经颅多普勒超声和CT在腔隙性脑梗死中的应用价值

目的探讨经颅多普勒(TCD)与CT在腔隙性脑梗死中的诊断价值.方法对210例腔隙性脑梗死患者行TCD与CT检测,并进行对比分析.结果CT首次确诊为腔隙性脑梗死149例(70.9%),而TCD检测结果193例异常,异常率达91.9%,异常率明显高于CT.CT显示72.38%的病例发生在基底节区域,TCD则显示病灶区的脑血流动力学异常,表现为动脉供血不足、脑血管痉挛、血管狭窄、脑动脉硬化症.结论同时对患者行TCD和CT检测,对腔隙性脑梗死患者的临床诊断、病因和危险因素的追查可提供依据.     

消化道穿孔超声检查临床应用的评价

目的：为了评价普通超声在诊断消化道穿孔中的价值。方法：１１９例经手术证实的消化道穿孔病人，术前行Ｘ线检查，６８例术前行普通超声检查。将所有的检查结果进行回顾性评价及对比分析。结果：行Ｘ线检查的１１９例病人，９１例为阳性，符合率为７５％（９１／１１９），假阴性２８例，占２５％（２８／１１９）。行超声检查的６８例中阳性者占７８％（５５／６８）。其中１０例超声发现膈下气体而Ｘ线为阴性。结论：普通超声在诊断消化道穿孔中可弥补Ｘ线的不足，在急腹症的鉴别诊断中具有重要地位。     

Utility of the Guy’s Stone Score in predicting different aspects of percutaneous nephrolithotomy

Objective

To evaluate Guy’s scoring system (GSS) as a grading system for complexity of kidney stone before percutaneous nephrolithotomy (PCNL) as a predictor for different items of outcome.

人转化生长因子β3的构建

目的:获取人转化生长因子β₃的编码基因,开发抗衰老的皮肤药物。 方法：采用重叠PCR的方法,设计4对引物,进行4次PCR合成人转化生长因子β₃的编码基 因。 结果：4次PCR后,经2％的琼脂糖凝胶电泳可见一357 bp的条带,将电泳产物回收,连接入pMD-18T载体,经测序分析证实,所获得DNA片段为人转化生长因子β₃的编码基因。 结论：利用重叠PCR方法能够成功构建人转化生长因子β₃的编码基因。     

Crystallization/Melting Kinetics and Morphological Analysis of Polyphenylene Sulfide

Crystallization and melting kinetics of polyphenylene sulfide (PPS) are determined using fast scanning calorimetry. The temperature dependence of half‐time crystallization of isothermally melt‐crystallized PPS shows a downward convex curve with a minimum at 160 °C. The minimum crystallization half‐time is about 3 s. The analysis of heating rate dependence of the melting temperature reveals a zero‐entropy‐production melting temperature of the sample. The microstructure of the sample, which is prepared by fast scanning calorimetry, is investigated by small‐angle X‐ray scattering and polarizing optical microscopy. The crystallinity and lamellar thickness of the sample annealed for 400 s decrease with decreasing crystallization temperature. The size of spherulites becomes small as the isothermal temperature is decreased. Transcrystalline morphology is observed near the surface between the sample and nitrogen gas at a crystallization temperature of 120 °C. The thickness of the transcrystalline layer disappears as the isothermal temperature is increased.     

Three‐dimensional structures of bacteriophage neck subunits are shared in Podoviridae,Siphoviridae and Myoviridae

Tailed bacteriophages (Caudovirales) are divided into three families: Myoviridae with long contractile tails, Siphoviridae with long noncontractile tails and Podoviridae with short noncontractile tails. All have an icosahedral head with a portal vertex connected to a neck structure followed by a tail. Bacteriophage Mu belongs to the Myoviridae family. Herein, the gp29 portal subunit and neck subunits gp35, gp36 and gp37 of the Mu phage were purified to elucidate their arrangement in the neck. Both gp29 and gp36 were monomeric in solution, like the corresponding subunits of Podoviridae P22 and Siphoviridae SPP1. X‐ray crystal structure of gp36 showed structural similarity to neck subunits of Siphoviridae and Podoviridae. The gp36 structure has a characteristic aromatic hydrophobic core, and the structure of the ring form of the Mu phage connector deduced from the Siphoviridae and Podoviridae connector showed that this feature builds the contact surface between gp36 subunits. Structural comparison with the neck of Siphoviridae and Podoviridae also implies direct interaction between gp36 and gp29. Because gp35 and gp36 form a stable complex, we predict that the head‐portal ring (gp29), the connector complex (gp36 and gp35), the tail terminator (gp37) and the tube (gp40) are arranged in the Mu phage neck in this order.     

Use of high resolution dual‐energy x‐ray absorptiometry‐region free analysis (DXA‐RFA) to detect local periprosthetic bone remodeling events

Dual‐energy x‐ray absorptiometry (DXA) is the gold standard method for measuring periprosthetic bone remodeling, but relies on a region of interest (ROI) analysis approach. While this addresses issues of anatomic variability, it is insensitive to bone remodeling events at the sub‐ROI level. We have validated a high‐spatial resolution tool, termed DXA‐region free analysis (DXA‐RFA) that uses advanced image processing approaches to allow quantitation of bone mineral density (BMD) at the individual pixel (data‐point) level. Here we compared the resolution of bone remodeling measurements made around a stemless femoral prosthesis in 18 subjects over 24 months using ROI‐based analysis versus that made using DXA‐RFA. Using the ROI approach the regional pattern of BMD change varied by region, with greatest loss in ROI5 (20%, p < 0.001), and largest gain in ROI4 (6%, p < 0.05). Analysis using DXA‐RFA showed a focal zone of increased BMD localized to the prosthesis–bone interface (30–40%, p < 0.001) that was not resolved using conventional DXA analysis. The 20% bone loss observed in ROI5 with conventional DXA was resolved to a focal area adjacent to the cut surface of the infero‐medial femoral neck (up to 40%, p < 0.0001). DXA‐RFA enables high resolution analysis of DXA datasets without the limitations incurred using ROI‐based approaches. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:712–716, 2015.     

螺旋CT扫描结合CA19-9检测在胰腺癌术前分期中的探讨

目的探讨螺旋CT双期扫描结合CAl9-9对判断胰腺癌可切除性方面的价值。方法回顾性收集37例在我院行螺旋CT双期扫描并经手术病理证实的患者，全部患者均有术前血清CAl9-9检测结果。结果CT对胰腺癌不可切除的判断准确率高达100％，对可切除性判断准确率只有78．57％。胰腺癌病人中CAl9-9的阳性率为82．86％，CAl9-9与肿瘤大小有关；CAl9—9在可切除组与不可切除组间存在显著性差异。结论螺旋CT结合CAl9-9在判定胰腺癌不可切除性方面有一定价值，尚不能得出CAl9-9对协助CT判定胰腺癌可切除性方面有肯定意义的结论。