针刺镇痛中α和β受体的作用及其对脑内5—羟色胺代谢的影响

<正> 据报道,针刺镇痛时脑内去甲肾上腺素(NE)的含量明显降低;损毁 NE 能神经元胞体密集分布的蓝斑或上行途径,或者用双硫醒等抑制 NE 合成以降低脑内 NE 含量,均可增强针刺镇痛;刺激蓝斑或注射 NE 前体二羟基苯丝氨酸使脑内 NE 含量增加时,可对抗针刺     

Effect of Runing Ⅱon the Growth and Metastasis of Transplanted Tumor in Mammary Cancer-bearing Mice and Its Mechanism

objective;To study the effect of Runing Ⅱ(a Chinese herbal preparation for mammary cancer)on the growth and metastasis of transplanted tumor of mammary cancer MA-891-bearing TA2 mice and its mechanism.Methods;The model of mammary cancer MA.891 cell strain transplanted tumor of TA2 mice with lung metastasis were developed to observe the effect of Runing Ⅱon the growth and metastasis of the transplanted tumor.The immunohistochemical method and image analysis were adopted to detect the levels of vascular endothelial growth factor(VEGF),vascular endothelial growth factor receptor(VEGFR),and micro-vessel count(MVC)and micro-vessel area(MVA).Results;In the Runing Ⅱgroup,the tumor weight inhibition rate and the lung metastasis inhibition rate were 37.3%and 65.4%respectively,the tumor growth and lung metastasis were obviously inhibited;And the levels of VEGF and VEGFR,MVC and MVA were significantly decreased as compared with those in the tumor-bearing control group(P＜0.05).Conclusion;The Chinese herbal preparation Running Ⅱcan inhibit the metastasis of tumor through inhibiting the angiogenesis,and the mechanism is possibly related with down-regulation of VEGF and VEGFR expression.     

Regulative Effects of Auricular Acupuncture,Moxibustion and Chinese Herbs on Immunologic Function in the D-Galactose-induced Aging Mouse

Objective： To observe the regulative effect of auricular acupuncture, moxibustion and Chinese herbs on immunologic function in the D-galactose-induced aging mouse and to probe the feasibility and possible mechanisms for delaying aging, so as to provide experimental basis for acupuncture, moxibustion and Chinese herbs for delaying aging. Methods： Aging mouse model was established by subcutaneous injection of D-galactose, and treated by auricular acupuncture, auricular acupuncture plus moxibustion, and auricular acupuncture plus Chinese herbs respectively for 6 weeks and then serum interleukin-2 （IL-2） and IL-6 contents and splenic lymphocyte transformation rate were compared among the groups. Results： Compared with the young group, the serum IL-2 level and the splenic lymphocyte transformation rate significantly decreased and the IL-6 level significantly increased in the aging mouse. After treatment by auricular acupuncture, auricular acupuncture plus Chinese herbs, especially auricular acupuncture plus moxibustion, the serum IL-2 level and the splenic lymphocyte transformation rate significantly increased, and the IL-6 level significantly decreased as compared with the model group. Conclusions： Combined application of auricular acupuncture, moxibustion and Chinese herbs can improve the decline or dysfunction of immunological function in the aging organism to a certain extent.     

Effects of Moxibustion Pretreating on SOD and MDA in the Rat of Global Brain Ischemia

Objective： To probe into the mechanism of moxibustion preconditioning in preventive brain-protecting effect. Methods： The global brain ischemia rat model was developed by blocking 4 arteries. Seventy-eight Wistar male rats were randomly divided into 5 groups： a normal control group, a sham-operation group, a brain ischemia group, a brain ischemia preconditioning group, a moxibustion pretreating group. The brains in the 5 groups were taken at 24 h, 48 h, and 72 h after operation respectively. Superoxide dismulase （SOD） activity was determined with xanthine oxidase method and malondialdehyde （MDA） content with thiobarbituric acid method. Results： After the operation, in the moxibustion preconditioning group, SOD activity significantly increased, especially 24 h after moxibustion preconditioning; and MDA content decreased, with a very significant difference as compared with that of the cerebral ischemia group （P〈0.01）. Conclusion： Moxibustion preconditioning protects the ischemic and anoxic brain tissue by increasing the activity of endogenous antioxidase.     

Experimental Study on Acupuncture Activating the Gonadotropin-releasing Hormone Neurons in Hypothalamus

Objective:To probe into the most effective site,extra-ordinary point,acupoint and channel for regulating reproductive endocrine function by means of the study on acupuncture activating the gonadotropin-releasing hormone neurons(GnRH) in hypothalamus.Methods:Female SD rats of reproductive age were used,and the in vivo study on GnRH neurons in hypothalamus was made with mimic sexual stimulation and feedback regulation.The neuron-activating effects of the acupoints on the channels pertaining to the zang-and fu...     

季节因素对男性尿睾酮、促间质细胞素水平的影响

现代研究表明 ,人体许多疾病的发生、发展均受生理节律的影响 ,许多治疗措施也会因人体生理功能的节律性变化而表现出时间性差异[1] ,所以 ,了解人体生理节律对于临床诊断、治疗疾病具有重要的意义。本实验拟通过对 2 0名健康成年男性四季尿睾酮、促间质细胞素 (ＬＨ)水平的动态观察 ,了解男性生殖内分泌的四季变化规律 ,为某些男性生殖内分泌疾病的时间治疗及因时保健提供参考。材料与方法一、材料1.尿样采集　分别于春 (3月 )、夏 (6月 )、秋 (9月 )、冬 (12月 )四季采集固定的 2 0名健康成年男性(2 0～ 2 3岁 )晨尿 10ｍｌ,- 70℃冰箱保…