中心静脉导管胸腔内灌注恩格菲治疗癌性胸水30例

目的：观察应用中心静脉导管胸腔内灌注恩格菲在癌性胸水治疗中的疗效。方法：时30例癌性胸水患者行胸腔内置中心静脉导管，最大限度地排尽积液后，胸腔内进行恩格菲12-20mL灌注治疗，待引流液消失维持48h以上后拔除中心静脉导管，4周后复查。结果：全组总有效率为87％，所有患者未出现严重的全身性毒副反应结论：恩格菲胸腔内灌注治疗癌性胸水安全、有效。[著者文摘]     

Expression and significance of HIF-1α and ET-1 in gastrointestinal stromal tumors

Objective To explore the expression and the value of HIF-1α,and ET-1 in judging the prognosis of gastrointestinal stromal tumors (GISTs). Methods The expression of HIF-1α, and ET-1 protein was examined in 76 GISTs by immunohistochemistry S -P methods. Results There was a positive correlation between the expression of HIF-1 α and ET-1 ( P < 0.05 ). The positive expression rate of HIF-1 α and ET-1 was 73.68% (50/76) ,and 65.79% (50/76) respectively,which was related with histologicial grade, tumor diameter, infiltration and metastasis, nuclear division rating of GISTs ( P < 0.05 ), but had no relationship with patients' age, gender, initial position of the tumor ( P > 0.05 ). There was statistically sig-nificant difference in the expression of HIF-1 α and ET-1 in the following groups:among the three classes of very low-risk and low-risk, middle-risk, high-risk, bewteen the diameter < 2 cm and > 5 cm (P < 0.05). The more malignant degree and larger diameter, the more highly positive expression rate ( P < 0.05 ). The positive expression in the groups with infiltration and metastasis, and nuclear division ≥5/50 HP was sig-nificantly higher than the groups without infiltration and metastasis, and nuclear division < 5/50 HP (P < 0.05). Conclusion The expression of HIF-1α had a significant correlation with ET-1. HIF-1α,and ET-1 expression was closely related with the prognosis of GISTs,and can serve as important predictors for survival.     

芹菜素抗肿瘤作用分子机制的研究进展

近年来对膳食和流行病学调查研究结果表明,水果和蔬菜的摄入对机体具有明显的健康促进作用,且可预防多种类型肿瘤的发生,因此从膳食中获取有效预防和治疗肿瘤的成份已成为目前人们普遍关注的问题。芹菜素（apigenin）又称芹黄素,是一种广泛存在于植物中的黄酮类化合物。有研究表明,芹菜素具有抗炎、抗氧化、抗肿瘤、抗病毒、免疫调节、保护心血管、抗突变等生物学作用。本文综述了芹菜素抗肿瘤作用分子机制的研究进展。     

抑瘤素M在炎症性疾病中的研究进展

抑瘤素M(OSM)是一种属于IL-6家族的多功能细胞因子,它主要由免疫细胞产生,参与了肿瘤、炎症、造血、骨骼组织改变、肝脏再生以及神经系统、心血管和代谢疾病等多种重要的生理及病理过程。OSM在炎症性疾病中发挥了重要作用。OSM生物学活性既有促炎作用,又有抑炎作用,但在不同的疾病以及同一疾病不同时间可能表现出不同的生物学功能。文章就OSM在炎症性疾病中的作用作综述。     

慢病毒介导的RNAi沉默TLR4基因对人乙肝病毒相关肝细胞癌裸鼠移植瘤生长的影响北大核心CSCD

目的探讨TLR4基因RNA干扰(RNAi)的重组慢病毒载体对人乙肝病毒相关肝细胞癌裸鼠移植瘤TLR4基因表达和移植瘤生长的影响。方法构建4个miR-TLR4质粒和一个阴性对照质粒,选择干扰效果最明显的质粒进行慢病毒包装。建立人乙肝病毒相关肝细胞癌裸鼠移植瘤模型,随机分成实验组、阴性对照组和空白对照组。隔天1次向各组动物肿瘤内分别注射TLR4 miRNA慢病毒颗粒、携带无关序列的慢病毒颗粒或0.2 ml 0.9%氯化钠溶液,共5次,测量各组肿瘤体积的大小,绘制肿瘤生长曲线;11天后处死裸鼠,应用半定量反转录-聚合酶链反应(RT-PCR)和Western blot技术检测移植瘤中TLR4基因的表达。结果与空白对照组和阴性对照组相比,实验组的移植瘤生长速度明显减慢(P<0.05),并且实验组移植瘤细胞TLR4 mRNA和蛋白表达明显下降(P<0.05)。结论 TLR4 miRNA慢病毒载体瘤内注射可抑制人乙肝病毒相关肝细胞癌裸鼠移植瘤的生长。     

Expression and significance of HIF-1α and ET-1 in gastrointestinal stromal tumors

Objective To explore the expression and the value of HIF-1α,and ET-1 in judging the prognosis of gastrointestinal stromal tumors (GISTs). Methods The expression of HIF-1α, and ET-1 protein was examined in 76 GISTs by immunohistochemistry S -P methods. Results There was a positive correlation between the expression of HIF-1 α and ET-1 ( P < 0.05 ). The positive expression rate of HIF-1 α and ET-1 was 73.68% (50/76) ,and 65.79% (50/76) respectively,which was related with histologicial grade, tumor diameter, infiltration and metastasis, nuclear division rating of GISTs ( P < 0.05 ), but had no relationship with patients' age, gender, initial position of the tumor ( P > 0.05 ). There was statistically sig-nificant difference in the expression of HIF-1 α and ET-1 in the following groups:among the three classes of very low-risk and low-risk, middle-risk, high-risk, bewteen the diameter < 2 cm and > 5 cm (P < 0.05). The more malignant degree and larger diameter, the more highly positive expression rate ( P < 0.05 ). The positive expression in the groups with infiltration and metastasis, and nuclear division ≥5/50 HP was sig-nificantly higher than the groups without infiltration and metastasis, and nuclear division < 5/50 HP (P < 0.05). Conclusion The expression of HIF-1α had a significant correlation with ET-1. HIF-1α,and ET-1 expression was closely related with the prognosis of GISTs,and can serve as important predictors for survival.     

全身化疗联合顺铂腹腔灌注治疗中晚期消化道肿瘤30例疗效观察

本科自 1998年以来 ,采用全身化疗联合腹腔灌注顺铂治疗 30例中晚期消化道恶性肿瘤患者 ,取得了良好的效果 ,现报告如下 :1　资料与方法一般资料 :本组 30例 ,男 2 1例 ,女 9例。平均年龄 5 1岁 (30～ 6 8岁 )。病程 1个月～ 3年。胃癌 14例 ,右半结肠癌 4例 ,左半结肠癌 2例 ,直肠癌 8例 ,胰腺癌 2例。全部病例术后均经病理检查或腹水染色体等检查确诊。腺癌 2 0例 ,粘液癌 2例 ,低分化腺癌 8例。 30例患者中不能手术者2例 ,姑息性切除术者 3例 ,术后出现复发者 4例 ,腹膜腔转移者 2例。所有病例按UICC国际分期标准均为Ⅱ期以上。治疗方…