从肺论郁

认为肺主治节是协调气机升降出入的重要机制，在肺主一身之气的基础上，通过司呼吸、宣发肃降、朝百脉、通调水道等，对人体的脏腑机能和气血津液的运行发挥推动和制约作用。治节失常则气机升降出入失调，郁证内生，临床常见表现有胸胁满闷，喜叹息；脘闷痞满，饮食不思；梅核气以及肺郁生痰的一些病症。主治当以宣肺开郁。     

东北地区人群慢性牙周炎与种植体周围炎的相关性

目的比较有慢性牙周炎（CP）病史的患者与牙周健康患者对种植体周围炎（PID）的易感性。方法选择2008-2009年于吉林大学第四医院口腔科接受种植治疗的74例牙齿缺失患者,按是否患有PID将其分为对照组（无PID,49例）和实验组（患有PID,25例）,每组中均包含牙周健康和既往患有CP的患者。分别对其进行系统牙周检查及问卷调查,并对结果进行统计学分析。结果结果中表明确诊PID的指标（探诊出血,自发出血,探诊深度,附着丧失）明显高于对照组,均有统计学意义;既往牙周健康的患者每5例中有1例患有PID,而既往患有CP的患者中每1.81例有1例患有PID,差异有统计学意义。结论有CP的病史的患者更易患PID。     

儿童口腔溃疡病诱发因素分析及护理对策

口腔溃疡可发生于儿童的任何年龄．为提高儿童口腔溃疡的治愈率．对2000～2002年来我院就诊的167例患儿进行诱发因素分析．并提出护理对策。     

新生儿、婴儿胃穿孔3例报告

新生儿、婴儿胃穿孔是较少见的疾病,往往由于延误治疗预后不良。现就我们治疗的三例报告如下: 例一,男,四天,系第一胎,孕35周早产(剖腹产)。婴儿取出后哭声响,面部青紫,经抢救二十分钟后,青紫消失,但随即出现阵发性呼吸困难。生后第二天开始腹胀,双下肢浮肿,呕吐,第四天转     

肝叶切除、肝动脉结扎治疗肝破裂术后继发股道出血一例报告

儿童肝内型胆道出血在临床上十分少见因肝外伤继发胆道出血者则更属罕见。现就最近我院收治的一例报告如下; 病例:欧××.男九岁,住院号141625 入院前两个月不慎被拖拉机辗伤腹部致肝破裂,在当地医院手术治疗。术中见肝左叶内后面、肝十二指肠韧带处一2Cm裂伤深达1.5Cm 左叶膈面被膜剥脱,面积约5×6Cm。予肝修补。于术后第10天和15天曾两次出现上腹部痛疼、呕血、便血、每次出血量约两大碗,保守治疗好转。于82年6月22日转我院。查体:一般状况尚可,贫血貌巩膜皮肤无黄染全身皮肤无出血点。心、肺无异常,腹部膨隆,腹壁静脉无曲张,左上腹部肋缘下可见横形手术瘢痕,全腹软,剑突下压痛( ),反跳痛(-),肝肋下1.5Cm,质地柔软,无触痛,脾未     

反义miR-21通过RECK抑制胶质瘤细胞侵袭性生长的体内外研究

目的 探讨胶质瘤细胞LN229中RECK作为miR-21的调控靶点,在胶质瘤侵袭性生长中的作用.方法 将反义miR-21(AS-miR-21)寡核苷酸转染至人脑胶质瘤细胞LN229中.Real-time PCR检测LN229细胞中miR-21的表达量.荧光素酶实验检测miR-21对RECK的调控关系.Transwell实验评价LN229细胞侵袭能力的变化,应用Western blot检测细胞内MMP2/9和RECK蛋白水平的变化,ELISA实验检测培养基中活性MMP2/9的表达量,动物实验评价体内条件下肿瘤侵袭性的变化.结果 Real-time PCR显示转染组中miR-21的表达量与对照组相比下调60%.荧光素酶实验证明RECK是miR-21的靶点.Transwell实验证实胶质瘤细胞侵袭能力下降,Western blot和ELISA实验证实MMP2/9表达降低,动物实验及免疫荧光反映肿瘤侵袭性生长受抑制.结论 反义miR-21通过上调RECK的表达而抑制恶性胶质瘤细胞的侵袭性生长.

Abstract：

Objective To investigate the regulation of miR - 21 on invasion growth of human glioma cells by RECK.Method The human glioma LN229 cells were transfected with AS - miR - 21 or scrambled sequences by Lipofectamine2000.Real time PCR was conducted to detect the expression of miR-21.Luciferase experiment was performed to detect the relationship between miR-21 and RECK.The expression of RECK was evaluated by Western blot.The invasion ability was evaluated by transwell assay and subcutaneous models.Western Blot, ELISA and immunofluorescence were used to estimate the changes of MMP2/9.Results The expression of miR - 21 in LN229 cells decreased after transfection with AS-miR-21. It was proved that RECK was a direct target of miR -21 by luciferase experiment.Meanwhile, the high expression of RECK protein in AS - miR -21 group conformed its important function in this mechanism.Transwell assay demonstrated decreased invasion capability of LN229 cell lines transfected with AS- miR- 21.Western blot, ELISA, and immunofluorescence demonstrated the levels of MMP2/9 were down -regulated in AS -miR -21 group compared with control and scrambled group.Conclusions AS - miR -21 could depress the invasion of glioma cells owing to up - regulating the level of RECK which could inhibit MMP2/9 activities both in vitro and vivo.     

敲低miR-19a、miR-19b抑制人脑胶质瘤细胞生长的体外研究

目的 探究敲低microRNA(miR)-19a和miR-19b表达对人脑胶质瘤细胞系SNB19细胞生物学特征的影响.方法 常规培养SNB19细胞,脂质体介导miR-19a、miR-19b抑制物转染SNB19细胞,同时设未转染组(对照组)、无义序列转染组和miR-19a+miR-19b抑制物转染组.应用RT-PCR检测转染后细胞miR-19a、miR-19b的表达,MTT、流式细胞术分别检测转染后细胞增殖能力和细胞周期的变化,Transwell实验检测转染后细胞的侵袭能力.结果 与对照组和无义序列转染组比较,抑制物转染组细胞miR-19a和miR-19b的表达、增殖和侵袭能力均降低,差异有统计学意义(P＜0.05),而且miR-19a+miR-19b抑制物转染组细胞miR-19a和miR-19b的表达、转染后2、3、4、5 d细胞的增殖能力、侵袭能力均低于miR-19a、miR-19b抑制物转染组,差异有统计学意义(P＜0.05);抑制物转染组较对照组和无义序列转染组细胞周期延迟,且miR-19a+miR-19b抑制物转染组较miR-19a、miR-19b抑制物单独转染组细胞延迟更为明显.结论 miR-19a和miR-19b可能是癌微RNA,有望作为人脑胶质瘤基因治疗的侯选靶点.

Abstract：

objective To investigate the effects of knocking down of miR-19a and miR-19b on the biological characteristics of SNB19 glioblastoma cells. Methods Oligonucleotides inhibitor of miR-19a and miR-19b (miR-19a inhibitor or miR-19b inhibitor) mediated by lipofectamine2000 were transfected to SNB19 cells to knock down miR-19a and miR-19b; control group (without transfection),group D (performing transfection with nonsense sequence) and group E (performing transfection with both miR-19a inhibitor and miR-19b inhibitor) were established. Real time PCR was conducted to detect the expressions ofmiR-19a and miR-19b in these groups after the transfection. The cell proliferation rate and cell cycle kinetics were detected by 3-(4, 5-Dime- -thylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and flow cytometry, respectively; the cell invasive ability was evaluated by Transwell assay.Results As compared with those in control group and group D, the expressions of miR-19a and miR-19b, proliferation activity and invasive ability of cells in the miR-19a/19b inhibitor transfected cells (group A/B) were significantly reduced (P＜0.05). The expressions of miR-19a and miR-19b and the proliferation activity and invasive ability of cells 2, 3, 4 and 5 d after the transfection in group E were significantly reduced as compared with those in group A/B (P＜0.05). Delayed cell cycle in group A/B and group E was noted as compared with that in control group and group D; and group E enjoyed more obviously delayed eell cycle than group A/B (P＜0.05). Conclusion MiR-19a and miR-19b might be oncomiRs, and may be candidate target miRNAs for gene therapy of glioma.     

Ad-PTEN增强LY294002对人胶质瘤细胞系U251的生长抑制作用

目的在体外实验中,利用携带野生型PTEN基因的重组腺病毒（Ad—PTEN）和P13K的小分子抑制剂LY294002联合抑制P13K／AKT信号通路,观察两者联合对人胶质瘤细胞系U251生长有无正向协同作用及探讨产生这种作用的机制。方法15251细胞按处理方式不同分为4组：DMSO对照组、空载病毒对照组、LY294002组和联合组（LY294002＋Ad—PTEN组）。在U251细胞系中导入LY294002并转染Ad—PTEN病毒后,提取总蛋白,用Westernblotting检测PTEN表达状态及P13K、AKT表达情况;用MTT法检测Ad—PTEN和LY294002对U251生长抑制率、流式细胞仪检测细胞周期、AnnexinV法检测细胞凋亡,观察导人LY294002和转染Ad—PTEN后U251增殖能力的改变;用划痕实验、Transwell实验观察LY294002及Ad—PTEN对U251迁移和侵袭能力的影响;Westernblotting检测P13K／AKT信号通路下游相关蛋白表达水平变化。结果Westernblotting显示：与DMSO组和空载病毒组相比,LY294002组P13K、AKT蛋白表达水平明显降低,联合组（LY294002＋Ad—PTEN）的WEN蛋白明显上调,而P13K、AKT蛋白下降水平比LY294002组更为显著。联合组与LY294002组、空载病毒组、DMSO组相比细胞周期阻滞在G0／G1期;联合组凋亡率比其他三组明显增加;自培养第2天起,联合组细胞增殖速率呈下降趋势。联合组侵袭和迁移细胞的相对数少于LY294002组、空载病毒组和DMSO组。Westernblotting结果证明位于P13K／AKT下游的MMP-2、MMP-9、PCNA、Bcl-2、CyclinD1、NF—KB、FAK蛋白表达水平显著下调。结论小分子抑制剂LY294002能够有效抑制U251中P13K、AKT蛋白的表达,联合转染Ad—PTEN后不仅能提高PTEN蛋白的表达水平,对P13K、AKT的抑制更为显著。在体外实验中,联合Ad—PTEN和LY294002能够有效地通过阻滞细胞周期、减少细胞凋亡来抑制U251的增殖能力,并能够抑制细胞的侵袭和迁移能力,两者联合使用具有正向协同作用。联合Ad—PTEN和LY294002对U251的生长与侵袭的抑制作用与P13K／AKT下游的MMP-2、MMP-9、PCNA、Bcl-2、CyclinD1、NF—KB、FAK蛋白表达水平下调有关。联合腺病毒转染技术和小分子抑制剂调控P13K／AKT信号通路是治疗胶质瘤的新途径。     

肥胖病因之饮食及其相关因素的临床调研与分析

运用临床调研的方法,对肥胖者的饮食习惯及其相关因素进行调查分析,结果表明三种不良的饮食习惯与肥胖关系最密切,提示减少动物性食物、高热量食物的摄入,规范进餐,干预饮食习惯,可有效预防肥胖症的形成。     

"补肝"涵义辨析

虚则补之,补以治虚。补是治疗虚证的大法,但是在五脏治法之中,唯补肝法其涵义多端,不可不辨。1　畅其用曰补疏畅条达肝气为补,这是补肝最早的涵义。肝主疏泄,性喜条达而恶抑郁,其气畅达则气机和调,抑遏则为病。能使肝气条达舒畅则无病。疏畅肝气中用味辛之品,如《素问·脏气法时论》曰:“肝欲散,急食辛以散之,用辛补之,酸泻之”。首先提出了辛味补肝。对此,张介宾释曰:“木不宜郁,故欲以辛散之,顺其性为补,逆其性者为泻,肝喜散而恶收,故辛为补,酸为泻之味”。所以说畅其用为补。此处的补泻是对脏腑生理功能活动本身之喜恶而言。因此,畅其用…