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黄孢原毛平革菌低温产酶条件优化及酶学性质研究 总被引:1,自引:0,他引:1
以酶活力为评价指标,对白腐真菌产酶条件进行优化,并对其部分酶作用特性进行了研究.结果表明:该菌10℃条件下产LiP、MnP和Lac的最佳碳源是葡萄糖;最佳氮源是牛肉膏;最佳培养时间为72h~108h.LiP、MnP和Lac的最适反应pH值范围均为4.4~4.8,最适底物浓度为0.8 mmol/L~1.2mmol/L;金属离子Cu2+、Ca2+ 对LiP、MnP和Lac有激活作用,Fe2+ 对3种酶活有一定的抑制作用;而Na+ 则完全抑制LiP的活性,Zn2+、Mg2+对3种酶活影响不大. 相似文献
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本钢炼钢厂薄板坯连铸机在开浇初期,特别是在低碳钢的生产时,结晶器内几乎每个浇次开浇时都会产生冷齿,由于薄板坯铸机拉速高,冷齿现象极易产生漏钢的事故,并产生7m左右的废坯,增加了生产成本。因此主要对能够引起冷齿的生产和设备各种相关的因素进行分析,并通过生产实践的方法找出最佳的解决办法。 相似文献
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In order to analyze the seismic response characteristics of pile-supported structure, a computational model considering pile-soil-structure
interaction effect was established by finite element method. Then, numerical implementation was made in time domain. At the
same time, a simplified approximation for seismic response analysis of pile-soil-structure system was briefly presented. Furthermore,
comparative study was performed for an engineering example. Through comparative analysis, it is shown that the results obtained
by the simplified method well agree with those achieved by the finite element method. These results show that spectrum characteristics
and intensity of input earthquakes are two important factors that can notablely influence the seismic response characteristics
of superstructure. When the input ground motion acceleration amplitude gradually increases from 1 to 4 m/s2, the acceleration of pier top will increase, but it will not be simply proportional to the increase of input acceleration
amplitude. 相似文献
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