Event-specific analytical methods for six genetically modified maize events using visual and real-time loop-mediated isothermal amplification

Currently 138 genetically modified (GM) maize events have been authorized for commercial cultivation, comprising more than 65 per cent stacked events. With the increase in number of GM maize events globally, cost- and time-efficient diagnostics with on-site applicability are required to check for authorized GM events. Six GM maize events, namely, Bt11, GA21, MON810, MON89034, NK603 and TC1507, also present in 89 stacked events, are being widely commercialized in more than 17 countries. Visual and real-time loop-mediated isothermal amplification (LAMP) assays targeting these six GM maize events are being reported in the present study. Specificity of the developed LAMP assays was confirmed using fourteen commercialized GM maize events. Limit of detection of visual and real-time LAMP assays targeting Bt11, GA21, MON810, MON89034 and TC1507, was up to 0.01%, detecting 8 target copies, and for NK603 event-specific assays, was up to 0.1% detecting 73 target copies. Practical applicability of developed LAMP assays was verified using a set of five stacked GM maize events, namely, Bt11 × GA21, MON89034 × NK603, MON89034 × NK603 × TC1507, TC1507 × NK603 and TC1507 × MON810; and six powdered maize samples of proficiency testing. The reported LAMP assays can be efficiently employed for screening for presence of selected GM maize events in single or stacked form.     

基于B／／s模式的智能用电服务系统设计与开发

针对智能电网下智能终端的应用,结合现有量测方法和计算机网络技术,提出了一种基于B／S模式的智能用电服务系统的实现方案。该系统以智能电表的应用为硬件基础,通过网络协议编程构建用电自动测量网络,并实现对用户用电数据的实时采集。软件则以2LAMP（Linux＋Apache＋MySQL＋PHP）为开发平台,综合利用AJAX（异步JavaScript和xML,AsynchronousJavaScriptandXML）以及实时数据库技术实现系统的整体设计。该用电智能服务系统以浏览器为用户终端,可以为用户提供综合耗电统计、在线用电付费、智能用电决策以及实时信息交流等便捷而多样化的服务入口,从而为实现用户智能用电提供有力支撑。     

基于LAMP的高性能计算用户组织架构管理系统设计与实现

针对现有高性能计算用户组织架构管理系统面临的用户体验差、网络开销大和存取效率低等关键问题,提出了一种基于LAMP的高性能计算用户组织架构管理系统的实现方法。该方法采用B/S架构,Twig与HTML相结合的方式减轻了服务端的负担,改善了用户体验;采用REST框架与Cache机制对海量临时数据进行缓存,降低了开发难度和网络开销;采用树形结构对分层数据进行存取,提高了数据存取效率,且可扩展性好。     

基于CentOS 5.4环境构建LAMP平台

本文简单介绍了CentOS和LAMP,详细阐述了基于CentOS5．4系统环境的LAMP平台构建。     

基于LAMP的MVC模式的研究与应用

LAMP以其高效、快捷、安全和成本低廉等优势已成为当前中小型企业应用开发的首先工具,并取得了巨大成功.然而在大型企业应用中却由于担心服务器负载和开发成本维护等原因而变得小心翼翼,为此提出了一种采用与MVC模式结合,并使用Ajax和缓存技术来支持大型企业开发与应用的方法.     

基于Linux流媒体的开放教学、实践平台的搭建

随着网络技术的发展,高效的教学实践平台对高校教育影响巨大.建立基于Linux流媒体的开放教学、实践平台对共享教学资源、学生自主学习基于流媒体的教学内容,学习网站管理和Web数据库编程,提高实际的编程能力都有实际意义.通过该平台学生可以在课后与教师交流、答疑,并获得预习和复习的指导.本文阐述了建立普通高校规范、统一、开放的教学实践平台的思想,并且讲述了如何利用开源软件Linux、LAMP、vsFtP和real Server架设基于Linux流媒体的开放教学、实践平台.     

教学互动网站的实现

文章主要介绍了LAMP环境下基于开源软件建设教学互动网站的实现过程,内容包括系统的硬件软件环境、LAMP环境的构建和配置、学习内容管理系统的配置、博客系统的配置、维基系统的配置和社交网络服务的配置等。     

环介导等温扩增技术在转基因成分检测中的应用

随着全球转基因作物商业化种植面积持续增长,转基因成分检测作为转基因作物安全管理的重要技术支撑受到越来越多的关注,世界各国与地区不断致力于转基因成分检测技术的开发。环介导等温扩增（loop-mediatedisothermal amplification,LAMP）技术由于简单快速等特点,近年来在转基因检测领域备受青睐。本文对LAMP技术在转基因成分检测中的最新研究与应用及其发展前景加以综述。     

基于Web的高校毕业生就业信息服务平台设计

针对毕业生、用人企业和就业办管理人员三方角色在高校就业工作中的实际需要,采用B/S模式,以LAMP作为开发环境,使用jQuery、Ajax等技术实现前端界面交互,应用Zend Framework进行后台MVC分层编程,设计开发了一个基于Web的高校毕业生就业信息服务平台,方便了毕业生、用人企业与学校三者之间的在线沟通。     

Point-of-Care Diagnostic Platforms for Loop-Mediated Isothermal Amplification

The loop-mediated isothermal amplification (LAMP) method is one of the Nucleic acid amplification tests (NAATs) that allows for the amplification of target regions without using a thermal cycle. With its unique primer design, LAMP ensures the rapid replication of the targeted DNA region with high specificity and high efficiency. LAMP technology is used for diagnostic purposes in pathogen detection due to its ease of use, low cost, and simplicity without requiring complex equipment. A wide range of LAMP diagnostic platforms have been developed for applications in bacteria, virus, and parasitic pathogen detection. Herein, the methodology of LAMP technology and its applications in pathogen detection and SNP genotyping and mutation detection are discussed. Point-of-care (PoC) LAMP platforms designed with the principles of microfluidic chip technology, including LAMP-on-a-chip, paper-based LAMP, and smartphone-based LAMP applications have been elaborated. LAMP technology represents a fast, robust, and reliable diagnostic platform for point-of-care testing.