肝阳上亢型高血压患者心率变异低频指标与血压变异指标的关系

目的:了解肝阳上亢型高血压患者的心率变异低频指标与血压变异指标的关系,探讨自主神经功能变化在肝阳上亢型高血压病中的作用。方法:对40例肝阳上亢型高血压住院患者的临床治疗资料(主要为血压变异指标资料及心率变异指标资料)进行回顾性分析。结果:不同年龄,性别患者的血压变异各有不同。心率变异低频指标与血压变异指标的相关系数为0.351～0.790,P<0.01。结论:肝阳上亢型高血压患者的心率变异低频指标与血压变异指标呈中度相关。     

长期视远剥夺对人眼集合近点和调节近点的影响

Objective To study effects of sustained deprivation of far sight on human near point of convergence (NPC) and near point of accommodation (NPA). Methods Eight subjects were divided into two groups by age (group A ≥30 years old; group B<30 years old). All subjects had worked in the enclosed laboratory, unable to see the outside for 75 days. NPC and NPA were measured every 15 days. Results NPC values of both groups all increased when time elapsed. NPC of group A measured in the 75th day and NPC of group B in the 30th day all had statistical differences, when compared with the base NPC values measured in the 15th day (P<0.05). And NPC value of group B measured in the 45th, 60th and 75th days increased markedly, when compared with that of the 15th day (P<0.01 . However, statistical analysis showed that no significant difference could be seen in NPA values of the two groups (P>0.05). Conclusions Sustained deprivation of far sight could induce an increase in NPC values to various degreeal, but effects on NPA were not obvious, as noted in our experiment.     

长期视远剥夺对人眼集合近点和调节近点的影响

Objective To study effects of sustained deprivation of far sight on human near point of convergence (NPC) and near point of accommodation (NPA). Methods Eight subjects were divided into two groups by age (group A ≥30 years old; group B<30 years old). All subjects had worked in the enclosed laboratory, unable to see the outside for 75 days. NPC and NPA were measured every 15 days. Results NPC values of both groups all increased when time elapsed. NPC of group A measured in the 75th day and NPC of group B in the 30th day all had statistical differences, when compared with the base NPC values measured in the 15th day (P<0.05). And NPC value of group B measured in the 45th, 60th and 75th days increased markedly, when compared with that of the 15th day (P<0.01 . However, statistical analysis showed that no significant difference could be seen in NPA values of the two groups (P>0.05). Conclusions Sustained deprivation of far sight could induce an increase in NPC values to various degreeal, but effects on NPA were not obvious, as noted in our experiment.     

长期视远剥夺对人眼集合近点和调节近点的影响

Objective To study effects of sustained deprivation of far sight on human near point of convergence (NPC) and near point of accommodation (NPA). Methods Eight subjects were divided into two groups by age (group A ≥30 years old; group B<30 years old). All subjects had worked in the enclosed laboratory, unable to see the outside for 75 days. NPC and NPA were measured every 15 days. Results NPC values of both groups all increased when time elapsed. NPC of group A measured in the 75th day and NPC of group B in the 30th day all had statistical differences, when compared with the base NPC values measured in the 15th day (P<0.05). And NPC value of group B measured in the 45th, 60th and 75th days increased markedly, when compared with that of the 15th day (P<0.01 . However, statistical analysis showed that no significant difference could be seen in NPA values of the two groups (P>0.05). Conclusions Sustained deprivation of far sight could induce an increase in NPC values to various degreeal, but effects on NPA were not obvious, as noted in our experiment.     

预防及抢先治疗下异基因干细胞移植后巨细胞病毒感染现状分析

目的 了解预防及抢先治疗下异基因干细胞移植后巨细胞病毒(CMV)感染现状,为改进治疗措施、提高治疗效果提供依据.方法 统计进行异基因干细胞移植且针对CMV进行预防、监测及抢先治疗的患者310例,分析CMV感染特点.结果 异基因干细胞移植310例,发生CMV感染81例,感染率26.1%.其中CMV血症78例,发生率24.3%(78/310).CMV病3例(视网膜炎2例,CMV脑炎1例),发生率1%(3/310).CMV感染组移植物抗宿主病发生率高.未发现严重药物不良反应.治疗后,78例CMV转阴,占96.3%(78/81),但其中16例转阴后再次出现阳性,占19.8%(16/81).3例持续阳性(其中2例死亡,1例放弃后死亡),占3.7%(3/81).反复或持续阳性者监测淋巴细胞亚群,均显示NK细胞升高,CD4+T细胞降低,CD8+T细胞升高.结论 预防及抢先治疗是异基因干细胞移植后CMV感染的有效方法,在此治疗方案下病毒复发率仍高,需定期监测,部分持续阳性需寻找新的方法.     

心肌缺氧状态下间充质干细胞对巨噬细胞 M2 极化的影响及其机制研究

目的 探讨心肌缺氧状态下间充质干细胞（MSC）对巨噬细胞极化的影响,并揭示其调控巨噬 细胞极化的机制。方法 在M0 型巨噬细胞培养液中加入无糖无氧培养的心肌细胞上清液,与MSC 共培养。 采用流式细胞术、Western blotting 及逆转录聚合酶链反应检测M1/M2 型巨噬细胞生物学标志,以判断MSC 在模拟心肌缺氧状态下对巨噬细胞极化的影响,并观察MSC 对巨噬细胞髓样分化因子88 及其上游TLR2、 TLR4 受体表达的影响,以及其下游的细胞核转录因子-κB（NF-κB）和丝裂原活化蛋白激酶（MAPK） 信号通路磷酸化的水平。结果 与M0 组比较,模拟心肌缺氧状态下培养的M0 巨噬细胞成功诱导M1 极化 （P <0.05）;与MSC 共培养促进M1 向M2 极化（P <0.05）。同等条件下加入不同浓度髓样分化因子88 抑制剂后, 与M0+OGD 组比较,M2 型巨噬细胞比例随着ST2825 剂量的增加逐渐升高（P <0.05）。与M0+OGD 组比较, M0+OGD+MSC 组白细胞介素10、转化生成因子-β1 表达水平升高（P <0.05）。Western blotting 检测显示 MSC 共培养后,巨噬细胞TLR2、TLR4 及髓样分化因子88 表达下调,其下游的NF-κB 和MAPK 信号通 路标志分子P65、IKKAα/β、JNK1/2、P38、ERK1/2 等磷酸化水平降低。结论 在心肌缺氧状态下,MSC 促进巨噬细胞M2 极化。其机制之一可能是MSC 下调巨噬细胞TLR2、TLR4 及髓样分化因子88 表达,抑制 TLR2/4- 髓样分化因子88 信号及其下游的NF-κB 和MAPK 通路。     

调脂中药的实验研究探讨

调脂中药实验研究内涵主要体现在调节血脂代谢、抗脂质氧化损伤、改善血管内皮功能、调节微量元素（锌、铜、镁）、影响低密度脂蛋白受体基因表达等方面。本研究根据抗高脂血症中药实验研究资料,分类探讨调脂中药的实验研究内涵。     

微波在临床治疗上的应用进展

微波是指频率在300 MHz～300 GHz,在空气中波长为1 m～1 mm的电磁波.微波治疗是常用的超高频治疗方法之一,已被广泛应用于临床.目前常用的是915 MHz与2 450 MHz微波,其中以2 450 MHz微波最为常用.现对微波在临床治疗上的应用作简单综述.     

脂汰清对实验性高脂血症兔降血脂机制研究

目的：探讨脂汰清对实验性高脂血症兔降血脂的作用。方法：选择普通级新西兰家兔 60 只,雌雄不限,随机挑选 7 只作正常对照组,普通饲料饲养;其余家兔制作高脂血症模型,高脂饲粒饲养1 个月后,将总胆固醇（TC）水平过高和过低的家兔11只除外,剩余 42 只家兔随机平均分为模型组,脂汰清高、中、低剂量组,辛伐他汀组,山楂精降脂组,每组 7 只,改喂普通饲料。正常组和模型组动物以成人药量脂汰清胶囊溶于10 m L 蒸馏水注射;辛伐他汀组以3 倍成人药量的辛伐他汀 ＋ 阿拉伯树胶溶于 10 m L 蒸馏水中注射;山楂精降脂组以 3 倍成人药量山楂精降脂片 ＋ 阿拉伯树胶溶于 10 m L 蒸馏水中注射;脂汰清高、中、低剂量组分别以成人量 6 倍（0.72 g·kg^-1）、3 倍（0.36 g· kg^-1）、1 倍（0.12 g kg 1）＋ 阿拉伯树胶溶于 10 m L 蒸馏水注射。用酶法及免疫透射比浊法检测血清 TC、三酰甘油（TG）、高密度脂蛋白（H DL）、低密度脂蛋白（LDL）。严格按试剂盒工作。结果：与治疗前比较,各给药组 TC、TG、H DL 明显改善,差异有统计学意义（P 〈 0.05）,与辛伐他汀组、山楂精降脂组相比,脂汰清各组各指标改善明显,差异有统计学意义（P 〈 0.05）。各组治疗前后 LDL 变化不大,差异无统计学意义（P 〉 0.05）。结论：脂汰清对高脂血症兔具有明显的降血脂作用。     

脂汰清颗粒对高脂血症兔血清锌、铜、镁的影响

目的 探讨脂汰清颗粒对高脂血症兔血清微量元素锌(Zn)、酮(Cu)、镁(Mg)的影响.方法 将1.5 kg～2.0kg普通级雄性新西兰兔随机选取10只作空白对照组,喂普通饲料.余兔按高脂饲料配方1饲喂6周,制成高脂血症模型,再随机分为模型组、脂汰清治疗组、辛伐他汀对照组、山楂精降脂片时照组.从第7周开始,正常组喂全价营养饲料,余组改喂高脂饲料配方2.同时,脂汰清组及两对照组分别给相应溶化药物,空白组和模型组给等量生理盐水.灌胃连续11周后取血清检测微量元素Zn、Cu、Mg.结果 模型组与空白组比较,Zn、Cu、Mg均呈降低趋势(P>0.05).脂汰清治疗组与模型组比较,Zn、Cu、Mg含量轻度上升,且Cu、Mg值超过空白组,但无统计学意义(P>0.05).山楂精降脂片对照组Mg显著升高,与模型组、空白组和辛伐他汀对照组比较,均有统计学意义(P<0.05).结论 脂汰清颗粒对血清Zn、Cu、Mg元素的调节作用不明显.