rlk/TXK encodes two forms of a novel cysteine string tyrosine kinase activated by Src family kinases

Rlk/Txk is a member of the BTK/Tec family of tyrosine kinases and is primarily expressed in T lymphocytes. Unlike other members of this kinase family, Rlk lacks a pleckstrin homology (PH) domain near the amino terminus and instead contains a distinctive cysteine string motif. We demonstrate here that Rlk protein consists of two isoforms that arise by alternative initiation of translation from the same cDNA. The shorter, internally initiated protein species lacks the cysteine string motif and is located in the nucleus when expressed in the absence of the larger form. In contrast, the larger form is cytoplasmic. We show that the larger form is palmitoylated and that mutation of its cysteine string motif both abolishes palmitoylation and allows the protein to migrate to the nucleus. The cysteine string, therefore, is a critical determinant of both fatty acid modification and protein localization for the larger isoform of Rlk, suggesting that Rlk regulation is distinct from the other Btk family kinases. We further show that Rlk is phosphorylated and changes localization in response to T-cell-receptor (TCR) activation and, like the other Btk family kinases, can be phosphorylated and activated by Src family kinases. However, unlike the other Btk family members, Rlk is activated independently of the activity of phosphatidylinositol 3-kinase, consistent with its lack of a PH domain. Thus, Rlk has two distinct isoforms, each of which may have unique properties in signaling downstream from the TCR.     

Impact of process variation on the RF and stability performance of SiGe source-based epitaxial layer TFET

Journal of Computational Electronics - In this study, the RF and stability aspects of SiGe source-based epitaxial layer tunnel field-effect transistor (SiGe source ETLTFET) are explored by...     

Deep Insight into the Noise Behavior of SiGe Source Based Epitaxial Layer Tunnel Field Effect Transistor

Silicon - A detailed simulation-based investigation on the noise behavior of SiGe source-based Epitaxial layer Tunnel field effect transistor (SiGe source ETLTFET) and Silicon Synthetic Electric...     

Thermal characteristics of arrays of swirling impinging jets: Effect of Reynolds number,impingement distance,and jet-to-jet separation

This paper numerically investigates both the inline and staggered arrays of circular multiple swirling air jets that impinge perpendicularly onto a smooth flat surface. The simulations were conducted for various flow and geometric parameters, such as Reynolds number (Re = 11,600, 24,600, and 35,000), jet-to-surface distance (H/D = 1, 2, 3, and 4), jet-to-jet separation distance (Z/D = 1.5, 3.0, and 4.5) and swirl numbers (S = 0, 0.3, and 0.75), where D is the nozzle diameter. For S = 0.75, a strong recirculation develops due to the vortex breakdown, depends on Z/D, around the axis and near the wall. The extent of the recirculation is larger for the staggered array. Intense heat transfer is anticipated at strong swirl and close impingement cases with the expense of uniformity; whereas a relatively even heat transfer is observed for larger impingements. The increase in jet-to-jet separation enhances the overall cooling effect and the staggered configuration of nozzles gives better performance. It appears that both the crossflow and turbulence around the periphery of each jet predominantly govern the heat transfer characteristics. The enhancement of heat transfer occurs for increasing Re, and the overall Nusselt number (Nu) prediction is scaled by Reⁿ, with n dependent on S. Finally a correlation is developed for the average Nusselt number to relate different control parameters.