广西地区壮汉族代谢综合征的患病率调查

目的分析广西地区代谢综合征（MS）及其不同组分的患病率并比较壮汉族的区别。方法采用分层随机整群抽样的方法，于2003至2005年在广西有代表性的地区开展糖尿病横断面调查。取其中有完整资料、年龄15岁以上的27240人列入本次MS分析。MS的诊断标准分别采用2005年国际糖尿病联盟（IDF）标准和中华医学会糖尿病学分会（CDS）于2004年提出的标准。结果（1）按IDF标准，MS总的粗患病率为13．15％，其中男性为12．41％，女性为14．11％；按2000年全国普查人口计算标化患病率为7．66％，其中男性为7．26％，女性为8．81％。按照CDS标准，MS总的粗患病率为10．75％，男13．45％，女7．28％；总标化患病率5．9％，男性7．21％，女性4．31％。MS总的患病率、男性和女性的患病率均随年龄增大而升高（P〈0．01）。（2）按IDF标准，壮族MS粗患病率和标化率分别为9．91％和6．69％；汉族MS粗患病率和标化率分别为13．71％和7．99％。按CDS标准，壮族MS粗患病率和标化率分别为6．92％和4．48％，汉族MS粗患病率和标化率分别为11．32％和6．14％。MS总标化患病率均为汉族高于壮族（P〈0．01）。（3）在肥胖、高血压、高甘油三酯（TG）、低高密度脂蛋白胆固醇（HDL-C）和高血糖五种代谢组分异常中，按IDF标准，总的粗患病率最高为高血压32．26％，按CDS标准则为高TG28．39％；在27240名调查对象中，65．82％（IDF标准）和56．13％（CDS标准）调查对象至少有一种代谢组分异常。结论广西MS粗患病率按IDF标准和CDS标准分别为13．15％和10．75％，按2000年全国人口标化患病率则分别为7．66％和5．90％。汉族MS患病率高于壮族。超过半数的调查对象至少有一项代谢组分异常。     

抗甲状腺药物致粒细胞缺乏症21例护理体会

抗甲状腺药物 (ATD)治疗甲状腺功能亢进症 (甲亢 )时 ,有可能出现严重的副作用———粒细胞缺乏症 (粒缺 )。中性粒细胞绝对值完全缺乏或低于 0 5× 1 0 9/L ,称为粒缺 ,其发生率虽然很低 ,在 0 5 %以下〔1〕,但病情凶险 ,如不及时处理 ,可导致死亡。我科 1 985年 8月至 2     

结缔组织生长因子影响体外培养瘢痕成纤维细胞ERK/MAPK信号通路活化及增殖的研究

Objective To explore the signal mechanism of proliferation stimulating effect of connec-tive tissue growth factor (CTGF) on hypertrophic scar (HS) derived fibroblasts. Methods <'3>H-TdR in-corporation technique was used to determine the proliferative effect of CTGF at different concentration. Western blot was applied to semi-quantitively analyze the expression of phosphorylated and total ERK1/2 protein after 0, 5, 10, 15, 30, and 60 min of CTGF stimulation, and the relative value of which was de-fined as AI to measure the activation of ERK1/2 signal pathway. PD98059 was admitted to specifically block the ERK1/2 pathway, and subsequently cell proliferation stimulated by CTGF was studied by MTT. Results CTGF could stimulate fibroblasts proliferation with a dose-dependant manner, and activa-ted the ERK1/2 signal pathway, and AI built up to 0.209±0.0201, reaching the apex at 15 min after stimulation performed. Inhibition of ERK1/2 activation by PD98059 suppressed CTGF-mediated HS fi-broblasts proliferation significantly, while OD significantly dropped. Conclusion CTGF induces a prolif-erative response in HS fibroblasts, and this action is mainly dependent on the activation of ERK1/2 signal pathway.     

三羟基异黄酮对增生性瘢痕成纤维细胞增殖与胶原合成作用的影响

目的探讨5,7,4′-三羟基异黄酮对体外培养的人增生性瘢痕成纤维细胞增殖及胶原合成作用的影响。方法以不同浓度三羟基异黄酮处理体外培养的人增生性瘢痕成纤维细胞分为25、50、100μmol/L三组,以加DMSO溶剂为对照组,MTT法测定成纤维细胞的增殖,3H-脯氨酸掺入法测定细胞胶原合成情况,逆转录聚合酶链式反应（RT-PCR）检测Ⅰ、Ⅲ型前胶原mRNA的表达。结果三羟基异黄酮能有效抑制增生性瘢痕成纤维细胞的增殖及胶原合成（P〈0.05）,且具有剂量-效应关系;100μmol/L浓度组作用后,与对照组相比,差异有显著意义（P〈0.01）;三羟基异黄酮作用后成纤维细胞Ⅰ、Ⅲ型前胶原mRNA的表达显著下调（P〈0.05）。结论三羟基异黄酮能抑制增生性瘢痕成纤维细胞的增殖与活性,可能成为治疗瘢痕及纤维化疾病的有效药物。     

积雪草甙预防隆乳术后包膜挛缩的细胞及分子机制初探

目的 初步探讨积雪草甙抑制隆乳术后包膜挛缩的细胞及分子机制.方法 将人体来源挛缩包膜组织进行体外分离培养,获得成纤维细胞,将含不同浓度积雪草甙的条件培养基作用于细胞后,采用3H-胸腺嘧啶核苷掺人法、3H-脯氨酸掺入法、Western-blot法分别检测细胞增殖、胶原合成及α-SMA蛋白表达的变化,结果采用SPSS11.0统计学软件分析,并进行t检验.结果 当积雪草甙浓度达到50 mg/L即对细胞DNA合成及胶原合成产生显著的抑制,抑制率分别为34.7%和30.1%,与空白组比较差异具有统计学意义(P＜0.05),随着药物浓度的增加,抑制作用逐渐增强;积雪草甙浓度达到25 mg/L时,α-SMA蛋白表达显著受到抑制,蛋白活化指数为1.673,与空白组比较差异具有统计学意义(P＜0.05).抑制作用与药物浓度正相关.结论 积雪草甙能有效抑制包膜来源成纤维细胞的增殖、胶原合成及向肌成纤维细胞的转分化.

Abstract：

Objective To explore the cellular and molecular mechanism of the inhibitory effect of asciaticoside on capsular contracture following breast augmentation. Methods Contractured capsule derived fibroblasts were cultured in medium with different concentration of asciaticoside. The cell proliferation, collage synthesis and α-SMA expression were detected by means of 3H-thymidine incorporation, 3H-proline incorporation, and Western-blot. The results were analyzed by SPSS 11.0 with t test. Results DNA and collagen synthesis of fibroblasts were dramatically inhibited when the asciaticoside reached the concentration of 50 mg/L. The inhibitory rate was 34.7% and 30.1%respectively, showing a significant difference from that in control group( P＜0.05 ). The inhibitory effect increased with the rise of the asciaticoside concentration in a dose-dependent manner. When the concentration of asciaticoside reached 25 mg/L, the expression of α-SMA was down-regulated with an activation index of 1. 673, showing a significant difference when compared with that in control group(P＜0.05). Conclusions Asciaticoside can effectively inhibit the DNA and collagen synthesis of capsulederived fibroblasts. The trans-differentiation of fibroblast to myo-fibroblasts is also prevented by it.     

经皮肾镜钬激光碎石病人的护理

经皮肾镜钬激光碎石在临床上是效果较好的一种碎石方法,集多种功能于一身,针对泌尿系碎石、泌尿系肿瘤息肉切除、泌尿系狭窄切开、前列腺切除的治疗均有良好的疗效[1].我院自2009年开展了这项新技术,现将30例经皮肾镜钬激光碎石病人的护理总结如下. 1 手术优点     

高血压病微观辨证研究进展

高血压病是当前最常见的心血管疾病,因其患病率高、并发症多、控制率低,一直是中西医学研究的重点.随着现代科技水平、科技手段的提高和病证结合研究的深入,高血压病微观辨证已成为近年来的研究热点.所谓微观辨证,即是在临床上收集辨证素材的过程中引进现代医学的先进技术,发挥它们在较深层次上、微观认识机体结构、代谢和功能的特点,从而更完整、更准确、更本质地阐明证的物质基础.