胰岛素泵治疗糖尿病的护理体会

美国糖尿病控制与并发症试验研究结果显示 :强化治疗能有效地延缓 1型糖尿病患者糖尿病性视网膜病变、肾脏病变和神经病变的发生 ,并减慢其发展[1] ,英国前瞻性糖尿病研究证实 ,这一结论也适用于 2型糖尿病患者[2 ] 。胰岛素泵在糖尿病强化治疗中具有重要作用。胰岛素泵治疗 (持续皮下胰岛素注射 )是一种通过与机体的连接装置持续释放胰岛素的仪器 ,它通过设定一个持续的基础输注量及餐前大剂量 ,使胰岛素在体内的浓度更符合生理水平 ,胰岛素吸收更稳定、更有预测性 ,有效地防止或延缓各种并发症的发生。我科自 2 0 0 0年 9月引进美国Ｍｉｎ…     

观察健康教育和心理护理干预对伴更年期综合征女性患者的临床影响

目的:探讨健康教育和心理护理干预应用于伴有更年期综合征女性患者中的临床疗效。方法:选取本院2016年8月至2017年8月收治的75例伴有更年期综合征女性患者临床资料,参照组(n=35)采取常规护理,研究组(n=40)予健康教育和心理护理干预,比较两组HAMA、HAMD评分及治疗效果。结果:研究组HAMA、HAMD评分分别为(13.6±4.7)分、(10.2±4.3)分显著优于参照组,比较差异均具有统计学意义(P<0.05);而研究组的总治疗有效率为92.5%(3/40),显著优于参照组,比较差异均具有统计学意义(P<0.05)。结论:伴有更年期综合征的女性患者应用健康教育和心理护理干预,能有效改善患者临床症状,疗效良好。     

加味知柏地黄汤治疗肾阴虚型绝经前后诸症60例疗效观察

目的:观察加味知柏地黄汤治疗肾阴虚型绝经前后诸症的疗效。方法:将120例绝经前后诸症(肾阴虚型)患者随机分为两组,治疗组60例以加味知柏地黄汤进行治疗,对照组60例以克龄蒙进行治疗。结果:治疗组有效率为95.0%。结论:本方具有滋阴降火之功,对治疗绝经前后诸症(肾阴虚型)疗效确切,疗效相当于克龄蒙,且40~49岁年龄组疗效优于50~54岁年龄组。     

Glutamate enhances the surface distribution and release of Munc18 in cerebral cortical neurons

Objective

Munc18 is considered as an intracellular protein that plays an important role in exocytosis of neurotransmitters. Previous studies have demonstrated the presence of autoantibodies against Munc18 in a subgroup of Rasmussen’s encephalitis patients. However, the machinery of Munc18 autoimmunity is still elusive. The present study was aimed to investigate Munc18 release from primary cultured neurons, Munc18 distribution on the outer plasma membrane of neurons, and the neurotoxicity of Munc18 antibody.

Methods

The cerebral cortical neurons from embryonic day 17 Sprague-Dawley rats were prepared and cultured in neurobasal medium. The proteins in culture medium were precipitated with 10% trichloroacetic acid, and analyzed by immunoblotting. The proteins on neuronal surface were biotinylated with EZ-Link-sulfo-NHS-LC-Biotin, and collected with avidin-conjugated agarose beads followed by immunoblotting analysis. For cell surface immunofluorescent staining, the living neurons were labeled with anti-Munc18 antibody at 4 °C. Neuronal injury was assessed by lactate dehydrogenase(LDH) release.

Results

Munc18 was detected in culture medium by immunoblotting analysis. After treatment with 50 μmol/L glutamate for 1 h, Munc18 content in medium was increased. Meanwhile, β-actin and syntaxin1 were not detected in culture medium, and LDH release was not significantly increased. Moreover, glutamate enhanced Munc18 distribution on outer plasma membrane. Living neuron staining also demonstrated the localization of Munc18 on neuronal surface after glutamate treatment, especially at contacting regions between neurons. Glutamate-induced increase of surface Munc18 distribution was suppressed by NMDA receptor antagonist MK801, but not by AMPA receptor antagonist NBQX. Moreover, compared with c-Fos antibody, Munc18 antibody could induce neuronal injury, when culture medium contained the components of serum.

Conclusion

A portion of Munc18 can be released from neurons or distributed on neuronal surface, which can be enhanced by glutamate treatment via activation of NMDA receptors. Besides, Munc18 antibody-induced neuronal injury depends on the serum components.     

多聚谷氨酸化甲氨蝶呤浓度与类风湿关节炎疗效相关性研究

目的 探讨经甲氨蝶呤(methotrexate,MTX)治疗的类风湿关节炎(rheumatoid arthritis,RA)患者红细胞内多聚谷氨酸化甲氨蝶呤(methotrexate polyglutamate,MTXPG)的浓度与其疾病活动度的关系.方法 选取76例经MTX治疗3个月的RA患者进行横断面研究,采用EL...     

云克联合甲氨蝶呤治疗类风湿关节炎53例临床分析

目的探讨云克(99锝-亚甲基二磷酸盐)、甲氨蝶呤联合治疗类风湿关节炎(rheumatoid arthritis,RA)的疗效及其安全性。方法选取了53例活动期RA的临床资料进行回顾性分析,其中云克+甲氨蝶呤组22例,云克+甲氨蝶呤+来氟米特组31例,两组均接受相应药物治疗l2周。观察4、12周结束时临床症状改善、实验室指标变化及不良反应。结果 4、12周时两组的各项临床指标及实验室指标较治疗前均有显著性差异(P<0.05),但两组之间无显著差异(P>0.05)。不良事件两组均较少,两组之间总体比较差异无显著性意义(P>0.05)。结论云克(99锝-亚甲基二磷酸盐)和甲氨蝶呤联合治疗类风湿关节炎起效快,副作用少较小。     

云克治疗类风湿关节炎的护理

类风湿性关节炎（RA）是一种病因尚未明了的慢性全身性炎症性疾病,以慢性、对称性、多滑膜关节炎和关节外病变为主要临床表现,属于自身免疫炎性疾病。目前尚无根治的有效方法。云克（锝亚甲基二膦酸盐）用于自身免疫性疾病及骨科疾病,具有抗炎镇痛免疫调节及破骨修复作用。我院2008年8月-2009年2月对32例类风湿性关节炎患者采用云克治疗,效果满意。现将护理体会报告如下。     

正清风痛宁注射液离子导入疗法改善类风湿关节炎疼痛程度疗效观察

目的 观察正清风痛宁注射液离子导入疗法改善类风湿关节炎疼痛程度的疗效.方法 类风湿关节炎风寒湿痹型患者72例随机分为治疗组与对照组各36例,对照组采用传统治疗方法,治疗组在传统治疗方法基础上辅以正清风痛宁注射液离子导入;治疗后第10日比较两组患者关节疼痛程度.结果 治疗组疼痛VAS评分改善程度优于对照组,其总有效率亦高于对照组.结论 正清风痛宁注射液离子导入疗法能显著改善类风湿关节炎患者的关节疼痛程度.