汞置换法测定碳氧血红蛋白含量的研究

本文对测定血中碳氧血红蛋白的三种方法进行了对比试验,试验结果表明汞置换法应用范围较广。     

缺血再灌损伤对谷胱甘肽过氧化酶活性的影响

<正> 结扎Langendorff法制备的大鼠心脏冠状动脉左前降枝15min,随后除去结扎再灌30min。在这个模型上谷胱甘肽过氧化酶(GPO)的活力(x±SD) 为291±40U(n=20),正常灌注心脏为232±57U(n=20)(1U=1μmolGSH·min~(-1)·g~(-1)组织湿重,37℃)。两组比较具有显著性差别(p<0.001)。但是Guarnieri等用     

儿童尿路结石导致肾功能损害6例报告

儿童尿路结石导致肾功能损害６例报告江苏连云港市第二医院赵连华，汪长胜尿路结石是泌尿系统常见病，但发生于儿童的尿路结石并不多见，一但发生容易引起尿路梗阻和继发感染，根据梗阻的部位及时间的长短可引起不同程度的肾功能损害，早期诊断及时治疗，在儿童期就显得十...     

靶肌肉注射碱性成纤维细胞生长因子对坐骨神经损伤再生及功能恢复的影响

[目的]探讨靶肌肉注射碱性成纤维细胞生长因子(bFGF)对大鼠坐骨神经损伤后再生及功能恢复的影响.[方法]60只SD大鼠按随机数表分为正常组20只和模型组(用硅胶管桥接坐骨神经6 mm缺损)40只,后者又随机分为bFGF组和对照组,分别于左胫前肌及腓肠肌各注射0.1ml的100 U bFGF和等量生理盐水,每日1次,连续30 d.术后10周,进行足迹分析、电生理、霍乱毒素-辣根过氧化物酶逆行神经标记(CB-HRP)和存活前角神经元计数、肌肉和神经组织学检查及图像分析等.[结果]bFGF组的坐骨神经功能指数恢复率,复合肌肉动作电位的潜伏期、波幅和神经传导速度,再生神经干中的有髓神经纤维密度、髓鞘厚度、轴突直径及许旺细胞数,被CB-HRP标记和存活的脊髓前角运动神经元数明显优于对照组,差异均有显著性(P＜0.05或0.01).[结论]靶肌肉注射bFGF能明显促进损伤后坐骨神经结构的再生、成熟,提高神经功能恢复,是临床应用bFGF的另一给药途径.     

原发型肝细胞癌动脉化疗栓塞术和全身静脉化疗的疗效比较及毒副反应观察

目的比较原发型肝细胞癌动脉化疗栓塞术和全身静脉化疗的疗效及毒副反应。方法58例中晚期原发型肝细胞癌患者根据接受治疗情况分为两组:动脉化疗栓塞组(动脉组)30例和全身静脉化疗组(静脉组)28例,两组所用的化疗药物相同。结果动脉组肿瘤缩小率、血清AFP下降率、生存率均优于静脉组(P<0.001,P<0.01)。不良反应发生率动脉组肝痛及发热均明显高于静脉组(P均<0.001),动脉组恶心呕吐、白细胞下降、ALT升高与静脉组比较均无显著性差异(P均>0.05)。结论动脉化疗栓塞术是中晚期肝癌安全、有效的治疗方法。     

抑郁发作自杀未遂患者脑源性神经营养因子水平及其相关因素研究

Objective To investigate the possible role of brain-derived neurotrophic factor(BDNF)in the pathophysiology of attempted suicide in depressive episode patients.Methods The subjects were 23 depressive episode patients who had recently attempted suicide.24 depressive episode patients without suicide attempt.and 30 sex-age-and education matched healthy controls.The Hamihon Depression Rating Scale-24 items(HAMD24),Beck Helpless Rating Scale(BHS)and Self-rating Idea of Suicide Scale (SIOSS)was used to evaluate the severitv of symptoms.The seruln level of BDNF was detected with sandwich ELISA.Pearson correlation coefficients wag used to measure the correlations between serum BDNF and the related illness variables.Results There were significant difierences in the serum level of BDNF between patients with attempted suicide,pailents without suicide attempt and normal controls(F=21.927,P＜0.05).Post hoc analysis showed the serum BDNF levels in patients with attempted suicide [(57 ±16)ng/L]were lower than that in patients without[(75±28)ns/L;P＜0.05],and were lower in patients without suicide attempt[(75 ±28)ng/L]than that in normal controls[(111 ±39)ng/L;P＜0.01].In patients with attempted suicide,the serum BDNF was negatively correlated to the duration of depressive episode(r=-0.541,P=0.008),the mean BHS score(r=-0.494,P=0.017)and the mean SIOSSscore(r=-0.754.P=0.000).Conclusion The results suggest that the decreased serum brain-derived neurotrophic factor is related to the pathophysiology of attempted suicide in depressive episode patients.     

创伤性肉毒中毒(颅脑损伤并发肉毒中毒的首例报告)

报告1例由于头皮撕裂伤感染肉毒杆菌引起的创伤性肉毒中毒,伤后11天出现全身无力、视物模糊、上睑下垂、咽下困难、呼吸肌麻痹等肉毒中毒的临床症状。伤口分泌物检出A型肉毒毒素和肉毒杆菌,血清中也检出A型肉毒毒素。经用肉毒抗毒素、人工呼吸机和创口局部积极治疗,挽救了病人的生命。结合复习文献,讨论本病的诊断要点、治疗原则和预防措施,并指出本例可能系颅脑损伤并发肉毒中毒的首例报告。     

腹腔镜胆囊切除术致胆管损伤的防治(附12例报告)

目的 探讨腹腔镜胆囊切除术致胆管损伤的原因、预防和处理原则.方法 回顾分析12例腹腔镜胆囊切除术致胆管损伤的临床资料.结果 10例行Roux-en-Y胆肠内引流术愈合,2例行Roux-en-Y胆肠内引流术后发现胆总管横断,改行胆总管空肠Roux-en-Y吻合术.但未放置胆肠支撑管,又发现吻合口狭窄,再次行胆肠吻舍并放置胆肠支撑管,全部愈合.结论 预防损伤十分重要,胆管损伤的及时发现和正确处理是治疗成功的关键.