Genes Identification,Molecular Docking and Dynamics Simulation Analysis of Laccases from Amylostereum areolatum Provides Molecular Basis of Laccase Bound to Lignin

An obligate mutualistic relationship exists between the fungus Amylostereum areolatum and woodwasp Sirex noctilio. The fungus digests lignin in the host pine, providing essential nutrients for the growing woodwasp larvae. However, the functional properties of this symbiosis are poorly described. In this study, we identified, cloned, and characterized 14 laccase genes from A. areolatum. These genes encoded proteins of 508 to 529 amino acids and contained three typical copper-oxidase domains, necessary to confer laccase activity. Besides, we performed molecular docking and dynamics simulation of the laccase proteins in complex with lignin compounds (monomers, dimers, trimers, and tetramers). AaLac2, AaLac3, AaLac6, AaLac8, and AaLac10 were found that had low binding energies with all lignin model compounds tested and three of them could maintain stability when binding to these compounds. Among these complexes, amino acid residues ALA, GLN, LEU, PHE, PRO, and SER were commonly present. Our study reveals the molecular basis of A. areolatum laccases interacting with lignin, which is essential for understanding how the fungus provides nutrients to S. noctilio. These findings might also provide guidance for the control of S. noctilio by informing the design of enzyme mutants that could reduce the efficiency of lignin degradation.     

Effect of coupling agent content on properties of composites made from polylactic acid and chrysanthemum waste

Chrysanthemum flower is among one of the highly sought after and widely planted flower crops, in particular for cultural and religious ceremonies. However, the chrysanthemum stem and stalk have little value and usually discard as by‐product waste from floristry. The objective of this research is to investigate the potential value of utilizing chrysanthemum stem and stalk as reinforcing fillers for thermoplastic composites. In this study, 2‐mm thick composite sheet containing predefined formulations of polylactic acid (PLA), chrysanthemum waste filler (CWF) ranging from 15 to 60 phr, and maleated polyethylene (MAPE) coupling agent up to 5 phr were prepared with the aid of Haake internal mixer and compression molding. The effect of MAPE loading on tensile, thermal, and morphological properties of PLA/CWF composites was investigated. The findings revealed that PLA/CWF composite attained improved tensile modulus compared to the neat PLA, and the tensile modulus increases with higher concentration of CWF. However, both tensile strength and elongation at break reduces with increase loading of CWF. Overall, PLA/CWF composites with MAPE shows better performance compared to those without MAPE, where an optimum strength of 21.8 MPa can be achieved with 60 phr CW and 3 phr MAPE. The measured tensile strength is comparable to alternatives natural fiber thermoplastic composites demonstrating its potential to be used in non‐structurally demanding application. J. VINYL ADDIT. TECHNOL., 26:10–16, 2020. © 2019 Society of Plastics Engineers     

Effects of ethylene-octene copolymer (POE) on the brittle to ductile transition of high-density polyethylene/POE blends

Three kinds of ethylene-octene copolymers (POE) were melt-blended with high-density polyethylene (PE-HD) in different proportions. Detailed characterizations were conducted to analyze their structural differences of POE and its effects in toughening PE-HD. The higher molecular weight POE can improve the toughness of PE-HD. 60:40 PE-HD/POE is elongated to break up to 700% while impact strength is 84.7 kJ/m² at −30°C, which is 21-fold of PE-HD. In the brittle to ductile transition (BDT) during impact, the fracture mechanism changes from the crazing mode to the shear yield-plastic deformation mode. The BDT temperature decreases as the POE molecular weight and its content increase. The interface strength in tension is estimated to access their effects. The Boltzmann-type models were successfully extended to describe the typical S-shaped curves in BDT of notched impact strength vs POE content or temperature. The supplementary decay model is suggested for the attenuation in toughening. Transition map in impact is proposed to select the use range of composition (c ) and temperature (T ) for high toughness. The curves are converted into 3D graph of T -c -impact strength for illustrating their coupling-separate effects, and further into the contour map of impact strength in T -c space for finding their partial equivalence.     

Road Extraction from High Resolution Remote Sensing Image based on Shape Analysis with Gestalt Theory

Simulating the psychological experience of human vision，a road extraction model based on the format tower is proposed to extract the road in the high resolution remote sensing image from the perspective of morphology.Firstly，based on the spectral and texture information，the suspected road targets are extracted by using segmentation technology.Then these targets are classified according to their reliability and extract the road targets for each category.Finally，three types of identified road information are verified and merged，and the continuous smooth road extraction results are obtained.Experiments on real high resolution images show that the results are consistent with the visual perception of the human eye，and the overall classification accuracy is higher，indicating that the algorithm is effective and feasible and has good use value.     

2A12铝合金化学铣切加工工艺探讨

为了精确加工2Al2铝合金,探讨了化铣时间和温度对其化铣速率及表面粗糙度的影响,采用现代技术对化铣工件的力学性能进行了测试与分析.结果表明:延长非化铣部位保护胶固化时间并使保护部位向非加工面位移3～5 mm可很好地解决未化铣区域的腐蚀问题;随化铣时间的延长,化铣速率降低,且化铣温度与化铣速率呈现正相关性;化铣表面的粗糙度随化铣时间的延长而增加,随化铣温度的升高呈先减小后增加的趋势;化铣后材料的抗拉强度和屈服强度均有所提高.     

Dandelion-Like Bi2S3/rGO hierarchical microspheres as high-performance anodes for potassium-ion and half/full sodium-ion batteries

Sodium-ion batteries (SIBs) and potassium-ion batteries (PIBs) have been considered as attractive alternatives for next-generation battery systems, which have promising application potential due to their earth abundance of potassium and sodium, high capacity and suitable working potential, however, the design and application of bi-functional high-performance anode still remain a great challenge up to date. Bismuth sulfide is suitable as anode owing to its unique laminar structure with relatively large interlayer distance to accommodate larger radius ions, high theoretical capacity and high volumetric capacity etc. In this study, dandelion-like Bi₂S₃/rGO hierarchical microspheres as anode material for PIBs displayed reversible capacity, and 206.91 mAh·g⁻¹ could be remained after 1,200 cycles at a current density of 100 mA·g⁻¹. When applied as anode materials for SIBs, 300 mAh·g⁻¹ could be retained after 300 cycles at 2 A·g⁻¹ and its initial Coulombic efficiency is as high as 97.43%. Even at high current density of 10 A·g⁻¹, 120.3 mAh·g⁻¹ could be preserved after 3,400 cycles. The Na₃V₂(PO₄)₃@rGO//Bi₂S₃/rGO sodium ion full cells were successfully assembled which displays stable performance after 60 cycles at 100 mA·g⁻¹. The above results demonstrate that Bi₂S₃/rGO has application potential as high performance bi-functional anode for PIBs and SIBs.

     

国外某高磷鲕状赤铁矿直接还原—磁选降磷研究

以国外某高磷鲕状赤铁矿为研究对象进行脱磷研究，该高磷矿铁品位为55.81%，磷含量为0.72%，铁矿物主要为磁铁矿和赤铁矿，48.61%的磷存在于磷酸盐中，47.22%的磷分布于铁矿物中。研究了脱磷剂用量、秸秆炭用量、还原温度以及还原时间对粉末还原铁指标的影响。结果表明：无脱磷剂碳酸钙时，无法获得合格的指标；在碳酸钙用量为25%，秸秆炭用量为12.5%，还原温度1 200 ℃，还原时间为75 min，还原产品两段磨矿两段磁选的条件下，可获得铁品位、铁回收率以及磷含量分别为94.27%、87.34%以及0.077%的粉末还原铁，该产品可作为电炉炼钢的优质原料。不加添加剂时，部分含磷矿物被还原成单质磷进入到金属铁中，故粉末还原铁磷含量较高，当碳酸钙用量为25%时，含磷矿物的还原受到抑制而保留在脉石相中，可实现降磷目的，而还原时间过长时，磷仍在脉石相中，铁颗粒将部分含磷矿物包裹，磨矿难以分离，导致磷含量升高。