IGF—Ⅰ和TGF—β1对幼年及成年兔关节软骨细胞增殖和代谢的作用

目的 观察IGF－I和TGF－β1对幼年及成年兔关节软骨细胞增殖和代谢的作用。方法 采用体外培养兔关节软骨细胞的方法。利用^3H-TdR和^35S-Na2SO4掺入分别反映软骨细胞增殖和骨质蛋白多糖的合成,应用TUNEL法检测软骨细胞的凋亡,结果 随年龄增加、关节软骨细胞凋亡增加,同时增殖和蛋白多糖的合成能力下降,而且对IGF－I和TGF－β1反应性亦呈年龄相关下降。但是IGF－I和TGF－β1均可能在一定程度上以剂量依赖方式促进软骨细胞^3H-TdR和^35S-Na2SO4掺入增加。结论 外源性TGF－I和TGF－β1在体外可促进不同年龄兔关节软骨细胞增殖和基质合成,提示外源性生长因子在骨关节炎的防治上可能有一定意义。     

骨关节炎滑膜中尿激酶型纤溶酶原激活物及其受体蛋白的表达

OBJECTIVE: To study the expression of urokinase-type plasminogen activator (uPA) and its receptor (uPAR) in the synovial tissures, and the significance of uPA and uPAR in degradation of extracellular matrices in osteoarthritis (OA), then analyse the possible relationship between uPA and uPAR. METHODS: Immunohistochemical analysis technique was used to detect uPA and uPAR protein expression and distribution in synovial tissues in 26 OA patients and 10 mormal individuals. RESULTS: Positive staining of uPA and uPAR protein were detected in 19 cases in the 26 OA samples (73%), while only 2 positive cases were seen in the 10 mormal tissues (20%). The expression rate of uPA protein was markedly higher in OA than in the normal (P < 0.01). The expression of uPAR was seen in 14 cases in all 26 OA samples (54.6%), while there was only one case in 10 normal samples (10%), the positive rate was significantly higher in OA than in normal samples (P < 0.05). Positive expression of uPA and uPAR proteins were found in synovial lining cells, mononuclear cells, macrophage-like cells and endothelial cells. Using the correction analysis, we found a positive correlation between uPA and uPAR reactivity in the synovial tissues in OA (r = 0.920, P < 0.01). CONCLUSIONS: The high expression of uPA and uPAR protein in OA synovial tissues indicate that the uPA system may play an important role in the process of synovitis and degradation of OA cartilage extracellular matrices. In the pathologic process of OA, uPA and uPAR coordinate each other, lead to the genesis and development of OA cartilage degradation.     

关节内注射透明质酸钠治疗膝关节骨关节炎

骨关节炎是一种慢性关节疾病,是由老年或其它各种原因引起的以关节软骨退变为主要病理特征的非炎症性疾患。临床上治疗除了理疗、药物对症、缓解症状及少数情况下手术治疗外,尚无彻底有效的治疗方法。近年来,氨基糖类物质对关节软骨的保护学说的提出使骨关节炎的治疗方案有了新的拓展。本文     

30例骨关节炎关节镜手术治疗体会

<正> 我院自1999年开始进行关节镜手术,至今共收治骨关节炎患者30例,经过精心治疗与护理,均获得满意效果,现报告下。 1.临床资料 本组共30例,其中男12例,女18例,年龄50—84岁,平均65岁,其中不痛不肿仅有不适感3例,轻度疼痛5例,中度疼痛8例,重度疼痛8例,剧烈疼痛6例。     

胫骨结节上楔形截骨治疗膝关节骨关节炎10例

膝关节骨关节炎是一种常见的老年性骨关节病,以膝关节始动性疼痛为初期症状,进而出现关节僵硬、活动受限、关节畸形,严重影响日常生活.对于疼痛较重且伴有膝内翻者,手术治疗可减轻痛苦,改善关节功能.1996～1998年,采用胫骨结节上楔形截骨治疗膝关节骨关节炎10例(12膝),收到满意的近期疗效.