小鼠骨髓间充质干细胞体外诱导向平滑肌样细胞分化过程中myocardin的表达

目地探讨血小板源性生长因子BB联合高浓度胎牛血清体外诱导骨髓间充质干细胞定向分化为平滑肌细胞的可行性及myocardin在此过程中的表达变化。方法采用全骨髓贴壁法分离骨髓间充质干细胞,血小板源性生长因子BB(50μg/L)联合高浓度胎牛血清诱导骨髓间充质干细胞,分别于诱导前,及诱导4d和8d后用免疫细胞化学法检测细胞平滑肌肌动蛋白、平滑肌肌球蛋白重链和myocardin的表达。结果免疫组织化学显示,诱导前及诱导4d和8d时,平滑肌肌动蛋白、平滑肌肌球蛋白重链和myocardin三种蛋白的表达逐渐增强,平均灰度值逐渐降低,不同时间点之间比较差异有显著性(P<0.05)。结论血小板源性生长因子BB联合高浓度胎牛血清可有效诱导骨髓间充质干细胞向平滑肌样细胞分化。Myocardin在此分化过程中可能起了重要作用。     

心肌素在平滑肌细胞分化及表型调节中的作用

平滑肌细胞在动脉粥样硬化的发生和进展过程中起了重要作用。粥样斑块中的平滑肌细胞不仅来源于中膜平滑肌,还可以从骨髓干细胞分化而来。平滑肌细胞的分化是高度可逆的,并在多种局部环境因素的参与作用下发育。平滑肌细胞表达多种标志表明它们在分化成熟中的相对状态。但是没有单个细胞分化特异性标志能将平滑肌细胞与其它完全区别开。心肌素及与其相关的血浆反应因子共刺激因子、ETS家族三元复合物(EIK-1)和心肌素相关转录因子对平滑肌细胞分化起到一定作用。因此平滑肌祖细胞概念的提出,为动脉粥样硬化防治提供了一种新的治疗策略。     

The Mechanism of Resistance of Pseudomonas Aeruginosa toβ-lactam Antibiotics and Clinical Significance

Pseudomonas aeruginosa was one kind of con-ditional pathogenic bacteria.In recentyears,ithasbecome more and more resistantto a lotof kinds ofantibiotics commonly used clinically,in particular,toβ- lactam antibiotics. Researchers in the worldhave investigated the resistant mechanism of P.aeruginosa and proposed that the permeability ofouter membrane,β- lactamase and penicilline bind-ing proteins mightbe involved. We collected 30 re-sistant strains of P. aeruginosa isolated clinically,studied…     

The Value of p63 and CK5/6 Expression in the Differential Diagnosis of Ductal Lesions of Breast

Differentiation between benign and malignant lesions has always a problem in the pathological diagnosis. Presence of myoepithelial cells (MECs) is an vital indi- cator for existence of cancer or invasion[1, 2]. Although these histological and morphological features have been well-depicted, it is still difficult to determine the pres- ence of MECs in breast lesions and to evaluate the nature of a lesion by routine HE staining under light microscope. So it is of importance to find useful mark…     

HepG2细胞系载脂蛋白B表达的调控研究

探讨白细胞介素－11（IL－11）及乙醇对人肝肿瘤细胞系（HepG2）载脂蛋白B（apoB）mRNA表达及蛋白分泌的作用。培养HepG2细胞,分组加入不同浓度IL－11进行浓度试验,收集培养液及细胞。用火箭电泳法及Northernblot分别检测培养液中apoB含量及HepG2细胞apoBmRNA的表达。IL－11及乙醇抑制试验按同法分组进行。结果IL－11能抑制HepG2细胞apoB表达与分泌,且呈量效正相关。IL－11能明显抑制乙醇的正向作用。说明IL－11能调节HepG2细胞apoBmRNA表达与分泌,可参与调节机体脂质代谢。     

hTK1在胃癌及癌前病变组织中的表达及其预后判断的相关性

目的探讨胃癌组织及癌前病变组织中hTK 1的表达及与细胞增生核抗原K i-67的关系,并探讨其对胃癌早期诊断及预后判定的价值。方法随机收集胃癌标本65例,不典型增生53例,正常胃黏膜7例,采用免疫组化S-P法检测hTK 1、K i-67的表达,并结合胃癌的临床病理特征进行分析。结果在正常胃黏膜、不典型增生和胃癌组织中,hTK 1的阳性表达率分别为0%、41.5%和78.4%;K i-67的阳性表达率为0%、47.2%和83.0%。癌组织和不典型增生组织中hTK 1与K i-67的阳性表达率占正常胃黏膜比较差异有显著性(P<0.01);癌组织中hTK 1与K i-67的阳性表达率与不典型增生组织比较差异有显著性(P<0.01);在不同程度的不典型增生和不同分化程度的胃癌各组间差异有显著性(P<0.05),而与浆膜浸润和淋巴结转移等生物学行为差异无显著性;hTK 1与K i-67表达具有显著相关性(P<0.05)。结论hTK 1的表达在胃癌的发生、发展过程中具有重要的作用,其表达与细胞增殖密切相关,是肿瘤细胞增殖指数的良好标记物;对于判断胃癌恶性程度及治疗效果和预后判断具有重要意义。     

恶性上皮性间皮瘤免疫组化特征及其鉴别诊断

目的　探讨恶性上皮性间皮瘤 (MEM)的免疫组化特征 ,寻找MEM与转移性卵巢腺癌 (OA)和结直肠腺癌(CA)鉴别诊断的免疫组化标记物。方法　采用免疫组化SP法染色 ,观察 18例MEM、2 0例OA和 2 0例CA组织标本中HBME 1、E cadherin、CA19 9、MOC 31和CK7的表达。结果　E cadherin、CA19 9和MOC 31的表达在MEM与OA两组间差异显著 (P <0 0 5 )。HBME 1、E cadherin、CA19 9、MOC 31和CK7的表达在MEM与CA两组间差异极显著(P <0 0 1)。结论　HBME 1可作为MEM与CA鉴别的阳性标记物 ;E cadherin、CA19 9和MOC 31均可作为MEM与OA和CA鉴别的阴性标记物 ;CK7是鉴别MEM与CA最好的阳性标记物 ,但不能作为MEM与OA鉴别的指标。     

我国心血管病理学近十年的回顾与展望

近十年来，我国心血管疾病发病率有所增高，我国心血管病理学基础与临床研究迅速发展，研究水平不断提高，从动物实验到人体研究，从大体到组织、细胞、分子以及基因，研究手段丰富多样，研究技术日臻成熟。《中华病理学杂志》见证了我国心血管病理学近十年的发展历程。     

Anisodamine Inhibits Engotoxin—induced Tissue Factor Expression in Human Endothelial Cells

By study on the effect of anisodamine on lipopolysaccharide-induced expression of tissue factor(TF) in vascular endothelial cells (EC),the mechanism of anisodamine antithrombosis,as well as in the treatment of bacteraemic shock was investigated.Human umbilical vein endothelial cells (HUVECs) were cultured by trypsin digestion method.TF activity was measured in the lysates of HUVEC by using a single step clotting assay.Specific mRNA expression was detected by Northern blotting.In order to evaluate a possible contribution of the nuclear factor (NF)-κB pathway on the effects observed,electrophoretic mobility shift assays (EMSA) were performed using nuclear extracts from HUVECs and NF-κB-binding oligonucleotides.The results showed that treatment of HUVEC with LPS resulted in a significant increase in TF activity.Anisodamine dose-dependently inhibited LPS-induced upregulation of TF.These effects was also confirmed on the level of specific TF mRNA expression by Northern blotting.Furthermore,EMSA showed that anisodamine completely abolished LPS-induced NF-κB DNA binding activity in nuclear extracts from HUVECs treated with LPS together with anisodamine.The results suggest that anisodamine counteracts endothelial cell activation by inhibiting LPS-induced TF expression in these cells.Its interference with the NF-κB pathway might-at least in part-contribute to this effect.The ability of anisodamine to counteract LPS effect on endothelial cells might be one underlying mechanism explaining its antithrombosis and efficacy in the treatment of bacteraemic shock.     

低密度脂蛋白诱导动脉平滑肌细胞表达MIP-1αmRNA

目的探讨天然和氧化低密度脂蛋白(n-LDL,ox-LDL)能否诱导培养的兔主动脉平滑肌细胞表达巨噬细胞炎性蛋白(MIP)-1α mRNA.方法将培养的兔主动脉平滑肌细胞暴露于n-LDL、ox-LDL后,分别用原位分子杂交法及逆转录聚合酶链反应(RT-PCR)检测其MIP-1α mRNA的表达.结果培养的兔主动脉平滑肌细胞能表达低水平的MIP-1α mRNA,天然及氧化低密度脂蛋白能增强平滑肌细胞表达MIP-1α mRNA,组间差异有极显著性意义(P＜0.01).结论n-LDL和ox-LDL通过诱导平滑肌细胞表达MIP-1α,从而在动脉粥样硬化早期病变的形成过程中起重要作用.