15N-甘氨酸示踪试验观察小肠全部切除患儿肠道吸收功能

食物进入人体后 ,经胃肠道消化吸收其中各种营养成分。由于某些病变导致肠部分切除或大部切除后 ,必然会发生营养吸收障碍。我们收治１例８岁男孩 ,因患肠梗阻小肠坏死 ,行小肠全切除术 ,术后营养必须靠静脉供应。为了探讨患儿十二指肠及剩余结肠是否还有吸收食物营养的功能 ,我们采用１５Ｎ＿甘氨酸示踪试验了解患儿消化道的吸收功能。１．１仪器和材料使用Ｈｐ５８９０ＭＳＥｎｇｉｎｅ气相色谱 -质谱联用仪 (ＧＭ＿ＭＳ)。试剂为 １５Ｎ＿甘氨酸 ,丰度９６ % ,上海化工研究院产品 ;三氟醋酐 ,德国Ｍｅｒｃｈ＿Ｓｃｈｕｃｈａｒａｔ公司产…     

A new method for early diagnosis of carcinoma in bile duct-sonography of common bile duct before and after injection of ceosunin.

42 cases of dilatation (7-15 mm) of extrahepatic bile duct after ceosunin injection (cholagogic method) were observed ultrasonographically. Among the 18 cases of testified obstructive lesions of the bile duct, 13 (72%) showed widening of the bile duct with an increase of calibre from 9.8 mm to 11.7 mm, 3 (16%) had no obvious change, and 2 (12%) shrank. Among the 24 cases of non-obstruction 22 (92%) shrank with a decrease calibre from 9.6 mm to 6.3 mm, and 2 (8%) showed no obvious change. After receiving cholagogue, 9 cases of periampullary carcinoma in the obstructive group were confronted with widening of the bile duct calibre. After longitudinal and transverse rotation scanning method was used, 8 of the 9 cases had their tumor mass visualized. Combined cholagogic and rotation scanning methods raised the lesion manifestation rate of lower bile duct from 41% to 88%. A dilated bile duct unable to show shrinkage of calibre after cholagogue injection is believed to be a criterion for judging the presence of     

机械通气治疗呼吸衰竭成败因素分析

近年机械通气治疗呼吸衰竭(呼衰)已得到更多重视和应用，为了积累经验、发现不足、减少失误，进一步提高治疗成功率，我们对23例呼衰机械通气治疗的疗效与成败因素进行分析。     

急性有机磷农药中毒及并发症的救治

<正>有机磷农药是我国目前使用最广、使用量最大的杀虫剂,大多为磷酸酯类或硫代磷酸酯类化合物,对人畜的毒性主要是对乙酰胆碱酯酶的抑制,引起乙酰胆碱蓄积,使胆碱能神经受到持续冲动,导致先兴奋后衰竭的一系列的毒蕈碱样、烟碱样和中枢神经系统等症状;严重患者可因昏迷和呼吸衰竭而死亡。     

辐射诱发雄性小鼠各阶段生殖细胞染色体畸变的研究

<正> 我们采用受精卵技术,观察了2.1Gy X射线照射后的雄性小鼠不同发育阶段生殖细胞染色体的变化,结果报告如下。 1 材料与方法 选用我校动物部提供的8周龄,体重20g以上的昆明种小白鼠。用深部X射线治疗机对雄鼠1次全身均匀照射2.OGy(剂量率为0.44Gy/min)。与经     

氟化钙对软化牙本质的再矿化作用

深龋时，脱矿总是先于细菌侵入而发生，无菌的脱矿牙本质经适当的材料垫底充填之后，有可能再矿化，恢复其原有功能、本文采用氯化钙（CaF2）作垫底材料，与常规氢氧化钙和氧化锌进行对照使用，结果表明，氰化钙有较强的再矿化作用。现报告如下。材料和方法1．氰化钙来源：我院药局按北京医科大学口腔医学院口腔儿童牙病科的采集方法[1]，即于25℃的条件下，将CaCe2他合液与NaF他合液混合，得白色沉淀，经过过滤烘干，得CaF2粉剂。2．分组：我们将深图病人随机分三组各10例。第一组采用CaF2垫底法，用蒸馏水调CaF2。第二组采用Ca（OH…     

Transcutaneous ultrasound of the cervical esophagus in patients with esophageal carcinoma.

Transcutaneous ultrasound of the cervical esophagus was performed in 46 patients with esophageal carcinoma and in 35 controls. The former had 24 upper segmental lesions and 22 lower segmental lesions. The level of the sternoclavicular joint was used to divide the esophagus into the upper segmental (USE) and lower segmental esophagus (LSE). The anterior esophageal wall thickness and luminal dimensions were measured before and immediately after phonation. The mean wall thickness in the controls was 1.8 mm before phonation and 2.1 mm after phonation, with a significant difference (t test,P<0.05). The mean wall thickness in the USE carcinoma group was 4.3 mm and 4.4 mm before and after phonation respectively. There was a significant difference between the controis and USE carcinoma groups (t test, P<0.05). The cross sectional area, which was calculated as the product of anteroposterior and lateral diameters, averaged 28 mm~2 before phonation in the controls and increased to 44 mm~2 after phonation (t test, P     

腹腔镜治疗老年萎缩性胆囊炎44例临床分析

目的:探讨老年萎缩性胆囊炎患者的腹腔镜治疗方法,并预防胆管损伤并发症的发生。方法:回顾分析腹腔镜治疗老年萎缩性胆囊炎44例患者的临床资料。结果:41例顺利完成手术,3例中转开腹,12例放置腹腔引流管,术后肝下积液1例,无其他并发症发生及死亡病例。结论:老年人萎缩性胆囊炎的腹腔镜治疗属于复杂手术,与术中操作技巧,把握好中转手术的时机,充分围手术期准备,熟练术中操作技巧,把握中转开腹的时机,合理使用器械等是预防和减少并发症的关键。     

平板运动试验在诊断冠状动脉疾病中的应用

目的评价平板运动试验(TET)在冠状动脉病变(CAD)诊断中的应用价值. 方法以冠状动脉造影术(CAG)检测结果作为金标准,统计分析TET对正常冠脉组及CAD患者共204例的临床检测价值.TET采用NCVC亚方案,评价指标包括最大运动负荷量(MET)、ST段改变、SBP变化. 结果TET中,ST段改变、运动后3min SBP比值(3′)和MET是CAD检测的有效指标,ST段改变与运动后3min SBP比值(3′)的综合评估可提高TET对CAD的临床检测价值,其诊断准确性、敏感性和特异性分别达78.4%、70.4%和91.1%. 结论TET是CAD比较有效的临床检测手段.     

胞嘧啶脱氨酶基因修饰神经干细胞及其表达的离体实验研究

目的 探讨胞嘧啶脱氨酶(cytimidine deaminase，CD)基因修饰神经干细胞及其基因表达。方法 通过构建真核表达质粒pCMVCD，限制性内切酶消化鉴定后，采用Lipofectamine 2000脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stem cells，NSCs)，G418筛选阳性克隆，加入不同浓度的5-氟胞嘧啶(5-Flourocytosine，5-FC)，MTT比色法测定NSCs的生存率。结果 本实验成功地培养并鉴定了神经干细胞，并将CD基因成功地转染了神经干细胞，G418阳性NSCs对低浓度5-FC高度敏感。结论 CD基因修饰神经干细胞的离体实验研究为干细胞治疗研究提供依据。