间充质干细胞对异体B淋巴细胞增殖和凋亡的影响

目的 探讨骨髓间充质干细胞(MSC)在体外对异体外周血B淋巴细胞增殖和凋亡的影响.方法 从骨髓中分离、培养MSC.从外周血中分离单个核细胞,L-亮氨酸甲酯去除单核细胞,以2-氨乙基硫脲溴化物(AET)处理绵羊红细胞(SRBC)的花环形成法,去除T淋巴细胞获得纯化的B淋巴细胞.用羊抗人IgM单克隆抗体(Anti-IgM,终浓度10μg/ml)刺激与不同比例的MSC(B:MSC 50:1、10:1、1:1)及不同浓度的MSC培养上清(12.5%、25%、50%)共培养的B淋巴细胞,72 h后四甲基偶氮唑蓝(MTT)比色分析测B淋巴细胞的增殖.B淋巴细胞与MSC等比例,加或不加Anti-IgM,共培养24、48 h后,流式细胞术检测B淋巴细胞的凋亡.结果 10:1、1:1组B淋巴细胞A值分别为0.418±0.103、0.365±0.114,显著低于对照组的0.679±0.049(P＜0.01),1:1组显著低于50:1组(P＜0.05);50%MSC上清组B淋巴细胞A值(0.504±0.099),同对照组相比差异有统计学意义(P＜0.05),并且显著低于12.5%组(0.669±1.023,P＜0.05).MSC不诱导B淋巴细胞的凋亡;B淋巴细胞与Msc共培养24、48 h,加或不加Anti-IgM,各组细胞凋亡率为(1.90±0.75)%、(2.33±1.01)%、(2.33±0.75)%、(1.39±0.63)%,组间比较差异无统计学意义(P＞0.05).结论 MSC及其上清抑制B淋巴细胞增殖,作用机制与MSC细胞数量和MSC分泌的细胞因子有关,但是在体外MSC不诱导B淋巴细胞的凋亡.     

黄连素儿童不宜

春夏交接，家里的小孩难免会不小心患上腹泻。而由于腹泻实在太常见了，家长就会根据自己的用药经验，给孩子治疗。不少人认为中药不良反应少，便用黄连素来治疗腹泻，觉得这样既安全又有效。但实际上，人们在不知不觉中已经走进了认识的误区。     

加强医院国有资产管理的必要性

国有资产是我国社会主义制度赖以存在和发展的物质基础,是全国人民的共同财产,国家的命根子。因此,加强国有资产的管理,防止国有资产的流失是当前一项重要任务。     

云南一种骨碎补习用品的生药鉴定

中国药典中骨碎补项下收载的品种为水龙骨科植物槲蕨的干燥根茎。而在云南省则习用多种植物。本文对其中的川滇槲蕨进行了药材性状、显微特征和薄层色谱鉴定,为利用和鉴别川滇槲蕨提供了一定的依据。     

斜角肌止点封闭治疗臂丛神经卡压症疗效分析

臂丛神经卡压症是指由于臂丛神经受压迫、刺激,其相应支配区出现疼痛、运动及感觉障碍的一类疾病.目前临床上对以臂丛神经下干卡压为主的胸廓出口综合征已有较清楚的认识,但是,对于上干卡压为主的臂丛神经卡压症病例,常常被错误地诊断为“神经根性颈椎病“或“肩周炎“,由于对发病部位的判断不正确,治疗效果不好.从1999年5月开始,采用颈部前中斜角肌起始部及止点封闭的方法治疗臂丛神经卡压症,取得较好疗效.现将这部分病例资料汇报如下.……     

Repair of sheep metatarsus defects by using tissue-engineering technique

Tissue-engineering bone with porous β-tricalcium phosphate (β-TCP) ceramic and autologous bone marrow mesenchymal stem cells (MSC) was constructed and the effect of this composite on healing of segmental bone defects was investigated. 10-15 ml bone marrow aspirates were harvested from the iliac crest of sheep, and enriched for MSC by density gradient centrifugation over a Percoll cushion (1.073g/ml). After cultured and proliferated, tissue-engineering bones were constructed with these cells seeded onto porous β-TCP, and then the constructs were implanted in 8 sheep left metatarsus defect (25 mm in length) as experimental group. Porous β-TCP only were implanted to bridge same size and position defects in 8 sheep as control group, and 25 mm segmental bone defects of left metatarsus were left empty in 4 sheep as blank group. Sheep were sacrificed on the 6th, 12th, and 24th week postoperatively and the implants samples were examined by radiograph, histology, and biomechanical test. The 4 sheep in blank group were sacrificed on the 24th week postoperatively. The results showed that new bone tissues were observed either radiographic or histologically at the defects of experimental group as early as 6th week postoperatively, but not in control group, and osteoid tissue, woven bone and lamellar bone occurred earlier than in control group in which the bone defects were repaired in “creep substitution” way, because of the new bone formed in direct manner without progression through a cartilaginous intermediate. At the 24th week, radiographs and biomechanical test revealed an almost complete repair of the defect of experimental group, only partly in control group. The bone defects in blank group were non-healing at the 24th week. It was concluded that engineering bones constructed with porous β-TCP and autologous MSC were capable of repairing segmental bone defects in sheep metatarsus beyond “creep substitution” way and making it healed earlier. Porous β-TCP being constituted with autologous MSC may be a good option in healing critical segmental bone defects in clinical practice and provide insight for future clinical repair of segmental defect.     

心室分离7例心电图及临床病因分析

心室分离是严重器质性心脏病并发的严重室性心律失常，临床十分罕见。2001年6月-2007年2月我们在临床实践中收治3例，经文献复习见到4例，现就该7例心电图表现结合临床病因分析如下。     

洗牙会损伤牙齿吗?

不少人担心洗牙会损伤牙齿,真的如此吗?事实上,洗牙不但能清除牙面的一些污垢而不损伤牙齿,还有预防龋齿、去除口臭的好处。因此,在发达国家,洗牙是很普及的常规口腔保健,人们每年都要找自己的牙医去洗牙1～2次。     

2022《美国心脏协会（AHA）心脏外科随机对照试验设计、执行和分析方法学标准的科学声明》解读

心脏外科在开展随机对照试验（RCT）时面临特别的挑战。为此美国心脏协会（AHA）制定了相关方法学标准的科学声明，旨在系统性总结心脏外科RCT设计，实施的核心建议、解读关键概念和标准，并为其提供具体的方法学建议：包括评估研究问题和临床均势，筛选代表性患者入组的可行性，实践差异对试验干预效果的影响，组间交叉的影响以及随访的持续时间。同时建议预先确定试验干预措施和研究终点，并确保试验干预措施的充分实施；尽一切努力保证随访的完整性；根据特定的研究问题和试验环境采用合适的试验设计和分析策略。本文笔者根据实践体会，对此科学声明进行解读。     

忧郁症心理治疗的探讨

当今世界是一个竞争激烈、节奏快、高效率的社会.不可避免地给人带来许多压力.我们应该正确认识忧郁症,忧郁症是每个人都可能患的心理疾病,它不能说明心胸狭窄,也不能说明品质低劣或意志薄弱.