Ca2+ mobilization in physiologically stimulated single T cells gradually increases with peptide concentration (analog signaling)

We have investigated Ca²⁺ mobilization in single T cells stimulated with their physiological ligand, i.e. antigenic peptide bound to major histocompatibility complex (MHC) molecules on antigen-presenting cells (APC). Fibroblasts expressing I-E^d class II molecules were pulsed with a peptide derived from the λ2³¹⁵ immunoglobulin light chain. Onto such antigen-pulsed fibroblasts were sedimented cloned Th1 cells loaded with Fura-2. Changes in cytosolic Ca²⁺ concentration in single T cells were continually monitored by use of an imaging system based on fluorometry. Ca²⁺ mobilization was both peptide-specific and MHC-restricted. Within seconds of the initial APC-T cell contact, a Ca²⁺ spike could be observed. The Ca²⁺ response gradually declined over a 25-min period, during which oscillations were noted. Various parameters characterizing the magnitude of the Ca²⁺ response (latency, increase rate, max and mean Ca²⁺ increase, frequency and period of oscillations) all correlated with the amount of peptide used for pulsing the fibroblasts. Thus, Ca²⁺ mobilization in single T cells appears not to be an all or none phenomenon. Rather, activation is incremental (analog signaling), the degree of Ca²⁺ mobilization probably being related to the number of stimulatory peptide-MHC complexes on the surface of the APC. The extent of calcium mobilization and lymphokine production (interleukin (IL)-2, IL-3, interferon-γ) correlated, at least at the population level.     

左心室造影功能成像的临床应用

为探讨左心室造影功能成像的价值与限度，我们选择冠脉及左心室造影者38例行左室功能成像，观察左室壁运动情况并与节段法室壁运动定量分析比较。结果示左室收缩正常18例，其各节段EF值平均39％，节段性收缩减弱7例，无收缩3例，矛盾收缩4例，普遍性收缩减弱6例，病变节段的EF值平均5～19％，明显低于正常者（P＜0．01）。结论：左室功能成像效果直观且客观，可作为室壁运动定量分析的一个重要补充。     

Pixel value modification using RVG-4 automatic exposure compensation for instant high-contrast images

The RVG-4 permits automatic exposure compensation (AEC). The purpose of this investigation was to determine the effects of AEC on image contrast. Images were made either with or without a dental QA jaw phantom using a fixed image projection geometry. Exposures were 6.3 through 27.3 μC/kg using an X-ray generator operated at 70 kVp. Region of interest pixel value distributions were measured at tissue thicknesses in this phantom, and the average pixel values and signal-to-noise ratios (SNR) were calculated. The use of AEC without an object in place resulted in a disproportionate relationship between pixel value and exposure with a marked reduction in SNR. The use of AEC on under- and over-exposed images of the phantom simultaneously enhanced image contrast and reduced SNR. Thus, AEC provides a convenient and quick method for achieving high-contrast images with sub-optimal exposures, however, this could lead to inappropriate patient dosages if the function is used for over-exposed images. AEC reduces the SNR and produces disproportionate pixel values relative to exposure.     

First experimental study of carbon dioxide digital subtraction lymphangiography

Fourteen pigs with an average weight of 17 kg were used in this study. Under general anesthesia and magnification 1–3 ccm/kg of carbon dioxide were administered in the lymph vessels of the front and rear legs. Imaging of the peripheral lymph vessels, lymph nodes and the thoracic duct was achieved with digital subtraction angiography. The quality of lymphangiography was satisfactory and comparable with that of the standard non-ionic contrast agent. It is anticipated that further technical evolution will permit the application of CO₂/DSA lymphangiography to man. Carbon dioxide is non-nephrotoxic and is non-allergic; it is inexpensive, can be administered in unlimited quantity and is quickly eliminated via the pulmonary system.     

农村居民医疗服务潜在需求量及影响因素分析

目的 了解农村居民医疗服务的潜在需求及其影响因素。方法 采用分层多阶段整群抽样方法，对湖北省宜昌地区5县1839名农村居民进行问卷调查。结果 该地区治病未就诊率为13．21％，男女性未就诊率分别为12．01％，15．56％，采用Logistic回归分析方法，筛选出影响患病未就治的主要因素为性别、健康状况、对健康的重视程度、婚姻状况以及不同县等。结论 在农村要完善合作医疗保障制度，对医疗机构建设应以提高服务质量、降低成本、遏制费用增长为主要目的，并能提供更多的医疗服务，使患能及时利用医疗服务。     

代谢综合征患者肾动脉狭窄的SCTA与DSA对比研究

目的探讨代谢综合征(MS)患者中肾动脉狭窄的发生率及无创性筛检的必要性与可靠性。方法45例MS患者预行肾动脉螺旋CT血管造影(SCTA),对所检出的14例肾动脉狭窄者行肾动脉数字减影血管造影(DSA)对照,并对SCTA及DSA结果进行对比分析。结果45例MS患者中SCTA显示肾动脉正常31例,其余14例患者存在单侧或双侧肾动脉狭窄,DSA对照显示肾动脉正常6条,肾动脉狭窄22条,与SCTA显示的情况基本符合。结论MS患者中肾动脉狭窄的发生率约31.1%,对可疑患者应常规予以肾动脉SCTA检查。     

涉外护理专业学生社区毕业实习效果评价

目的 探讨涉外护理专业学生社区毕业实习模式。方法 随机设定实验组(36人),参加慢性疾病调查、建立健康档案和进行相应的健康教育,结束时与不参加社区毕业实习的对照Ⅰ组(31人)、对照Ⅱ组(38人)的实习生同时接受基础知识和能力闭卷测试。然后统计调查问卷和健康档案的正确率,并将三组测试成绩进行比较。结果 实验组调查问卷和健康档案的正确率≥95％。实习生基础知识得分实验组(43.14±2.78)、对照Ⅱ组(42.53±1.96)与对照Ⅰ组(40.65±2.47)比较;能力得分实验组(47.00±2.34)与对照Ⅰ组(41.61±3.41)、对照Ⅱ组(41.42±3.52)比较;总分实验组(90.14±4.30)与对照Ⅰ组(82.26±5.33)、对照Ⅱ组(83.95±4.13)比较,均P<0.01,差异有极显著性意义。结论 社区毕业实习强化了实习生社区护理的基本知识,培养了实习生社区护理的相关能力。但有待于规范化。     

"优质服务达标创先"活动体会

为探讨优质为兵服务，促进医院全面建设，本阐述了“开展优质服务达标创先”活动的意义，指明该活动的重点是：加强医德医风建设；坚持依法治医；加强内涵建设，确保合理医疗。提出现阶段应着力抓好以下几个方面工作：坚决纠正乱收费；坚决纠正收取“红包”，礼品,坚决纠正服务作风粗疏。     

Induced abortion in China and the advances of post abortion family planning service

<正>This is a review of current situation of induced abortion and post abortion family planning service in China. Induced abortion is an important issue in reproductive health. This article reviewed the distribution of induced abortion in various time, areas, and population in China, and explored the character, reason, and harm to reproductive health of induced abortion. Furthermore, this article introduces the concept of Quality of Care Program in Family Planning, and discusses how important and necessary it is to introduce Quality of Care Program in Family Planning to China.