Pi (Spleen)-Deficiency Syndrome in Tumor Microenvironment Is the Pivotal Pathogenesis of Colorectal Cancer Immune Escape

Cancer immunoediting consists of three sequential phases: elimination, equilibrium, and escape. For colorectal adenoma-carcinoma sequence, the adenoma dysplastic progression may represent an equilibrium phase and the cancer stage as escape phase. Immune system eliminates transformed enterocytes by destroying them at first, sculpts them at the same time and selects the variants subsequently that are no longer recognized and insensitive to immune effectors, and finally induces immunosuppressive state within the tumor microenvironment that facilitates immune escape and tumor outgrowth. Immunosuppression and inflammation are the two crucial features of Pi(Spleen)-deficiency. Classic quotations, immune evidence and clinical observations suggest that Spleen(but not other organs) deficiency is the key pathogenesis of colorectal cancer(CRC) microenvironment. Weakness of old age, immunosuppressive cytokines from chronic inflammation, tumor-derived immunosuppressive factors and surrendered immune cells—regulatory T cells, myeloid-derived suppressor cells and tumor associated macrophages(TAMs) constitutes CRC microenvironment of Pi-deficiency. Furthermore, excess in superficiality, such as phlegm stagnation, blood stasis and toxin accumulation are induced by chronic inflammation on the basis of asthenia in origin, an immunosuppressive state. Great masters of Chinese medicine emphasize that strengthen Pi is the chief therapeutic principle for CRC which receives good therapeutic effects. So, Pi-deficiency based syndrome is the pivotal pathogenesis of tumor microenvironment. The immunosuppressive microenvironment facilitates immune escape which play an important role in the transition from adenoma to adenocarcinoma. There are some signs that strengthen Pi based treatment has potential capacity to ameliorate tumor environment. It might be a novel starting point to explore the mechanism of strengthen Pi based therapy in the prevention and treatment of CRC through regulation of tumor environment and immunoediting.     

中西医结合治疗SARS临床研究

面对SARS严峻的挑战,中医药工作者积极参与临床救治,积累了初步的临床经验.中西医结合治疗是否有利于提高SARS的临床疗效成为世界瞩目的问题.这已经不仅仅是个科学问题,而且是关系到如何发挥中医药的优势,尽早解除SARS对广大人民生命威胁的重大现实问题.     

利用siRNA抑制人骨肉瘤细胞survivin的表达

目的构建生存素(survivin)短发夹状RNA表达载体,检测其对骨肉瘤细胞survivin mRNA及蛋白表达的影响。方法体外构建survivin shRNA表达载体pSilence2.1-neo-survivin,转染骨肉瘤细胞系MG-63,RT-PCR、免疫组化方法检测转染前后survivin mRNA及蛋白的表达,MTT比色法检测细胞增殖活性。结果pSilence2.1-neo-survivin载体转染能显著抑制survivin mRNA、蛋白表达,转染后48h细胞增殖的抑制率为61.83%。结论pSilence2.1-neo-survivin可显著抑制MG-63细胞survivinmRNA、蛋白的表达及细胞的增殖活性。     

第一跖趾关节融合联合二至五跖趾关节成形治疗类风湿关节炎致前足畸形

 目的 探讨采用第一跖趾关节融合联合二至五跖趾关节成形治疗类风湿关节炎致前足畸形的效果。方法 回顾性分析2007年6月至2010年10月采用第一跖趾关节融合联合二至五跖趾关节成形治疗19例(35足)类风湿关节炎致前足畸形患者资料,男2例(4足),女17例(31足);年龄33～73岁,平均56岁。患者均有不同程度外翻锤状趾畸形和跖痛。采用美国足踝外科协会(American Orthopaedic Foot and Ankle Society,AOFAS)趾、跖趾、趾间关节评分及视觉模拟(visual analogue scale,VAS)评分评价手术效果。在术前及术后X线片上测量外翻角(hallux valgus angle,HVA)及第一、二跖骨间角(intermetatarsal angle,IMA),了解畸形矫正情况。结果 术后17例(32足)患者获得平均42个月随访,患足外形均得到不同程度改善;29足跖痛完全消失;3足出现第五跖骨外侧转移性跖痛,经垫前足减压垫缓解。1足因趾末节部分坏死而切除。成形的跖趾关节均有不同程度僵硬。AOFAS评分、VAS评分、HVA及IMA度数,术前分别为(46．82±6．13)分、(9.03±1.82)分、38.96°±10.13°、15.87°±3.43°,末次随访时为(84.25±2.87)分、(2.12±0.67)分、15.84°±5.12°、10.35°±1.67°。根据AOFAS评分,优23足,良5足,可4足,优良率为87.5%(28/32)。结论 第一跖趾关节融合联合二至五跖趾关节成形治疗类风湿关节炎致前足畸形效果优良,术后能明显矫正畸形,缓解疼痛,改善功能。     

剖宫产腹部切口脂肪液化20例诊治体会

目的:探讨剖宫产术后脂腹部切口脂肪液化的发生因素及处理方法。方法:采用回顾性研究方法分析在本院产科剖宫产术后腹部切口发生脂肪液化的20例患者临床资料,结果:孕妇肥胖、术后咳嗽、术前合并症(妊娠水肿、贫血)、前次剖宫产、滞产以及手术时间长、缝合方法不当、羊水刺激成为造成腹部切口脂肪液化的重要原因。结论:重视孕期保健,提高产科质量,降低剖宫产率,及时发现剖宫产术后腹部切口脂肪液化并予局部彻底清创引流,积极治疗后可有效治愈。     

RP—HPLC法同时测定注射用丹心宁中4个成份的含量

目的：建立同时测定注射用丹心宁中丹参素、原儿茶醛、丹酚酸B、红花黄色素含量的反相高效液相色谱法。方法：以Agilent Zorbax Extend C18柱（250mm×4．6mm,5μm）为固定相,乙腈-0．03％磷酸为流动相．在0～65min,乙腈量从2％上升到33％,检测波长为280nm和403nm,流速0．8mL·min^-1。结果：4种成份分离良好,丹参素、原儿茶醛、丹酚酸B、红花黄色素进样量分别在范围0．313—3．756μg,0．053-0．6362μg．0．567—6．809μg和0．0443—0．5321μg线性关系良好,加样回收率分别为丹参素102．1％,RSD=1．1％;原儿茶醛97．3％,RSD=1．2％;丹酚酸B99．3％,RSD=1．2％;羟基红花黄色素A100．6％,RSD=1．5％。结论：本法简单、快捷、适合于测定注射用丹心宁中丹参素、原儿茶醛、丹酚酸B、羟基红花黄色素A的含量。     

延长米索前列醇用药时间对药物流产结局影响的临床研究

目的：探讨延长米索前列醇用药时间对药物流产结局的影响。方法：将我院2012年300例宫内妊娠小于7周要求药物流产的妇女,常规服用米非司酮配伍米索前列醇的同时,延长米索前列醇口服用药400μg（观察组）,每日1次,连用3天;与未加用米索前列醇组（对照组）作疗效比较。结果：观察组完全流产率、平均阴道出血时间、阴道出血量与对照组相比差异均有显著性,不良反应两组相比差异无显著性。结论：在常规应用米非司酮片配伍米索前列醇的前提下,延长米索前列醇的用药时间可提高完全流产率,缩短出血时间,减少失血量,且不增加副反应,是一种方便、安全、简易、有效的补救方法,值得临床推广。     

FIA导数光度检测南瓜粉中多糖含量的研究

始载于明代李时珍<本草纲目>的南瓜,被中医认为性味甘温入脾胃二经,具有消炎止痛、解毒杀虫、补中益气等功效.主治消渴、哮喘、肺痛、疮肿、水火烫伤、脾胃虚弱等.     

搭建中医药共享实验平台,增强对外研发服务能力

随着知识经济和全球化时代的到来,进行科技基础条件的优化与整合,建立开放、共享的科技基础条件平台逐渐成为科技竞争的焦点之一,并在实现科技跨越式发展过程中起着非常重要的作用。《国家中长期科学和技术发展规划纲要(2006-2020年)》明确提出"科技基础条件平台,是科技创新的物质基础,是科技持续发展的重要前提和根本保障,通过有效配置