肥大细胞的浸润对胃癌生长转移的影响

OBJECTIVE: To investigate the relationship between mast cell infiltration and the development and metastasis of gastric carcinoma. METHODS: Alcian blue-Safranin O staining and image analysis system were employed to observe, classify and quantify the mast cells in 74 gastric carcinoma specimens. RESULTS: Mast cells resided predominantly in the marginal area of the tumor and most of them were mature type, active and capable of discharging granules into the cytoplasm. The mast cells in the marginal area of the tumor were significantly more numerous than those in the matrix of the tumor (P<0.01) and there was a positive correlation between them (r=0.303, P<0.01). The density of the mast cells was inversely correlated to the depth of tumor invasion and the number of lymph node metastasis. CONCLUSIONS: Mast cell infiltration in the matrix of the tumor plays an important role in preventing the invasion and metastasis of gastric carcinoma possibly by release of heparin and other bioactive substances. Mast cell quantity in the marginal area of the tumor may serve as an indicator for prognostic assessment of gastric carcinoma.     

广东汉族人群乙肝疫苗免疫应答水平与HLA—DRB1＊02，07，09的相关性

OBJECTIVE: To study the association between immune response to hepatitis B vaccine and HLA-DRB1*02, 07, 09 genes in the population of Han nationality in Guangdong Province. METHODS: A total of 1 400 healthy college students of Han nationality from Guangdong Province were recruited in this study, who received standard courses of vaccination with recombinant hepatitis B virus vaccine at month 0, 1 and 6. Eight weeks after the final vaccination, the serum levels of anti-HBs antibody was examined with enzyme-linked immunosorbent assay (ELISA). Detection of HLA-DRB1*02, 07, 09 alleles were performed with PCR using sequence-specific primers in 118 individuals who failed to produce or produced insufficient antibodies (nonresponders or weak responders), 100 normal responders and 45 strong responders. RESULTS: The frequency of HLA-DRB1*07 in nonresponders or weak responders was significantly higher than that in normal or strong responders (15.25% vs 3.44%, P<0.05), while the frequency of HLA-DRB1*02 was much lower (5.08% vs 17.9%, P<0.05). The frequency of HLA-DRB1*09 showed no obvious difference between the 2 groups (19.49% vs 17.9%, P>0.05). CONCLUSION: Failure or deficiency in the response to HBV vaccine is associated with the HLA-DRB1*07 gene, while HLA-DRB1*02 gene is related to the normal vaccination process or overreaction. Definite relation of HLA-DRB1*09 to the response to HBV vaccine is not found.     

2,6—二叔丁基对甲酚对大鼠尿生化指标的影响

以２０，６０和１８０ｍｇ／ｋｇ体重剂量给大鼠喂食２，６－二叔丁基对甲酚（ＢＨＴ），检测尿中ＡＳＴ、ＡＬＰ、ＬＤＨ和ＧＧＴ等指标变化。结果表明：随染毒时间延长，各尿酶含量逐渐出现显著性增高，病理学检查发现高剂量组有１例间质性肾炎，提示ＢＨＴ对肾功能可能产生有害作用。     

尼卡地平控制性降压对家犬脊髓血流的影响

目的　观察尼卡地平控制性降压对家犬脊髓血流的影响。方法　成年杂种犬 6只 ,体重 12 5～ 16kg ,以 2 5 %硫喷妥钠麻醉。股动脉置管监测MAP。以尼卡地平 8μg·kg 1·min 1持续静脉注射控制性降压。以激光多普勒血流仪测定脊髓血流 (SCBF)。结果　降压前MAP为 (12 5 7±10 6 )mmHg ,降压后为 (72 0± 11 2 )mmHg ,平均下降 4 2 8%。降压前SCBF为 (9 80± 1 0 5 )v ,降压后为 (8 0 4± 0 96 )v ,降低幅度为 18%。结论　尼卡地平控制性降压对SCBF影响较小 ,可安全用于脊髓手术。     

抑郁症的睡眠脑电图与人脑兴奋守恒假说

目的　探索抑郁症状与睡眠脑电图参数的相关性。方法　对 18例抑郁症和 10例正常人评定汉密尔顿抑郁评定量表 (HAMD)和检测睡眠脑电图。结果　HAMD总分与觉醒次数及REM密度分别呈显著正相关 (r分别等于 0 .4 0 8和 0 .4 4 5 ,P均 <0 .0 5 )。结论　抑郁症和正常人的白天中枢抑制与夜间中枢兴奋相关 ,从而支持人脑兴奋守恒假说。     

60岁以上老人植物性神经功能特点研究

本文以综合指标测定法和心搏间距变化为指标测定了71名60岁以上老人的植物性神经功能,结果表明:植物性神经系统功能在总体上呈现交感神经功能增强,同时控制心血管活动的植物性神经功能也发生显著的异常。     

脊神经节神经元周围突至肾及体壁的分支投射

实验将快蓝(FB)和核黄(NY)分别注入大鼠左肾纤维膜下及左体壁神经干内,结果在荧光镜下发现左T_(9-13),L1脊神经后根节(DRG)内存在双标记细胞,提示大鼠T_(9-13),L1段DRG内的部分神经元的周围突分支支配肾及体壁,为肾绞痛所致牵涉痛的解释,提供了神经解剖学基础。     

端粒酶调节相关基因TRAP与肿瘤生物学特性的关系

目的：研究TRAP基因在人肿瘤组织中的表达及其与人端粒酶逆转录酶(hTERT)的作用相关性，探讨其在人肿瘤发生中的作用。方法：通过原核表达TRAP蛋白免疫新西兰白兔，制备特异性多克隆抗体；免疫组化检测TRAP及hTERT在人肿瘤组织中的表达；通过构建TRAP真核表达载体、基因转染，观察TRAP对细胞hTERT转录、端粒酶活性和癌细胞生长及成瘤性的影响。结果：通过大肠杆菌表达的TRAP蛋白免疫、制备多克隆抗体，经Westem blot鉴定与TRAP具有特异结合性；免疫组化显示247例恶性肿瘤中有213例TRAP阳性，表达率为86．2％，而在所有癌旁组织中TRAP为阴性。另外，交界性及癌前病变与良性病变组织中TRAP表达差异也有显著性：进一步检测卵巢生发上皮肿瘤、乳腺癌、肝癌及中枢神经系统肿瘤组织hTERT表达，显示hTERT与TRAP表达的结果相一致。TRAP正义转染使hTERT及端粒酶活性升高，细胞增殖加快；而反义转染时，两者均下降，细胞增殖受抑。裸鼠成瘤性实验表明：正义TRAP使癌细胞成瘤性增强，而反义则抑制移植瘤的生长。结论：TRAP表达存在于人癌组织及部分癌前病变，与癌细胞恶性表型相关，并与hTERT表达一致。TRAP基因参与对端粒酶的调节作用，可能与人肿瘤发生有关。     

Missense mutations and evolutionary conserved amino acids at the human hypoxanthine phosphoribosyl-transferase locus.

Molecular characterization of in vivo mutation at the human hypoxanthine phosphoribosyltransferase (hprt) locus has revealed a broad spectrum of mutation, both with regard to germ-line mutation in Lesch-Nyhan and gout patients, and somatic mutation in 6-thioguanine resistant T-lymphocytes from healthy individuals. The pattern of missense mutation shows a non-random distribution with a preferential location to codons for amino acids which are identical in human and the two parasites Schistosoma mansoni and Plasmodium falciparum. Although these 'evolutionary conserved' amino acids account for only 32% of the amino acids in the human hprt protein, they are involved in 76% of the missense mutations at the hprt locus in human T-lymphocytes, 67% in Lesch-Nyhan patients (with severe hprt-deficiency), but only 43% in gout patients (with partial hprt deficiency). This observation supports the notion that evolutionary conserved amino acids constitute functionally important sites in the hprt enzyme, and missense mutations affecting these amino acids will often lead to complete loss of enzyme activity. Substitutions of 'non-conserved' amino acids cause less severe hprt-deficiency (as seen in the gout patients), or may even escape clinical diagnosis. These considerations are important for the understanding of structure-activity relationships in the hprt protein, possible differences between hprt mutational spectra in germ-line and somatic cells, and the mutational spectra induced by specific exogeneous mutagens.