     

Clinical study and PLA2G6 mutation screening analysis in Chinese patients with infantile neuroaxonal dystrophy

Background and purpose:  Infantile neuroaxonal dystrophy (INAD) is a rare autosomal recessive neurodegenerative disorder. The most typical neuropathological finding of this disease is axonal swelling. Before the identification of associated mutations in PLA2G6 -encoding iPLA₂-VIA (cytosolic Ca²⁺-independent phospholipids A₂, group VIA) in 2006, neuropathological evidence was critical for definitive diagnosis. Only five genetic studies in INAD patients have been published worldwide, wherein 44 mutations were reported. To define the clinical and genetic characteristics of Chinese patients with INAD, 10 cases were analyzed.
Methods:  For 10 cases of INAD, extensive clinical investigations, neuropathological examination, and mutation screening in PLA2G6 were performed.
Results:  All cases displayed typical clinical features. Axonal swelling was found in skin or sural nerve biopsy specimens in three cases. Twelve PLA2G6 mutations were identified, nine of which were novel. These novel mutations include six missense, one abolishing the normal start codon, one nonsense, and one splice-site mutation.
Conclusions:  The nine novel mutations identified in this study suggest the uniqueness of the PLA2G6 mutation spectrum in Chinese patients, and greatly extends the spectrum of known mutations in INAD patients. In addition to pathological evidence, genetic analysis can inform definitive diagnosis of INAD.     

以驻港精神加强护理士官队伍建设的实践

本文提出以政治教育为核心，筑牢思想基础；以基础护理技能训练为主线，提升业务素质；以和谐军营为目标，加强日常管理；以遂行任务为牵引，提高整体素质，从而加强护理士官队伍全面建设的思路，快速提升了护理士官队伍综合素质。     

幽门螺杆菌对胆囊结石形成的影响

目的 探讨幽门螺杆菌(Hp)与胆囊结石形成之间的关系.方法 从胆囊结石患者胆囊黏膜中检测Hp,并测量阳性和阴性组中的IL-2、IL-4、自由基以及PLA2含量.结果 54例中检出Hp 15例(27.8%).阳性组IL-2表达为(3.34±1.39)μg/ml,明显低于对照组的(4.98±2.08)μg/ml;IL-4表达为(14.34±8.29)pg/ml,明显高于阴性组的(6.73±2.14)pg/ml;自由基表达为(20.980±3.711)nU/ml,明显高于阴性组的(15.15±3.73)nU/ml,PLA2活性表达为(872.37±70.02)μmol/(L·s),明显高于阴性组的(586.42±57.35)μmol/(L·s),差异均有统计学意义(P＜0.01).自由基与PLA2活性的增高同IL-2活性降低以及IL-4活性的增高呈明显正相关.结论 胆囊结石患者存在有Hp感染因素,并有可能通过IL及自由基、PLA2促进胆囊结石的形成.     

以科学发展观为指导，推进军队图书馆可持续发展

分析了制约图书馆可持续发展的主要因素,以科学发展观为指导思想,指出了军队图书馆实现可持续发展必须坚持改革创新.