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61.
Planar patch-clamp force microscopy on living cells   总被引:2,自引:0,他引:2  
Here we report a new combination of the patch-clamp technique with the atomic force microscope (AFM). A planar patch-clamp chip microstructured from borosilicate glass was used as a support for mechanical probing of living cells. The setup not only allows for immobilizing even a non-adherent cell for measurements of its mechanical properties, but also for simultaneously measuring the electrophysiological properties of a single cell. As a proof of principle experiment we measured the voltage-induced membrane movement of HEK293 and Jurkat cells in the whole-cell voltage clamp configuration. The results of these measurements are in good agreement with previous studies. By using the planar patch-clamp chip for immobilization, the AFM not only can image non-adhering cells, but also gets easily access to an electrophysiologically controlled cellular probe at low vibrational noise.  相似文献   
62.
This paper presents an artificial emotional-cognitive system-based autonomous robot control architecture for a four-wheel driven and four-wheel steered mobile robot. Discrete stochastic state-space mathematical model is considered for behavioral and emotional transition processes of the autonomous mobile robot in the dynamic realistic environment. The term of cognitive mechanism system which is composed from rule base and reinforcement self-learning algorithm explain all of the deliberative events such as learning, reasoning and memory (rule spaces) of the autonomous mobile robot. The artificial cognitive model of autonomous robot control architecture has a dynamic associative memory including behavioral transition rules which are able to be learned for achieving multi-objective robot tasks. Motivation module of architecture has been considered as behavioral gain effect generator for achieving multi-objective robot tasks. According to emotional and behavioral state transition probabilities, artificial emotions determine sequences of behaviors for long-term action planning. Also reinforcement self-learning and reasoning ability of artificial cognitive model and motivational gain effects of proposed architecture can be observed on the executing behavioral sequences during simulation. The posture and speed of the robot and the configurations, speeds and torques of the wheels and all deliberative and cognitive events can be observed from the simulation plant and virtual reality viewer. This study constitutes basis for the multi-goal robot tasks and artificial emotions and cognitive mechanism-based behavior generation experiments on a real mobile robot.  相似文献   
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64.
Almost all magnetic resonance electrical impedance tomography (MREIT) reconstruction algorithms proposed to date assume isotropic conductivity in order to simplify the image reconstruction. However, it is well known that most of biological tissues have anisotropic conductivity values. In this study, four novel anisotropic conductivity reconstruction algorithms are proposed to reconstruct high resolution conductivity tensor images. Performances of these four algorithms and a previously proposed algorithm are evaluated in several aspects and compared.  相似文献   
65.
This article aims to demonstrate the effects of using different boundary conditions and different computational volume dimensions in numerical simulations of periodic metamaterial arrays. A double band metamaterial unit cell design will be utilized to show that use of different boundary conditions may result in simulation of dissimilar periodic array topologies with completely different electromagnetic responses. It will also be shown that dimensions of the computational volume may strongly affect the overall response of the metamaterial structure due to varying electromagnetic coupling between the array elements. © 2013 Wiley Periodicals, Inc. Int J RF and Microwave CAE, 2013.  相似文献   
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67.
This paper investigates the organizational and locational features of supply networks in the car industry. Its primary theoretical concern is to distinguish mainstream theoretical approaches to this topic and critically discuss them in the context of peripheral economies. The objective of this paper, therefore, is to shed light on the Turkish car industry and focus on the local supply chain of Tofas, one of the most important carmakers in Turkey.  相似文献   
68.
In this paper, we address the medium access control (MAC) problem in ad-hoc networks from the energy-efficiency perspective and develop a residual-energy-based collision resolution algorithm (CRA) for energy-limited terminals. In this interval-splitting-based algorithm, packets involved in a collision are partitioned into subsets according to the amount of residual battery energy left at the corresponding terminals, and retransmissions are scheduled according to a tree structure. To avoid possible performance degradations for cases of not evenly spread battery energies, we propose a hybrid approach that interchangeably uses energy-based and first-come-first-served CRA’s to resolve packet conflicts. We extend the proposed energy-based collision resolution (CR) approach to cases without hard energy constraints but, rather, with energy-efficiency objectives. The algorithm then utilizes the distance from the receiver as the criterion. We then address energy-efficient conflict resolution in general multi-hop ad-hoc networks. In this context, a useful but yet simple method is proposed to reduce the interdependence between collision resolution processes at different receivers, which would otherwise distort the general structure of tree-splitting algorithms. We evaluate the proposed algorithms via simulation for communication systems ranging from simple single-cell classical collision channel models to general multi-hop wireless ad-hoc networks.  相似文献   
69.
Polycrystalline ZnS semiconducting films have been prepared by ultrasonic spray pyrolysis technique in the form of planar and sandwich configurations using amorphous and ZnO coated glass substrates. Deposition of ZnS films by the spray pyrolysis has been studied extensively by several investigators and the process parameters have been optimized to obtain films with good characteristics. However we report for the first time the preparation of ZnS films sandwiched between top and bottom electrodes on the transparent conducting ZnO films that have been produced by the spray pyrolysis technique. The produced ZnS films have been crystallized in a wurtzite structure. The electrical properties of the samples having planar and sandwich structures have been measured in dark at room temperature by applying the voltage values between 0.01 and 100 V.  相似文献   
70.
Ballistic response of single or multi-layered metal armor systems subjected to high velocity impact loads was investigated in many experimental, theoretical and numerical studies. In this study, influences of plasma spray surface coating on high velocity impact resistance of AA 6061 T651 aluminum plates were analyzed experimentally. Two different types of surface coating were applied to plates using plasma spray. Using 9.00 mm Parabellum bullets, ballistic performance of both uncoated and coated plates was tested. After the impact tests, penetration depth including plate bending on the front face and bulging on the rear face of the target plate was measured. The improvement on the ballistic resistance of the coated plates was clearly observed. The increase in non-perforating projectile velocity and the decrease in penetration depth were both experienced.  相似文献   
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